FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210866
Sequences flagged as poor quality0
Sequence length75
%GC23

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57942.7477165593315185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36291.7209981694535865No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24761.1742054195555471RNA PCR Primer, Index 46 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23861.1315242855652405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.8047764931283374No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.3381294281676515No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.31489192188404014No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6510.3087268691965514RNA PCR Primer, Index 46 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.2902317111340852No Hit
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5240.24849904678800758Illumina PCR Primer Index 8 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.21530260923999125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3650.17309571007179914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3050.14464162074492806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT2670.12662069750457638No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11571329659594244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT2320.11002247873056824No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.10765130461999563No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)