FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444059
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAGAACTG34430.7753474200500384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.7046360956539559No Hit
GTCCAGATAGGATCCGTCCACTTGCGTAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTAC25420.572446454187394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.3981452915040569No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT16290.36684314471725604No Hit
CCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACC15820.35625896558790615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.3159490067761266No Hit
GTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACG13690.3082923665548947No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG13510.30423885114365434No Hit
GTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAGATAGG12150.2736122902587269No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTTAGGCTTGGGTACGTCG11280.25402029910439833No Hit
AGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGG10870.24478729177879519No Hit
GTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAG10440.2351038938519431No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA10220.23014959723820483No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTAC9940.22384412882071975No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT9270.2087560436788805No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCG8470.19074041962892319No Hit
TGCGTAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTACCCAAGCCTAAGAGCCAATCCTT8070.18173260760394452No Hit
CTCTTAGGCTTGGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGG7850.17677831099020624No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGC7630.171824014376468No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA7610.17137362377521906No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT7370.16596893656023187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.16416737415523613No Hit
ACTAAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG7240.16304139765211378No Hit
GATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAG7040.15853749163962447No Hit
GGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGGACTGGTCGGTGGACGGCTTTGGCTGCTTTCGGGCG6760.1522320232221394No Hit
GCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAGATAGGATCCGTCCACTTGCGTAAA6460.14547616420340542No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTCACCACCCATTTTGCGCTGCATTCC6230.14029667228904266No Hit
GGCTTGGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTA6000.13511718037467993No Hit
TACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAGCAGCCAAAGCCGTCCACCGACCAGTCCAG5280.11890311872971836No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGACGCCCGAAAG5250.11822753282784496No Hit
GGGTACGTCGGGCCTTAGTCAGAAGCCATCGGACTGGACGGGAACTGTTTACGCAAGTGGACGGATCCTATCTGG5060.11394882211598009No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTT4950.11147167380911095No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT4850.1092197208028663No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC4750.10696776779662162No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTTAGTTGCTTGTTAAGCGTACGCCGGAC4650.10471581479037696No Hit
GCTTTGGCTGCTTTCGGGCGTCCGGCGTACGCTTAACAAGCAACTAAGAACTGGTACGGACAAGGGGAATCTGAC4650.10471581479037696No Hit
GGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTGTTGACGCAAT4460.1004371040785121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)