FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002153904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182774
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37712.063203737949599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22571.2348583496558592No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18511.0127260988980928RNA PCR Primer, Index 40 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.8743037850022432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.5865166818037577No Hit
ATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5150.28176874172475297RNA PCR Primer, Index 40 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.2741090089400024No Hit
TTATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4210.2303391073128563TruSeq Adapter, Index 8 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.22705636469082033No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.2172081368247125No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.17070261634586978No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1960.10723625898650792RNA PCR Primer, Index 40 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1900.10395351636447198No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)