FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191896
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39102.03756201275691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27201.4174344436569808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.7019427189727769No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.5487347313127945No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA7050.3673865010213866TruSeq Adapter, Index 2 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.365302038604244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.2579522241213991No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.25638887730854215No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.19802392962854878No Hit
ATACACATCTCCGAGCCCACGAGACTCATATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2630.1370534039271272TruSeq Adapter, Index 2 (96% over 29bp)
GCCTGAAGATATGCTCGAGAGTTAGTCACGCGTCACGATTAGTCGACTCCCTTTAGTGAGGGTTAATTTCGAGCA2460.12819443865427108No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.10265977404427398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1970.10265977404427398No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)