FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224729
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51552.293873954852289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31511.4021332360309529No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA30511.3576351961696087RNA PCR Primer, Index 36 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.895745542408857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.6492264015770105No Hit
ATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA8790.3911377703812147RNA PCR Primer, Index 36 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7320.32572565178503887RNA PCR Primer, Index 38 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.26787819996529155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.23405968967067No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.23005486608314907No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.16909255147310762No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG3530.15707808071054472No Hit
ACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3240.14417364915075492RNA PCR Primer, Index 36 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2310.10279047207970489RNA PCR Primer, Index 36 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)