FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366223
Sequences flagged as poor quality0
Sequence length75
%GC25

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94042.567834352293548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51931.4179884933496805No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA50551.3803065345431609RNA PCR Primer, Index 46 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43761.1949003749081843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.6810058352424615No Hit
TTATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA19000.518809577770921Illumina PCR Primer Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA14760.40303312462625235RNA PCR Primer, Index 46 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.25503586612528434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT7250.19796681257048301No Hit
TATACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA7160.19550929351788393RNA PCR Primer, Index 46 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6810.18595227497999853No Hit
ACACATCTCCGAGCCCACGAGACCGTCTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA5770.15755427703885339RNA PCR Primer, Index 46 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT5740.15673510402132035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.15482370031374326No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.1354366055654615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.13461743254792846Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT4740.12942933677021926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT4680.12779099073515315No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.12069149124986688No Hit
GCGTTGATACCACTGCTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.11659562616220172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.11331893409206958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT3750.10239662719162915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)