FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310956
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17510.5631021752273634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.3328445181955003No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT8470.2723858037793128No Hit
GTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACGACGATCCCGAGTCAGC8300.2669187923693384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.22157475655719777No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6600.21224867826959443No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTACGTCG6120.19681241075907846No Hit
GTCAGGAACGCACCGGACTCGTCGCAAGTGACGAGCTCTACCCGCCATTGAACCCTAGCTCCGGATAATCCGATT5140.16529669792510837No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA5000.16079445323454122No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4980.16015127542160307No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA4900.1575785641698504No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAAAATTATATGTTCCGTTGG4830.15532744182456681No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG4750.15275473057281416No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCACGCGATTCTTCGCGTCACTATCGAAAGGGAATCCGGTTAAG4720.15178996385340693No Hit
GTTCCTGACAGTCCTTGAAAATCCAACGGAACATATAATTTTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC4690.15082519713399967No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCT4690.15082519713399967No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA4590.14760930806930883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.14696613025637068No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACG4400.14149911884639627No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACGACGATC4100.13185145165232381No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3830.12316855117765857No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC3830.12316855117765857No Hit
GAGTACGACCAGGCGTGAAAATTATATGTTCCGTTGGATTTTCAAGGACTGTCAGGAACGCACCGGACTCGTCGC3360.10805387257361171No Hit
GCCTACAACCCTGAAATCGGATTATCCGGAGCTAGGGTTCAATGGCGGGTAGAGCTCGTCACTTGCGACGAGTCC3240.1041948056959827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)