FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543058
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG44980.8282724865483981Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG34930.6432093809501012Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTGTAGTGTCCGGTGCGCTCCCGACGACCCTTGAAAATCCGCTGGAAGGAATCAGATTCGCGCCAGGTCGTA22440.4132155313060483No Hit
CTACAGAGGTGTAGTGCTCTGCCAGCCATTAAACCCTAGCTCCGGACAAACCGTTTTCAGGGTGAGAGACTGTTA21270.3916708712513212No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT18390.3386378618858391Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14020.2581676358694651Clontech Universal Primer Mix Long (96% over 26bp)
GCTTAGGAGTGTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACCCA13540.2493288009752181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.24398867155994386No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13190.242883817198163Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11330.2086333319829558Clontech Universal Primer Mix Long (96% over 26bp)
CATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTTAGGAGTGTGTAACAACTCACCGGCCGAATGC11310.2082650471956955No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTGAAAGGGAAG10820.19924206990781834No Hit
ACCCATACCTCGCCGTCAGGGTTGACATGATGCCCTGACGAGTAGGCAGGCGTGGGGGTTTGTGACGAAGCCTTG10740.19776893075877716No Hit
CTCCTAAGCGGATTCCGACTTCCATGGCCACCGTCCGGCTGTCTAGATGCATTGACACCTTTTGTGGTGTCTGAT10460.19261294373713306No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGATGAAATTTACCACCCATTTTGAGCTGCATT9880.18193268490658457No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9790.18027540336391323Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9230.16996342932062505Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT8740.16094045203274787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.15762588894740526No Hit
GGTTAAGGTCGCTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC8540.15725760416014495No Hit
ATCCTAAGCCCTAGGGAAGTTCCGTAAAAAGTGCGCACTACGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATT7950.1463932029359663No Hit
GTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGCTACCCATACCTCGCCG7920.14584077575507587No Hit
GTTCTAAGTTGGTTGTTAATCGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACACCAGGTGCTGGGTGCC7820.14399935181877443No Hit
GTCTAGATGCATTGACACCTTTTGTGGTGTCTGATGAGCGTGCATTCCGGCACCTTAACCTCGCGTTCGGTTCAT7750.1427103550633634No Hit
TCCTACCTCCGTTCACTTTCATTACGCGCAAGGGTTTGACACCCGAACACTCGCATAGATGTTAGACTCCTTGGT7670.14123721591432223No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT7230.1331349505945958No Hit
CATCAGGATCGGTCGATAGTGCAGGCTCAGGCCTTCCTACCTCCGTTCACTTTCATTACGCGCAAGGGTTTGACA7170.13203009623281492No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6960.12816310596658184Clontech Universal Primer Mix Long (96% over 26bp)
TGGGTGAGTCGATCCTAAGCCCTAGGGAAGTTCCGTAAAAAGTGCGCACTACGCGCCGTCGGGCGAAAGGGAAGC6830.12576925484938994No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGCGTATAGGGGCGAAAGACTAATCGAACCATCTGGTAGCTGGTTC6730.12392783091308847No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6690.12319126133856788Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGCACGTTG6640.12227054937041716No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6580.12116569500863629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATGGGTAGCACGCTTAAGCGCCATCCATTTTCAGGGCCAATGCATTCGGCCGGTGAGTTGTTACACACTCCT6530.12024498304048556No Hit
CCCCCAAGGCTTCGTCACAAACCCCCACGCCTGCCTACTCGTCAGGGCATCATGTCAACCCTGACGGCGAGGTAT6420.11821941671055394No Hit
CATCAGACACCACAAAAGGTGTCAATGCATCTAGACAGCCGGACGGTGGCCATGGAAGTCGGAATCCGCTTAGGA6400.11785113192329365No Hit
GAGGTAGGAAGGCCTGAGCCTGCACTATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTG6370.1172987047424032No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6320.11637799277425247Clontech Universal Primer Mix Long (96% over 26bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAATCTGATTCCTTCCAGCGG6290.11582556559336203No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6140.11306342968890983Clontech SMART CDS Primer II A (96% over 26bp)
GCGCTTAAGCGTGCTACCCATACCTCGCCGTCAGGGTTGACATGATGCCCTGACGAGTAGGCAGGCGTGGGGGTT6010.11066957857171794No Hit
CCCTAGGGAAGTTCCGTAAAAAGTGCGCACTACGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCACC5980.1101171513908275No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACCAGATGGTTCGATTAGTCTTTCGCCCCTATACGCAAA5930.10919643942267677No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT5880.10827572745452604No Hit
CTACGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGCGGTTTCTTCACGGCAACGTAAC5590.10293559803925179No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5550.1021990284647312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAGCGACCTTAACC5520.10164660128384076No Hit
GCACTACACCTCTGTAGTGTCCGGTGCGCTCCCGACGACCCTTGAAAATCCGCTGGAAGGAATCAGATTCGCGCC5480.1009100317093202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)