FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444059
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67031.5094841000857995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.8769105006316728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.780526911964401No Hit
TATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAGAACTG34580.7787253495594054No Hit
GTCCAGATAGGATCCGTCCACTTGCGTAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTAC26870.6050997727779417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.41323337664589616No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17650.3974697056021835RNA PCR Primer, Index 46 (96% over 30bp)
CCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACC16420.3697706836253741No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT16410.36954548832474965No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG14460.32563240470297866No Hit
GTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACG13100.2950058438180512No Hit
GTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAGATAGG13050.2938798673149289No Hit
AGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGG11540.2598753769206344No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTTAGGCTTGGGTACGTCG10930.24613846358254196No Hit
GTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAG10680.2405085810669303No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTAC10140.22834803483320912No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA9360.2107828013845007No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT8830.198847450451404No Hit
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8190.18443495121143813Illumina PCR Primer Index 8 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT8190.18443495121143813No Hit
TGCGTAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTACCCAAGCCTAAGAGCCAATCCTT8150.18353417000894026No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA8130.18308377940769133No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCG7870.17722870159145518No Hit
CTCTTAGGCTTGGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGG7850.17677831099020624No Hit
ACTAAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG7470.16822088956647654No Hit
GATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAG7430.16732010836397865No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGC7240.16304139765211378No Hit
GCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAGATAGGATCCGTCCACTTGCGTAAA7010.15786190573775105No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6880.15493436682963302RNA PCR Primer, Index 46 (96% over 30bp)
GGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCG6850.1542587809277596No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTCACCACCCATTTTGCGCTGCATTCC6520.1468273360071522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.14052186758966714No Hit
GGCTTGGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTA6200.13962108638716927No Hit
GGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGG5570.12543378244782788No Hit
TACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAG5400.12160546233721194No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTT5210.11732675162534707No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT5120.11529999391972688No Hit
GCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAGAACTGGTACGGACAAGGGGAATCTGAC5010.11282284561285776No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAG4990.11237245501160881No Hit
GATAGGATCCGTCCACTTGCGTAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTACCCAAG4660.10494101009100143No Hit
CTTCTGACTAAGGCCCGACGTACCCAAGCCTAAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGAC4620.10404022888850356No Hit
TGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAGATAGGATCCGTCCACTTG4600.10358983828725461No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC4450.10021190877788763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)