FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249624
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57682.310675255584399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32691.3095695926673718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26361.0559882062622183No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18520.7419158414255039Illumina PCR Primer Index 7 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.6285453321795981No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.3136717623305451No Hit
ATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6380.25558439893599977Illumina PCR Primer Index 7 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.2359548761337051No Hit
TTATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5790.2319488510720123Illumina PCR Primer Index 7 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.2055090856648399No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.15943979745537287No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)