FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002153449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686459
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG38660.5631800296885903Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG29670.4322180931417608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATAGACAGCAGGACGGTGGCCATGGAAGTTGGCATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATTG16930.2466279850653863No Hit
GTCTATACCACCCAACACCTTTTGTGGAGTCTGATGAGCATGCTGTTAGGCGCCTTATCTCGCCGTTCGGTTCAT16590.24167503084670752No Hit
GTGTGAGGTTGAGCAAGTAAGATGCGACCCGAAAGATGGTGAACTACGGCGGCATAGGATGAAGCCGGTGGAAAC16340.23803315274473785No Hit
GTACACGGGTGAGCCGATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACTGAA14990.21836701099410163No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13910.20263409759359263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCACACTATGTGCGGGCGCGGTCGCCCCGCCCCGAGGAACGGTTCACCGTCTACGCTTTCGCTACGCCTCCG11630.1694201693036292No Hit
CTTCAGCGCCATCCATTTTCAGGGCTAGGCAATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATGCCAACT11620.16927449417955043No Hit
GTGCGATGGTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAACCGGTTCGTCCGGAGATGT11580.16869179368323525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.15893156036995654No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10560.15383293102719903Clontech Universal Primer Mix Long (96% over 26bp)
GCGCTGAAGCATGCGGCCGATTCTCGTCCCCGGCGGCTTGATACCCCGCCGGGAGTAGGAGGGCGTGAGGGTGTT10520.15325023053088385No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10200.14858862656036267Clontech Universal Primer Mix Long (96% over 26bp)
TTGTTGCGCCGCCCACTGAAAGGGAAAGCCGTTGTCATTCGGCTGCGATGTTAACTGAGGGAGTTTTGGCGGCAA10190.1484429514362839No Hit
ATGGTACACGGGTGAGCCGATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACT9480.13810001762669002No Hit
GGGTACGACGATGCGAGAGGAGCAAGTGATCACCATGGTTTTCAAGGGATGCAACAGGTTACGGGACGGGGGACC9430.1373716420062961No Hit
GTTCAAGACGCATCCGATGTTGCCACTCCCCGAAAGGAACCCCGCTACTACGCTCAGGTGTCGGATCACAGCATC9380.13664326638590216No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9360.13635191613774458Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTCGACCACTCGACCACATCTCCGGACGAACCGGTTCCGTGGCCTCGAGCCGTTCAGACAACAAGATAACTT9290.13533219026919305No Hit
CCATCGCACCGTCCCATGGGTCATATGCGTTGCCGCCAAAACTCCCTCAGTTAACATCGCAGCCGAATGACAACG8790.1280484340652537No Hit
GTCGAAGAGCGGTGTGGCCTGGTCCCCCGTCCCGTAACCTGTTGCATCCCTTGAAAACCATGGTGATCACTTGCT8420.1226584544743386No Hit
GTCGTACCCATAACCGCATCAGGTCTCCGAGGTGAACAGCCTCTAGTCAAATGGAATAATGTGGATAAGGGAAGT8380.12207575397802345No Hit
CTCCTTAGCGGATGCCAACTTCCATGGCCACCGTCCTGCTGTCTATACCACCCAACACCTTTTGTGGAGTCTGAT8310.12105602810947194No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATTGCCTAGCCCTGAAAATGGATGGCGCTGAAGCATGCGGCCGA8250.12018197736499922No Hit
GTCCTATGCAGTTGCATGCGGTAAAGCGAACGATTAGGCGTTTTGGGGACCTCAGTGTCCTCGGCGCATTCTCGA8090.11785117537973862No Hit
GGCCACACCGCTCTTCGACCACTCGACCACATCTCCGGACGAACCGGTTCCGTGGCCTCGAGCCGTTCAGACAAC7960.11595739876671439No Hit
CTCTAAATGCGTGCGAACCAACGTGTGTCTGACAAGCACCGTTTGGTCAATGCTTTTGGCACAAGTGGGCCAATT7880.11479199777408411No Hit
GTTATGGGTACGACGATGCGAGAGGAGCAAGTGATCACCATGGTTTTCAAGGGATGCAACAGGTTACGGGACGGG7810.1137722719055326No Hit
GAGTAGGAGGGCGTGAGGGTGTTGCGTCGAAGGGTGTCCGTGAGGGCATCTGGAGCCGCCCTTAGTGCCGATCTT7790.11348092165737501No Hit
GTGTGGCCTGGTCCCCCGTCCCGTAACCTGTTGCATCCCTTGAAAACCATGGTGATCACTTGCTCCTCTCGCATC7520.10954769330724777No Hit
CCCATGGGACGGTGCGATGGTGCGGGGACAAGTTATCTTGTTGTCTGAACGGCTCGAGGCCACGGAACCGGTTCG7470.10881931768685384No Hit
ATCCTAAAGGGTGGGGAAGCCCCCGGTAATGAGCGCACATTGTTGCGCCGCCCACTGAAAGGGAAAGCCGTTGTC7470.10881931768685384No Hit
GATTAGGAGCCATCGCGATGCTATGTTTTAATTAGACAGTCAGATTCCCCAAGTCCGCGCCAGTTCTGAGCCGAC7390.10765391669422354No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7220.10517743958488417Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTACTCCCGGCGGGGTATCAAGCCGCCGGGGACGAGAATCGGCCGCATGCTTCAGCGCCATCCATTTTCAGG7160.10430338884041145No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7090.10328366297185994Clontech Universal Primer Mix Long (96% over 26bp)
GCATAGGACACAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCCATTAGACGGTTCGATAGGTCTTTCGCCCC7080.10313798784778115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)