FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140805
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30132.1398387841340862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21531.529065018997905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.7975569049394553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.7748304392599694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.5688718440396293No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.40197436170590534No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.321721529775221No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.27768900252121725No Hit
CCTATGTATTCAGTATGCAATGACCTATAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTT1760.12499556123717198No Hit
GACCTATAAAGGCCGGGTTTCCCCATTCGGAAATCTGCGGATCAAAGCTCGTTTGCTAGCTCCCCGCAGCTTATC1720.12215475302723625No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.11860374276481658No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.11292212634494514No Hit
ACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGCTTTGATCCGCAGATTTCCGAATGGGGAAACCC1430.10155889350520224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)