FastQCFastQC Report
Wed 8 May 2024
EGAF00002167534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11955857
Total Bases255.6 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8502297.111401549884714No Hit
TCAGTGCACTACAGAACTTTGT4957364.146386160356384No Hit
CGCGACCTCAGATCAGA3040712.543280669884225No Hit
TCCTGTACTGAGCTGCCCCGAG1929811.6141126478846308No Hit
TGAGGTAGTAGGTTGTATAGTT1759311.4715047194023816No Hit
TGAGGTAGTAGGTTGTATAGT1755061.467949976317047No Hit
TTCAAGTAATCCAGGATAGGCT1706301.4271666180015368No Hit
TCCTGTACTGAGCTGCCCCGA1438201.2029250600772492No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1217771.0185551734183504No Hit
TGAGGTAGTAGATTGTATAGTT1145250.9578987102304753No Hit
TTCACAGTGGCTAAGTTCCG1081340.9044437383284193No Hit
TGAGGTAGTAGATTGTATAGT968890.8103894183411527No Hit
TGGCTCAGTTCAGCAGGAAC950430.7949492871987344No Hit
CGCGACCTCAGATCAGACGT910460.7615179739938341No Hit
TATTGCACTTGTCCCGGCCTGT879070.7352630597706212No Hit
TGAGGTAGTAGGTTGTGTGGT872250.7295587426313312No Hit
TCGTACCGTGAGTAATAATGCG857940.717589713560475No Hit
TGAGGTAGTAGGTTGTGTGGTT850050.7109904375738184No Hit
TGAGGTAGTAGGTTGTATAG816900.6832634415082081No Hit
TGAGGGGCAGAGAGCGAGACTTT794180.6642602031790779No Hit
TAGCTTATCAGACTGATGTTGAC793080.6633401520275795No Hit
AAGCTGCCAGTTGAAGAACTGT780870.6531275842459474No Hit
AAACCGTTACCATTACTGAG762420.6376958172049063No Hit
TAGCTTATCAGACTGATGTTGA759200.6350025765614293No Hit
AAAAGCTGGGTTGAGAGGGCGA758170.6341410741195718No Hit
CATTGCACTTGTCTCGGTCTGA738690.617847804636673No Hit
CCCATAAAGTAGAAAGCACT727590.6085636521079166No Hit
TGGAGTGTGACAATGGTGTTT560450.46876606168842605No Hit
TGAGGGGCAGAGAGCGAGACT543440.4545387252457101No Hit
TCCTGTACTGAGCTGCCCCGAGA532990.44579823930647544No Hit
TGAGGTAGTAGGTTGTGTGG524010.43828727626969777No Hit
AAACCGTTACCATTACTGAGC515160.43088504655082444No Hit
CGCGACCTCAGATC503380.4210321351284145No Hit
TGGCTCAGTTCAGCAGGAACAG502600.4203797352209883No Hit
TGAGGTAGTAGTTTGTACAGT478630.4003309842197008No Hit
TGAGGTAGTAGTTTGTGCTGTT451150.3773464336349958No Hit
AAATGGATTTTTGGAGCAGGGAGA443180.37068024483732115No Hit
TAGCTTATCAGACTGATGTTG424460.35502264705909414No Hit
TCCTGTACTGAGCTGCCCCG418350.3499121811175895No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT408100.3413389772059No Hit
TGGAGAGAAAGGCAGTTCCTGA369340.3089197202676479No Hit
TGAGGTAGTAGTTTGTACAGTT356630.29828894741715295No Hit
CTAGACTGAAGCTCCTTGAGG351790.29424072235056004No Hit
TGAGATGAAGCACTGTAGCT338500.28312483162018415No Hit
TGAGGTAGTAGATTGTATAG328290.27458508411400373No Hit
TCAGTGCACTACAGAACTTTGC319310.267074121077226No Hit
TGTAAACATCCCCGACTGGAAG315960.26427214711584457No Hit
CGTGTATTTGACAAGCTGAGTTG308930.2583921838476322No Hit
AAATGGATTTTTGGAGCAGGGA297380.24873164675689916No Hit
TGGCTCAGTTCAGCAGGAACA280710.23478868976101003No Hit
AAATGGATTTTTGGAGCAGGGAG271540.2271188087980644No Hit
TCCTGTACTGAGCTGCCCCGAGT268160.22429174253255119No Hit
TGAGATGAAGCACTGTAGCTC266580.22297021451494442No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT264650.22135594294913366No Hit
AAACCGTTACCATTACTG263330.22025188156733558No Hit
AAAAGCTGGGTTGAGAGGGCG260470.21785974857343976No Hit
TGAGAACTGAATTCCATGGGTT257880.21569344631672993No Hit
TTCAAGTAATTCAGGATAGGT253640.21214706733277255No Hit
CATTGCACTTGTCTCGGTCTG252460.2111601033702561No Hit
CGCGACCTCAGATCAGACG221060.1848968250456659No Hit
TGAGGGGCAGAGAGCGAGACTT219570.18365057394045445No Hit
AAATGGATTTTTGGAGCAGGG211220.176666549290444No Hit
AGAGGTAGTAGGTTGCATAGT202510.16938141699085227No Hit
CGCGACCTCAGATCAGAC201790.1687792016916897No Hit
ATTTTTGGAGCAGGGAGA200530.16772532491815517No Hit
AGAGGTAGTAGGTTGCATAGTT197200.16494007915952824No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC196630.16446332538102457No Hit
AAATGGATTTTTGGAAATAGGAGA190950.159712515798742No Hit
CAACGGAATCCCAAAAGCAGCTG186530.15601558298999396No Hit
TGTAAACATCCCCGACTGGAAGC183980.15388273713879314No Hit
GGCTGGTCCGATGGTAGTG183110.1531550603189717No Hit
CAACGGAATCCCAAAAGCAGCT183020.15307978340657638No Hit
TGAGGTAGTAGGTTGTAT180320.1508214760347167No Hit
ACGGCCCTGGCGGAGC179240.14991815308597284No Hit
TGTAAACATCCCCGACTGGAAGCT175130.1464805074199198No Hit
AAAAGCTGGGTTGAGAGGGCGAA173780.14535135373398997No Hit
AGAAGCCGGCGGGAGC173700.14528444092297188No Hit
AAATGGATTTTTGGAGCAGGGAGAT169240.14155405170871482No Hit
TGAGGTAGTAGTTTGTGCTGT162240.13569918074463422No Hit
TGGAGAGAAAGGCAGTTCCTG161600.13516387825648968No Hit
TAGCAGCACGTAAATATTGGC161400.13499659622894453No Hit
TTTTTGGAGCAGGGAGA157160.13145021724498712No Hit
TACAGTACTGTGATAACTGAAG156760.1311156531898968No Hit
AACATTCAACGCTGTCGGTGA156660.13103201217612423No Hit
TTCACAGTGGCTAAGTTCCGA156070.130538530194866No Hit
TTTGGAGCAGGGAGA154040.12884061761528262No Hit
TGTAAACATCCTTGACTGGAAGCT153140.1280878484913294No Hit
TGTCAGTTTGTCAAATACCCCAA151490.12670777176408182No Hit
TGGACGGTGTGAGGCC148970.1246000182170128No Hit
TAGCAGCACGTAAATATTGGCG148400.1241232644385091No Hit
AGCTCGGTCTGAGGCCCCTCAGT148130.12389743370132313No Hit
AAACCGTTACCATTACTGAGTT147620.12347086453108297No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG146060.1221660647162307No Hit
AGAGGTAGTAGGTTGCATAG142570.11924699333556767No Hit
GAAGAGGGGGACGG139090.11633628605628188No Hit
AGGAAACTCTGGTG138990.11625264504250929No Hit
TGTCAGTTTGTCAAATACCCCA137080.11465510167945302No Hit
AAAAGCTGGGTTGAGAGGGCGAT135610.11342557877699608No Hit
TGAGGTAGTAGGTTGTGTGGTTT133790.1119033123263351No Hit
TTCAAGTAATCCAGGATAGGCC133590.11173603029878995No Hit
GGCTGGTCCGATGGTAGT131610.11007993822609285No Hit
TCGTACCGTGAGTAATAATGC130230.10892569223603125No Hit
AAGCTGCCAGTTGAAGAACT130220.10891732813465399No Hit
TGGATTTTTGGAGCAGGGAGA130130.10884205122225867No Hit
TCAGTGCATCACAGAACTTTGT124010.10372322117937678No Hit
TTCAAGTAATTCAGGATAGGTT123510.10330501611051388No Hit
TGAAAAGAACTTTGAAGAGAG123370.10318791869123226No Hit
AGCAGCATTGTACAGGGCTATGA122730.10265261620308774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC122710.10263588800033324No Hit
TGTAAACATCCCCGACTGGAA120050.1004110370339826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)