FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362337
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG35180.9709193375228033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG28880.7970480519516363Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16400.45261731482018125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGATGAAATTTACCACCCATTTTGAGCTGCATT15740.43440222776034465No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15300.42225883638712025Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12240.3378070691096962Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11470.31655613420655354Clontech Universal Primer Mix Long (96% over 26bp)
CTCCACGGCAACGTAACTGAACGCGGAGACGACGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACAACC10930.30165288115759636No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGAATGTGGACTCTCCACGGCAACGTAA10410.2873016004437858No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9700.2677065825460828Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8590.23707211794544858Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC8360.23072443609126309Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8260.22796457441553028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCGGAACCCCGATGCTGCTCGG7890.21775308621531886No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG7180.1981580683176159Clontech Universal Primer Mix Long (96% over 25bp)
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG7080.1953982066418831No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7040.19429426197159Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6720.185462704609245Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6340.1749752302414603No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6250.17249135473330077Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTGGAGAGTCCACATTCAGGTGCCGGAATATTAACCGGCTTCCCTTTCGAGGGACGGCGCACAAGTGCGCAC6090.16807557605212825No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGACGTGA5890.16255585270066264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.1586920463546367No Hit
GCGCACAAGTGCGCACTTTGAAACGGAGTTTCCCTATCTCTTAGGATCGACTAACTCATGTCCAACTGCTGTTCA5670.15648415701405047No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGACGTGAAAACTATTCCTTCCCGCGG5420.14958450282471844No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5410.14930851665714515Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5340.14737661348413217Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGTGCGCTCCCGACGTCCCTTGAAAATCCGCGGGAAGGAATAGTTTTCACGTCAGGTCGTACTCATAACCG5300.14627266881383905No Hit
ACCCATACCTCGCCGCCAGGGTAGAAACGATGCCCTGGCGAGTAGGCAGGCGTGGGGGTCCGTGACGAAGCCTTG5280.14572069647869249No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG5010.1382690699542139No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCGGAA4600.12695363708370935No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAAC4540.1252977200782697No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC4530.1250217339106964No Hit
GCTCCGGGGACGTCCCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA4500.12419377540797655No Hit
GTGCCGGGCTGCTCCGGGGACGTCCCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT4410.12170989989981702No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTCAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATA4180.11536221804563154No Hit
GAATGTGGACTCTCCACGGCAACGTAACTGAACGCGGAGACGACGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCT4180.11536221804563154No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAATTAGCCCTGAAAATGGATGGCGCTCAAGCGTGCTACCCA4040.11149841169960562No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGCCGGCGCCTCACCCGAAACACCCTCTACAAATTACAACTCGGAC4000.11039446702931249No Hit
GCCGAAGCCTGCCAAGGGCGTCGGAACCCCGATGCTGCTCGGCGCCGGCCTGTTGCCAAGCTAGTGCCGGGCTGC3920.10818657768872625No Hit
GTATTTAGCTTTAGATGAAATTTACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGTTT3810.10515072984542014No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGCACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCGGAACCCCGA3790.10459875751027357No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC3780.10432277134270031Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3770.10404678517512703No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT3680.1015629096669675Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)