FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002153936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002153936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252606
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46561.843186622645543No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA31751.2568980942653776Illumina PCR Primer Index 7 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28091.1120084241862822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.8903193114969556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.5300745033768002No Hit
ATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9920.39270642819252116Illumina PCR Primer Index 7 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7320.289779340158191Illumina PCR Primer Index 7 (96% over 30bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.2307942012462095No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.1884357457859275No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.18685225212386086No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.13697220176876243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3390.13420108786014584No Hit
ACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2910.11519916391534643Illumina PCR Primer Index 7 (96% over 30bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2720.10767756902053No Hit
TATACACATCTCCGAGCCCACGAGACATGAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA2640.10451058169639675Illumina PCR Primer Index 7 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)