FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002153857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625522
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG66411.0616732904678012Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56480.9029258763081074Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31810.5085352713413757Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.44666694376856453No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23300.3724888972729976Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21690.346750394070872Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA21440.34275373208296434Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG17390.2780078078788596Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.2701743503825605No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC16850.269375017984979Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT16270.2601027621730331No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT15570.24891210860689153Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG15270.2441161142214023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13830.22109534117105392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12970.20734682393265144Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACAGGATTTCA12880.20590802561700466No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11790.18848257934972712Clontech Universal Primer Mix Long (96% over 26bp)
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCAC11640.18608458215698248No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11590.18528524975940094Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCACCTCTTCGCGCTTTGACACGCTACATGAGG11060.17681232634503663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.16530193981986246No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAAGTGATGG9530.1523527549790415No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG9410.1504343572248458No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA8990.14371996508516088No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT8920.14260089972854673No Hit
TTCCGGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG8590.13732530590450856No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8480.13556677462982916Clontech Universal Primer Mix Long (96% over 26bp)
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCACCT8190.13093064672385624No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTGCACCCCATCACTTAACCTT8050.12869251601062792No Hit
GCCTCATGTAGCGTGTCAAAGCGCGAAGAGGTGGCACAGAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCA7940.12693398473594852No Hit
ACATGAGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCTGGGTTCTCTCAAGCGCCT7930.12677411825643223No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC7910.1264543852973996Clontech Universal Primer Mix Long (96% over 26bp)
GTTTAACGCTACTCATGTCAGCATTCGCACTTCCGATACCTCCAGCAGCCCTCACGAGCTGCCTTCTGCGGCCTA7790.12453598754320391No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT7770.12421625458417129Clontech SMART CDS Primer II A (96% over 26bp)
CTCATGTAGCGTGTCAAAGCGCGAAGAGGTGGCACAGAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACT7710.12325705570707345No Hit
CTACATGAGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCTGGGTTCTCTCAAGCGC7680.12277745626852453No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAAGTGATGGGGTGCAAGCTCTTGATCGAAGCCCCGGTA7600.12149852443239406No Hit
GAGTAAGTGCATGTGGTGGATGCCTTGGCGATCACAGGCGAAGAAGGACGCGGCAGCCTGCGAAAAGCTGCGGGG7520.12021959259626361No Hit
CTGTAATGGTTGCGGGGGTCATGACGACTTACCGCGCCATAGCAAACTCCGAATACCCCGAAGTATGAGCACGGG7490.11973999315771468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.11862092780110052No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG7420.11862092780110052Clontech Universal Primer Mix Long (96% over 25bp)
GTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACAGGATTTCACGTGTC7360.11766172892400267No Hit
CTCCCCTACCATGGCAAAATCACTTCTGCCATCCGCAGCTTCGGTTACTTGCTTGAGCCCCGTTACATCTTCCGC7260.1160630641288396No Hit
GTAGGGGAGCGTTCCGTAGGCCGCAGAAGGCAGCTCGTGAGGGCTGCTGGAGGTATCGGAAGTGCGAATGCTGAC7130.1139847998951276No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7120.11382493341561127Clontech Universal Primer Mix Long (96% over 26bp)
ATTCAAGCGTGTGGCACGAAGCGGGCCCTTGGGTCCGCCAAGCAACGGGCAGTGGTTCCAGGAAAAGCCTCTAAG7080.11318546749754606No Hit
GTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCCCAGGA6990.11174666918189928No Hit
GTCTTAGCCTTCCCACCTCGTTTCCCACTTAGCAAGTATTGGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTC6850.10950853846867095No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC6640.10615134239882851No Hit
CCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTACGGCGACAGGGCCTTTCACCCTGTTTAACGCTACT6410.1024744133699534No Hit
GGTTAGTGGAATGGTCTGGAAAGGCCGGCCGTAGCGGGTGACAGCCCCGTACACGAAAACCTGGCGGTGGTACTG6310.10087574857479034No Hit
ACTTAGATGTTTCAGTTCGCTGCGTTCGCCTCGCTGACCTATGTATTCAGCCAGCGATACCTCCTCAAAGAGAAG6310.10087574857479034No Hit
CCATTACAGTGCTCTACCCCCCCGAGCAATACACGAGGCACTACCTAAATAGTTTTCGGGGAGAACCAGCTATCT6290.10055601561575772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)