FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367763
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89062.4216683026840653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55411.5066768543872004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43741.1893529256613635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.6925655925147446No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA23890.6496031411534059RNA PCR Primer, Index 40 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.3151486147328578No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.2863257043258838No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.24091602472244353No Hit
ATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6940.18870848889094335RNA PCR Primer, Index 40 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.18000723291902665No Hit
TTATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5310.14438646628399268TruSeq Adapter, Index 8 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT4170.11338824188403945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3950.10740612840334672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)