FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203442
Sequences flagged as poor quality0
Sequence length75
%GC24

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56222.763441177338013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34261.6840180493703367No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29751.4623332448560278RNA PCR Primer, Index 36 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22651.113339428436606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.7903972631020143No Hit
ATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9030.44386114961512374RNA PCR Primer, Index 36 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7680.37750317043678294RNA PCR Primer, Index 38 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.3637400340146086No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.3155690565369983No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.2875512431061433No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.2320071568309395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3230.15876760944151158No Hit
ACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2830.13910598598126248RNA PCR Primer, Index 36 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2740.13468212070270644RNA PCR Primer, Index 36 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2500.12288514662655696No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10715584785835766No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10322352316630783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)