FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002167536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9720074
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5695615.8596364595578185No Hit
TCAGTGCACTACAGAACTTTGT3186113.277865991555208No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2994333.0805629669074537No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2640322.716357920731879No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2273102.3385624430431293No Hit
TTCAAGTAATCCAGGATAGGCT1425451.4665011809580872No Hit
TGAGGTAGTAGGTTGTATAGT1233941.2694759319733575No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1176261.2101348199612472No Hit
AAACCGTTACCATTACTGAGC1172111.205865305140681No Hit
TATTGCACTTGTCCCGGCCTGT1144911.1778819790878134No Hit
TCCTGTACTGAGCTGCCCCGAG1076141.1071314889166481No Hit
TGAGGTAGTAGTTTGTGCTGTT1022591.0520393157500652No Hit
TGAGGTAGTAGGTTGTATAGTT989211.017698013410186No Hit
TGAGGGGCAGAGAGCGAGACTTT966830.9946734973416869No Hit
TGAGGTAGTAGGTTGTGTGGT884690.9101679678570349No Hit
TAGCTTATCAGACTGATGTTGAC881160.9065363082626737No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT878390.9036865357197897No Hit
TGAGGTAGTAGGTTGTGTGGTT821910.8455799822100121No Hit
TGAGGTAGTAGATTGTATAGTT802260.8253640867343192No Hit
TGAGGTAGTAGATTGTATAGT784060.8066399494489446No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC780910.8033992333803219No Hit
TCCTGTACTGAGCTGCCCCGA775390.7977202642695931No Hit
AAGCTGCCAGTTGAAGAACTGT701980.7221961478894091No Hit
TCAGTGCACTACAGAACTTTGC697800.7178957691062846No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC612610.6302524034282043No Hit
TGAGGTAGTAGTTTGTACAGT555860.5718680742554018No Hit
TGAGGGGCAGAGAGCGAGACT523710.5387921943804131No Hit
CATTGCACTTGTCTCGGTCTGA478010.49177609141658796No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC476020.4897287819002201No Hit
TAGCTTATCAGACTGATGTTGA414910.4268588901689432No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT409100.4208815694201505No Hit
TTCAAGTAATCCAGGATAGGCC358360.3686803207465293No Hit
TCCTGTACTGAGCTGCCCCGAGT352600.3627544399353338No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT345660.35561457659684487No Hit
CGCGACCTCAGATCAGA333560.34316611169832656No Hit
CCCATAAAGTAGAAAGCACT313930.32297079219767255No Hit
TCCTGTACTGAGCTGCCCCGAGA311850.32083089079362975No Hit
TGGCTCAGTTCAGCAGGAAC301930.3106252071743487No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT292540.30096478689359774No Hit
TGGAGTGTGACAATGGTGTTT292410.30083104305584507No Hit
TATTGCACTTGTCCCGGCCTGC289420.2977549347875335No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT281660.28977145647245073No Hit
CGCGACCTCAGATCAGACGT277120.28510071013862653No Hit
TGTAAACATCCCCGACTGGAAGCT274330.2822303616207037No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT266140.27380449984228517No Hit
TCCTGTACTGAGCTGCCCCG261890.2694321051465246No Hit
TGAGGTAGTAGGTTGTATAGC260620.26812553073155615No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG259670.2671481719172097No Hit
TCGTACCGTGAGTAATAATGCG255280.262631745396177No Hit
AAACCGTTACCATTACTGAG247370.25449394726830266No Hit
TGAGGTAGTAGTTTGTACAGTT237040.2438664561607247No Hit
GCATTGGTGGTTCAGTGGTAGAATT236370.24317716099692246No Hit
TGAGGTAGTAGTTTGTGCTGTC231410.23807431918728192No Hit
TGAGGTAGTAGGTTGTGTGG226440.23296118939012192No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT224140.23059495226065152No Hit
TGAGGTAGTAGGTTGTATAGTC222870.22928837784568307No Hit
TGTAAACATCCTTGACTGGAAGCT216900.22314644929657942No Hit
AAACCGTTACCATTACTGAGTT209670.21570823432002678No Hit
TGAGGTAGTAGTTTGTGCTGT208850.21486461934343298No Hit
TTCACAGTGGCTAAGTTCCG206720.21267327800179298No Hit
TGAGGTAGTAGGTTGTATAG202870.20871240280680992No Hit
TGAGGTAGTAGGTTGTGTGGC201870.20768360405486624No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC200550.2063255897023006No Hit
TGAGGTAGTAGGTTGTGTGGTC198810.20453547987391865No Hit
AAAAGCTGGGTTGAGAGGGCGA191880.1974059045229491No Hit
TAGCTTATCAGACTGATGTTG189640.1951013953185953No Hit
TAGCTTATCAGACTGATGTTGAT189100.19454584399254574No Hit
TGAGATGAAGCACTGTAGCTC188460.1938874127913018No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC187570.19297178190207193No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA181710.1869430212156821No Hit
TGAGGGGCAGAGAGCGAGACTT177780.18289984212054353No Hit
TGAGGTAGTAGATTGTATAGTC177260.1823648667695328No Hit
TGAGAACTGAATTCCATGGGTT170630.17554393104414637No Hit
TGAGGGGCAGAGAGCGAGACTTC170400.17530730733119934No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC168100.1729410702017289No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA166550.17134643213621625No Hit
CATTGCACTTGTCTCGGTCTG164070.16879501123139598No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT163230.1679308202797633No Hit
TGAGGTAGTAGATTGTATAGC162220.1668917335403002No Hit
AGCTCGGTCTGAGGCCCCTCAGT160760.16538968736246246No Hit
TGAGATGAAGCACTGTAGCT157870.16241645896934528No Hit
CGCGACCTCAGATC156900.16141852417995994No Hit
AAGCTGCCAGTTGAAGAACTGC156510.16101729266670192No Hit
TGTAAACATCCCCGACTGGAAG156430.16093498876654644No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC151260.15561609921899772No Hit
TTCAAGTAATTCAGGATAGGT148100.15236509516285576No Hit
CTAGACTGAAGCTCCTTGAGG141110.1451737918867696No Hit
CAACGGAATCCCAAAAGCAGCTG137140.14108946084155327No Hit
TGAGGTAGTAGGTTGTAT125780.1294023070194733No Hit
TACAGTATAGATGATGTACT123010.12655253447658937No Hit
TCCTGTACTGAGCTGCCCCGAA122090.1256060396248012No Hit
TGAGGGGCAGAGAGCGAGACC121730.1252356720741015No Hit
TGAGGTAGTAGTTTGTACAGC116840.12020484617709701No Hit
TTCAAGTAATCCAGGATAGGCA115710.11904230358740069No Hit
TCAGTGCATCACAGAACTTTGT115090.1184044483611956No Hit
TGAGGTAGTAGATTGTAT109930.11309584680116633No Hit
TGTAAACATCCCCGACTGGAAGC109740.11290037503829703No Hit
TGGCTCAGTTCAGCAGGAACAG107360.11045183400867113No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT106830.10990657067014098No Hit
TACAGTACTGTGATAACTGAAG105890.10893949984331396No Hit
CAACGGAATCCCAAAAGCAGCT105820.1088674839306779No Hit
TAGCTTATCAGACTGATGTTGACT105660.1087028761303669No Hit
AAACCGTTACCATTACTG104360.10736543775284016No Hit
TGAGGTAGTAGATTGTATAG104210.10721111794004859No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG103350.10632635101337706No Hit
TGAGGTAGTAGGTTGTGTGGTTT100020.10290045116940467No Hit
AGAGGTAGTAGGTTGCATAGTT98920.10176877254226666No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGT98500.10133667706645033No Hit
AAGCTGCCAGTTGAAGAACT97440.10024615038939004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)