FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213026
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18920.8881544975730663RNA PCR Primer, Index 46 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.43938298611437104No Hit
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA6220.2919831382084816Illumina PCR Primer Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6020.28259461286415744RNA PCR Primer, Index 46 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.25630674190004976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.2149972303850234No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.13190878108775456No Hit
TATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2420.11360115666632242RNA PCR Primer, Index 46 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)