FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10997048
Sequences flagged as poor quality0
Sequence length14-50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT3936133.579260543374913No Hit
TCAGTGCACTACAGAACTTTGT2204772.004874399020537No Hit
TTCAAGTAATCCAGGATAGGCT826130.7512288752399735No Hit
TGAGGTAGTAGGTTGTATAGT824860.7500740198642399No Hit
CGCTACCTGCGGGACTGT815530.7415899248598351No Hit
TGAGGTAGTAGGTTGTATAGTT721400.6559942268143233No Hit
TGGAGTGTGACAATGGTGTTT659870.6000428478624446No Hit
AAACCGTTACCATTACTGAGC634050.5765638196723339No Hit
TATTGCACTTGTCCCGGCCTGT561250.5103642359294968No Hit
TGAGGTAGTAGGTTGTGTGGT544800.49540567614145176No Hit
TCCTGTACTGAGCTGCCCCGAG526910.4791376740376145No Hit
TGAGGTAGTAGATTGTATAGTT510030.46378810022471484No Hit
GCCTTGAGGGCTGTTGTGGAG503840.4581593169366906No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC490830.44632886934748306No Hit
TGAGGTAGTAGATTGTATAGT488980.4446465997056665No Hit
TGAGGTAGTAGGTTGTGTGGTT474680.43164311004189493No Hit
AAGCTGCCAGTTGAAGAACTGT459030.41741201820706797No Hit
CGCGACCTCAGATCAGA448720.4080367749599711No Hit
TGAGGGGCAGAGAGCGAGACTTT446860.4063454119687392No Hit
GCCTTGAGGGCTGTTGTGGAGA435700.39619723402134827No Hit
GGCCTTGAGGGCTGTTGTGGAGA432090.3929145348824521No Hit
TCCTGTACTGAGCTGCCCCGA415710.37801962854031373No Hit
CTACCTGCGGGACTGTGC401320.3649342987318051No Hit
CCCATAAAGTAGAAAGCACT370390.33680856899051453No Hit
TCAGTGCACTACAGAACTTTGC366900.33363499004460107No Hit
CTACCTGCGGGACTGTG356910.32455073397879136No Hit
GGCCTTGAGGGCTGTTGTGGAG343720.31255660610010977No Hit
TGAGGGGCAGAGAGCGAGACT332330.3021992811161686No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT307920.2800024151936047No Hit
CGGGATCTTCCCCTCGGA307070.2792294804933106No Hit
TGAGGGCTGTTGTGGAGA267320.24308341656779164No Hit
TGGCTCAGTTCAGCAGGAAC265710.24161938731194046No Hit
TTCACAGTGGCTAAGTTCCG263950.24001895781486085No Hit
TGAGGTAGTAGTTTGTACAGT251770.22894325822711692No Hit
GCTACCTGCGGGACTGT250420.2277156560560616No Hit
ACGCTACCTGCGGGACTGT248310.22579695932944915No Hit
CGCTACCTGCGGGACTGTG247830.22536047855751837No Hit
CGCGACCTCAGATCAGACGT244440.2222778331057571No Hit
TCGTACCGTGAGTAATAATGCG241500.21960438837768098No Hit
TAGCTTATCAGACTGATGTTGA238260.21665814316714815No Hit
TGAGGGCTGTTGTGGAG237620.21607616880457373No Hit
TACCTGCGGGACTGTGC236430.21499406022416198No Hit
CGCCGTCGGGAGGTCCCGGA231000.21005637149169487No Hit
TGAGGTAGTAGTTTGTGCTGTT219850.19991728689371913No Hit
GCTACCTGCGGGACTGTG212260.19301543468756344No Hit
CGCTACCTGCGGGACTGTGC211200.1920515396495496No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT207200.18841419988345964No Hit
TGAGATGAAGCACTGTAGCT206580.18785041221971568No Hit
GCCTTGAGGGCTGTTGTG201150.18291272348724857No Hit
CGGGATCTTCCCCTCGGAC198160.18019381201209633No Hit
TCCTGTACTGAGCTGCCCCGAGA190890.17358294698722784No Hit
TGTAGGTAAGGGAA190630.173346519902432No Hit
AAAAGCTGGGTTGAGAGGGCGA186440.16953640649745277No Hit
TACCTGCGGGACTGTG184010.1673267225895531No Hit
CTACCTGCGGGACTGT179460.1631892486056258No Hit
TGAGGTAGTAGGTTGTATAG175900.15995201621380575No Hit
TAGCTTATCAGACTGATGTTGAC175120.15924273495941818No Hit
CATTGCACTTGTCTCGGTCTGA173710.1579605726918715No Hit
TGAGGTAGTAGGTTGTGTGG171360.15582363557929366No Hit
TCCTGTACTGAGCTGCCCCGAGT165470.15046765277372617No Hit
AAACCGTTACCATTACTGAG162790.1480306351304459No Hit
TTCAAGTAATCCAGGATAGGCC162210.14750322086436288No Hit
CGCTACCTGCGGGACTGC156290.14211995801054975No Hit
CTACCTGCGGGACTGTGCT155450.14135611665967085No Hit
CTACCTGCGGGACTGTGT155180.14111059622545977No Hit
GACGGGCAGCTTCCGGGAAACT151700.1379461106289615No Hit
CGCTACCTGCGGGACTG151460.13772787024299613No Hit
AGGAGTGGAGCCTGCGGCTTA148710.13522719915380926No Hit
CGCTACCTGCGGGACT148350.13489983857486118No Hit
TAGCTTATCAGACTGATGTTG148330.13488165187603074No Hit
TGAGGGCTGTTGTGGA148190.13475434498421757No Hit
TGAGAACTGAATTCCATGGGTT146860.13354492951199268No Hit
CCGTCGGGAGGTCCC143250.13026223037309648No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC142180.12928924198566744No Hit
GCCTTGAGGGCTGTTGTGG139030.12642483691987158No Hit
GCTACCTGCGGGACTGTGC137520.1250517411581726No Hit
TGGCTCAGTTCAGCAGGAACAG137400.12494262096518992No Hit
GCCGTCGGGAGGTCCCGGA136150.12380595228828682No Hit
GGCCTTGAGGGCTGTTGTGGAGAAG133890.12175085532044601No Hit
TGAGGTAGTAGGTTGTATAGC133400.12130528119909997No Hit
GCTACCTGCGGGACTG132170.1201867992210273No Hit
GCCTTGAGGGCTGTTGTGGA131710.11976850514792696No Hit
TGAGGTAGTAGTTTGTACAGTT130750.11889554360406539No Hit
AGGAGTGGAGCCTGCGGCTT128840.11715871386575744No Hit
CGTGAAACCGTTTAGGGGT128370.11673132644324186No Hit
CTAGACTGAAGCTCCTTGAGG127450.11589473829704117No Hit
TGAGGTAGTAGGTTGTATAGTC124130.1128757462911865No Hit
TTCAAGTAATTCAGGATAGGT122260.11117528995053946No Hit
TGTAAACATCCCCGACTGGAAGCT120840.10988403433357752No Hit
ACCTGCGGGACTGTGC117550.10689232237596853No Hit
TATTGCACTTGTCCCGGCCTGC112950.1027093816449651No Hit
ATGTAGGTAAGGGAA111120.10104529870197893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)