FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002153484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455837
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT62910.4321225521813225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45360.31157334234533124No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC38170.2621859452672243No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA34690.23828217032538668No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC34450.23663363412250138No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA33910.23292442766600932No Hit
GATGTGTATCTATCACATAATCCAGCAGGAACTTCTGTTCAAAACATGTT33190.22797881905735326No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT31600.2170572667132378No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT30910.21231772512994243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29990.20599833635221526No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT27410.18827657217119773No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC27220.18697148101058017No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG25060.17213465518461202No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC23980.16471624227162793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.16368590714482462No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT22540.15482502505431583No Hit
CCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAGTGGGCACAG21930.1506349955386489No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA21880.15029155049638113No Hit
CATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTG20720.1423236255157686No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC20560.1412246013805117No Hit
GTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGA19950.13703457186484477No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT18760.12886057985887156No Hit
ACATAGTGTAGCTGCTTCTGTCCAGTTTTCTTTACAATGAAGTCGATTGT18690.12837975679969665No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA17980.12350283719949418No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17250.11848853958238456No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16990.11670262536259211No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA16790.11532884519352099No Hit
GACTAATGTTCATAGTCACTTCAGGGCTTCCAGGATGTAATTTTCCAAAC16510.1134055529568214No Hit
TATCTGTACTGGGGACTACACATGGTTTGTTTGGAAAATTACATCCTGGA16330.11216915080465739No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG16230.11148226072012182No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT16180.11113881567785405No Hit
GTACAGATATCAAACTTGCCATTGTTAAAAGCAGCCACATTTTGGACCTG16120.1107266816271327No Hit
GTACTGGACTCCATTGATGGTGAAGACGATGTCGGGCAGGCTGCTGATGG16040.11017716955950425No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA15130.10392646979023062No Hit
AACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGT14870.10214055557043818No Hit
ATACTAACCAGCCAGAGAAACAGAATCCTAACTATTTCTGAGGAAACTGC14800.10165973251126327No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT14700.10097284242672772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)