FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310956
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACGACGATCCCGAGTCAGC7130.22929289031245578No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT6990.22479064562188866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.21353503389547074No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG6220.2000282998237693No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA5240.1685125869897992No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTACGTCG5180.1665830535509847No Hit
TTCCAGCACCTGGTTGTGGACGCCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG5140.16529669792510837No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4900.1575785641698504No Hit
GTTCCTGACAGTCCTTGAAAATCCAACGGAACATATAATTTTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC4650.14953884150812333No Hit
GTCAGGAACGCACCGGACTCGTCGCAAGTGACGAGCTCTACCCGCCATTGAACCCTAGCTCCGGATAATCCGATT4560.1466445413499016No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA4270.1373184630622982No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACG4240.13635369634289096No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCT4160.13378098509113828No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA4110.1321730405587929No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC3840.12349014008412766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.12284696227118949No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTACGCCGGACGCCTTGCGGCAGCTGAAGCCTTTCACGACGATC3570.11480723960946243No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAAAATTATATGTTCCGTTGG3510.11287770617064793No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3230.10387321678951363No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGCACGCGATTCTTCGCGTCACTATCGAAAGGGAATCCGGTTAAG3200.10290845007010638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)