FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002153729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555378
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.23173406220628112No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12580.22651239336091816No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG10150.18275840958770423No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8580.15448937480418742Clontech Universal Primer Mix Long (96% over 26bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6920.12459982210314417No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6600.1188379806186057No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6330.11397642686602638No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTA5760.10371314672169224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)