FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201935
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56572.801396488969223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35941.7797806224775299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21941.0864882264094882No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA20070.9938841706489713RNA PCR Primer, Index 46 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.7710401861985292No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.36496892564439054No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.31148636937628443No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.30108698343526386No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5560.2753361230098794RNA PCR Primer, Index 46 (96% over 30bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.22482481986777925No Hit
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3900.19313145319038305Illumina PCR Primer Index 8 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2570.12726867556391908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2270.11241240993388962No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.10646990368187784No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)