FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002153448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686459
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG56390.8214620246802796Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44220.6441753986763958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.4311983672732093No Hit
GTCTATACCACCCAACACCTTTTGTGGAGTCTGATGAGCATGCTGTTAGGCGCCTTATCTCGCCGTTCGGTTCAT25700.37438506888248246No Hit
GTGTGAGGTTGAGCAAGTAAGATGCGACCCGAAAGATGGTGAACTACGGCGGCATAGGATGAAGCCGGTGGAAAC22390.32616660281240395No Hit
GGTATAGACAGCAGGACGGTGGCCATGGAAGTTGGCATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATTG21720.3164063694991252No Hit
GTACACGGGTGAGCCGATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACTGAA19990.29120457303349506No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT19730.2874170198074466Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAAGACGCATCCGATGTTGCCACTCCCCGAAAGGAACCCCGCTACTACGCTCAGGTGTCGGATCACAGCATC19360.2820270402165315No Hit
ACCTCACACTATGTGCGGGCGCGGTCGCCCCGCCCCGAGGAACGGTTCACCGTCTACGCTTTCGCTACGCCTCCG18880.27503463426074976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.2576992944953741No Hit
CTCTTCGACCACTCGACCACATCTCCGGACGAACCGGTTCCGTGGCCTCGAGCCGTTCAGACAACAAGATAACTT15620.2275445438110652No Hit
CCCGTGTACCATCGCGGTAAGCACGGAACCCTTCCCCACGTCAGTCTACCGGGATCACACCAGTATATTTGCTAC15600.22725319356290763No Hit
CTTCAGCGCCATCCATTTTCAGGGCTAGGCAATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATGCCAACT15490.22565076719804097No Hit
GTGCGATGGTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAACCGGTTCGTCCGGAGATGT15450.2250680667017258No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15230.22186321397199252Clontech Universal Primer Mix Long (96% over 26bp)
GCGCTGAAGCATGCGGCCGATTCTCGTCCCCGGCGGCTTGATACCCCGCCGGGAGTAGGAGGGCGTGAGGGTGTT15110.22011511248304708No Hit
GGGTACGACGATGCGAGAGGAGCAAGTGATCACCATGGTTTTCAAGGGATGCAACAGGTTACGGGACGGGGGACC14920.2173472851255501No Hit
CCATCGCACCGTCCCATGGGTCATATGCGTTGCCGCCAAAACTCCCTCAGTTAACATCGCAGCCGAATGACAACG14740.2147251328921319No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA14300.20831542743266532Clontech Universal Primer Mix Long (96% over 26bp)
GATTAGGAGCCATCGCGATGCTATGTTTTAATTAGACAGTCAGATTCCCCAAGTCCGCGCCAGTTCTGAGCCGAC14300.20831542743266532No Hit
TTGTTGCGCCGCCCACTGAAAGGGAAAGCCGTTGTCATTCGGCTGCGATGTTAACTGAGGGAGTTTTGGCGGCAA13920.20277977271767142No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13730.20001194536017447Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAAATGCGTGCGAACCAACGTGTGTCTGACAAGCACCGTTTGGTCAATGCTTTTGGCACAAGTGGGCCAATT12530.18253093047072003No Hit
GTCCTATGCAGTTGCATGCGGTAAAGCGAACGATTAGGCGTTTTGGGGACCTCAGTGTCCTCGGCGCATTCTCGA11770.17145962104073223No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11650.1697115195517868Clontech Universal Primer Mix Long (96% over 26bp)
GGCCACACCGCTCTTCGACCACTCGACCACATCTCCGGACGAACCGGTTCCGTGGCCTCGAGCCGTTCAGACAAC11510.16767206781468375No Hit
ATGGTACACGGGTGAGCCGATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACT11500.16752639269060496No Hit
CTCCTTAGCGGATGCCAACTTCCATGGCCACCGTCCTGCTGTCTATACCACCCAACACCTTTTGTGGAGTCTGAT11340.16519559070534437No Hit
CTCCTACTCCCGGCGGGGTATCAAGCCGCCGGGGACGAGAATCGGCCGCATGCTTCAGCGCCATCCATTTTCAGG11070.16126236235521713No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATTGCCTAGCCCTGAAAATGGATGGCGCTGAAGCATGCGGCCGA11020.1605339867348232No Hit
GTTATGGGTACGACGATGCGAGAGGAGCAAGTGATCACCATGGTTTTCAAGGGATGCAACAGGTTACGGGACGGG10950.15951426086627168No Hit
GAGTAGGAGGGCGTGAGGGTGTTGCGTCGAAGGGTGTCCGTGAGGGCATCTGGAGCCGCCCTTAGTGCCGATCTT10740.1564550832606172No Hit
GTCGAAGAGCGGTGTGGCCTGGTCCCCCGTCCCGTAACCTGTTGCATCCCTTGAAAACCATGGTGATCACTTGCT10530.15339590565496264No Hit
ATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACTGAAAGGGAAAGCCGTTGTC10500.15295888028272628No Hit
CCCATGGGACGGTGCGATGGTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAACCGGTTCG10070.14669484994733845No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10000.14567512407878694Clontech Universal Primer Mix Long (96% over 26bp)
AGGCCACACCGCTCTTCGACCACTCGACCACATCTCCGGACGAACCGGTTCCGTGGCCTCGAGCCGTTCAGACAA9660.1407221698601082No Hit
GCATAGGACACAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCCATTAGACGGTTCGATAGGTCTTTCGCCCC9560.13926541861932032No Hit
CCCCAAGTCCGCGCCAGTTCTGAGCCGACAGTTCAAGACGCATCCGATGTTGCCACTCCCCGAAAGGAACCCCGC9370.13649759126182337No Hit
GTCGTACCCATAACCGCATCAGGTCTCCGAGGTGAACAGCCTCTAGTCAAATGGAATAATGTGGATAAGGGAAGT9210.13416678927656275No Hit
CATTTAGAGTTCGAGAATGCGCCGAGGACACTGAGGTCCCCAAAACGCCTAATCGTTCGCTTTACCGCATGCAAC9070.13212733753945977No Hit
GTGTGGCCTGGTCCCCCGTCCCGTAACCTGTTGCATCCCTTGAAAACCATGGTGATCACTTGCTCCTCTCGCATC8930.13008788580235672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.1284854594374901No Hit
TCCCATGGGTCATATGCGTTGCCGCCAAAACTCCCTCAGTTAACATCGCAGCCGAATGACAACGGCTTTCCCTTT8690.12659168282446584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.12630033257630827No Hit
GTGTACCATCGCGGTAAGCACGGAACCCTTCCCCACGTCAGTCTACCGGGATCACACCAGTATATTTGCTACTTC8620.12557195695591433No Hit
ACTAGAGGCTGTTCACCTCGGAGACCTGATGCGGTTATGGGTACGACGATGCGAGAGGAGCAAGTGATCACCATG8420.1226584544743386No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8410.12251277935025981Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCAGACAACAAGATAACTTGTCCCCGCACCATCGCACCGTCCCATGGGTCATATGCGTTGCCGCCAAAACTCC8290.12076467786131437No Hit
GTCCCATGGGTCATATGCGTTGCCGCCAAAACTCCCTCAGTTAACATCGCAGCCGAATGACAACGGCTTTCCCTT8260.12032765248907801No Hit
GGATAGCTGTGTCCTATGCAGTTGCATGCGGTAAAGCGAACGATTAGGCGTTTTGGGGACCTCAGTGTCCTCGGC8200.11945360174460529No Hit
CATATGACCCATGGGACGGTGCGATGGTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAAC8130.11843387587605378No Hit
GGTCATATGCGTTGCCGCCAAAACTCCCTCAGTTAACATCGCAGCCGAATGACAACGGCTTTCCCTTTCAGTGGG8020.11683144951118711No Hit
GTTCTGACGTGCAAATCGATCGTGCGATGTCGCTGTAGGGGCGAAAGACCTATCGAACCGTCTAATGGCTGGTTC7980.11624874901487198No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7870.11464632265000532Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATCGCGTCAACGCTGATTAGGAGCCATCGCGATGCTATGTTTT7740.1127525460369811No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATTGCCTAGCCCTGAAAATGGATGGCGCTGAAGCATGCGGCC7690.11202417041658716No Hit
CCTTAGCGGATGCCAACTTCCATGGCCACCGTCCTGCTGTCTATACCACCCAACACCTTTTGTGGAGTCTGATGA7500.10925634305909021No Hit
GCGCAACAATGTGCGCTCATTACCGGGGGCTTCCCCACCCTTTAGGATCGGCTCACCCGTGTACCATCGCGGTAA7460.10867364256277505No Hit
TCCCCGAGAGGACCGCAATCTTCCACCTTTGCATTTCGCAAAGGCCGAACACGCCCTGCCCCCAGAGCCACCCCT7440.10838229231461749No Hit
GAGCTAGCTTGCCGACTTCCCTTATCCACATTATTCCATTTGACTAGAGGCTGTTCACCTCGGAGACCTGATGCG7350.1070712161979084No Hit
GTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAACCGGTTCGTCCGGAGATGTGGTCGAGT7240.10546878983304173No Hit
GGACACAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCCATTAGACGGTTCGATAGGTCTTTCGCCCCTACAG7130.1038663634681751No Hit
CTTTAGGATCGGCTCACCCGTGTACCATCGCGGTAAGCACGGAACCCTTCCCCACGTCAGTCTACCGGGATCACA7020.10226393710330843No Hit
CCGCTACTACGCTCAGGTGTCGGATCACAGCATCCCCGAGAGGACCGCAATCTTCCACCTTTGCATTTCGCAAAG6950.10124421123475694No Hit
TGTCTGAACGGCTCGAGGCCACGGAACCGGTTCGTCCGGAGATGTGGTCGAGTGGTCGAAGAGCGGTGTGGCCTG6930.10095286098659934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)