FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131458
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA101257.70207975170777RNA PCR Primer, Index 46 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA34812.6479940361179994Illumina PCR Primer Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA31402.3885955970728294RNA PCR Primer, Index 46 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13271.0094478844954282No Hit
TATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA11880.9037106908670449RNA PCR Primer, Index 46 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA11740.893060901580733RNA PCR Primer, Index 46 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.5591139375313788No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA6550.49825799875245325RNA PCR Primer, Index 46 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.4366413607387911No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC5230.397845699767226RNA PCR Primer, Index 46 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA5180.39404220359354314RNA PCR Primer, Index 46 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3920.29819410001673535TruSeq Adapter, Index 8 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA3560.2708089275662189RNA PCR Primer, Index 46 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG3470.26396263445358975RNA PCR Primer, Index 46 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.2624412359841166No Hit
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA3400.25863773981043375RNA PCR Primer, Index 46 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA2870.21832068036939556RNA PCR Primer, Index 46 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA2490.1894141094494059RNA PCR Primer, Index 46 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA2490.1894141094494059Illumina PCR Primer Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA2440.18561061327572306RNA PCR Primer, Index 46 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG2430.18484991404098647RNA PCR Primer, Index 46 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA2040.15518264388626027Illumina PCR Primer Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA2040.15518264388626027RNA PCR Primer, Index 46 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC1930.14681495230415798RNA PCR Primer, Index 46 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC1900.14453285459994827Illumina PCR Primer Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA1840.13996865919152884RNA PCR Primer, Index 46 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA1770.13464376454837285RNA PCR Primer, Index 46 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA1770.13464376454837285Illumina PCR Primer Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG1710.13007956913995344Illumina PCR Primer Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA1500.11410488521048548TruSeq Adapter, Index 8 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA1460.1110620882715392RNA PCR Primer, Index 46 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA1400.10649789286311978RNA PCR Primer, Index 46 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT1350.10269439668943693RNA PCR Primer, Index 46 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)