FastQCFastQC Report
Thu 9 May 2024
EGAF00002167537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9182132
Total Bases206.6 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6584697.1711994556384075No Hit
TCAGTGCACTACAGAACTTTGT2157582.3497592933754383No Hit
CGCGACCTCAGATCAGA2048832.2313227472661032No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1420411.5469283168658432No Hit
TCCTGTACTGAGCTGCCCCGAG1383871.5071336373731068No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1344211.4639410542126818No Hit
TTCAAGTAATCCAGGATAGGCT1083451.1799547207554848No Hit
TCCTGTACTGAGCTGCCCCGA1028921.1205676415891213No Hit
AAACCGTTACCATTACTGAGC982201.0696862123088624No Hit
TATTGCACTTGTCCCGGCCTGT943491.027528247252381No Hit
TGAGGGGCAGAGAGCGAGACTTT837110.9116728010444632No Hit
TGAGGTAGTAGGTTGTATAGTT794370.8651258770838842No Hit
AAGCTGCCAGTTGAAGAACTGT772720.8415474750308534No Hit
TGAGGTAGTAGGTTGTATAGT675790.7359837562779538No Hit
TAGCTTATCAGACTGATGTTGAC668980.7285671780802105No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC614340.6690603010281272No Hit
TGAGGTAGTAGATTGTATAGTT597870.65112329032081No Hit
AAACCGTTACCATTACTGAG560380.6102939927241299No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT544170.5926401406557866No Hit
TGAGGGGCAGAGAGCGAGACT515210.5611006245608319No Hit
CATTGCACTTGTCTCGGTCTGA508400.5536840463630887No Hit
TGGAGTGTGACAATGGTGTTT495630.5397766009027097No Hit
TGAGGTAGTAGGTTGTGTGGT469480.5112973762520513No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG468740.5104914632026636No Hit
TGAGGTAGTAGGTTGTGTGGTT462600.5038045630361228No Hit
TGAGGTAGTAGATTGTATAGT444170.4837329718196166No Hit
CGCGACCTCAGATC428660.46684146993312664No Hit
TGGCTCAGTTCAGCAGGAAC423450.4611674064367622No Hit
TGAGGTAGTAGTTTGTGCTGTT415400.45240037934545047No Hit
TCGTACCGTGAGTAATAATGCG404850.4409106730332345No Hit
TAGCTTATCAGACTGATGTTGA386540.4209697704193318No Hit
AAAAGCTGGGTTGAGAGGGCGA374880.40827119453303434No Hit
TCCTGTACTGAGCTGCCCCGAGA360000.3920658078102123No Hit
CGCGACCTCAGATCAGACGT356570.38833029191913165No Hit
TCAGTGCACTACAGAACTTTGC346580.37745046575239827No Hit
TCCTGTACTGAGCTGCCCCG340010.37029526475986185No Hit
CCCATAAAGTAGAAAGCACT316150.3443100142755517No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT303700.3307510717554485No Hit
TGAGGTAGTAGTTTGTACAGT261010.2842586013792875No Hit
TGAGGTAGTAGGTTGTATAG259350.28245074237660706No Hit
TGTAAACATCCCCGACTGGAAG253970.2765915366932212No Hit
TTCACAGTGGCTAAGTTCCG252250.274718333389239No Hit
CTAGACTGAAGCTCCTTGAGG249600.2718322934150805No Hit
TGAGGTAGTAGGTTGTGTGG237780.2589594660586452No Hit
TCCTGTACTGAGCTGCCCCGAGT233260.25403686202725034No Hit
AAACCGTTACCATTACTG227590.24786182555423947No Hit
TGAGGGGCAGAGAGCGAGACTT226970.24718660110745524No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT222630.24246002997996546No Hit
TGAGAACTGAATTCCATGGGTT217860.23726515802648013No Hit
TAGCTTATCAGACTGATGTTG214050.23311579489382206No Hit
CAACGGAATCCCAAAAGCAGCTG210260.2289882131949312No Hit
TGAAAAGAACTTTGAAGAGAG199760.21755296046713332No Hit
TGGCTCAGTTCAGCAGGAACAG198870.21658368666449143No Hit
TGAGATGAAGCACTGTAGCTC195760.21319667371368653No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC195720.2131531108461521No Hit
TTCAAGTAATCCAGGATAGGCC194400.2117155362175146No Hit
TGTAAACATCCCCGACTGGAAGCT180730.19682792623761017No Hit
CATTGCACTTGTCTCGGTCTG177300.19309241034652955No Hit
AAACCGTTACCATTACTGAGTT173740.18921531513596188No Hit
TGAGGTAGTAGTTTGTACAGTT168190.18317096726555446No Hit
GGCTGGTCCGATGGTAGTG166260.18106905890701638No Hit
CGCGACCTCAGATCAGAC165940.18072055596674064No Hit
AGCTCGGTCTGAGGCCCCTCAGT161060.17540588612753552No Hit
TATTGCACTTGTCCCGGCCTGC158420.17253073687026063No Hit
GGCTGGTCCGATGGTAGT155230.1690565981843868No Hit
GCATTGGTGGTTCAGTGGTAGAATT151370.16485278146731064No Hit
TGTAAACATCCCCGACTGGAAGC149630.1629577967295613No Hit
TGAGGTAGTAGATTGTATAG142660.15536696706168024No Hit
GGCTGGTCCGATGGTAGTGGGT141140.15371157809537045No Hit
AGGAAACTCTGGTG140780.15331951228756022No Hit
CGCGACCTCAGATCAGACG138420.15074930310302662No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT136040.14815731248472577No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC132890.1447267366663864No Hit
TATTGCACTTGTCCCGGCCTG132750.14457426663001577No Hit
AAAAGCTGGGTTGAGAGGGCG131870.14361588354425747No Hit
TGAGGTAGTAGTTTGTGCTGT129900.1414704123181849No Hit
GGCTGGTCCGATGGTAGTGGGTT129610.14115458152856003No Hit
AAGCTGCCAGTTGAAGAACTGC127450.13880218668169877No Hit
TGAGATGAAGCACTGTAGCT125010.1361448517620962No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC121080.13186480002683473No Hit
AACATTCAACGCTGTCGGTGA116790.12719268248376303No Hit
TGAGGTAGTAGGTTGTATAGTC115370.12564620068628943No Hit
AAGCTGCCAGTTGAAGAACT111740.12169287045753643No Hit
GGCTGGTCCGATGGTAGTGGGTTATC111110.12100675529386858No Hit
AACCCGTAGATCCGATCTTGT108760.11844743682621857No Hit
TGAGGTAGTAGGTTGTATAGC106610.11610593269624091No Hit
TAGCAGCACGTAAATATTGGCG106520.11600791624428837No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG106230.11569208545466347No Hit
CAACGGAATCCCAAAAGCAGCT104480.1137862100000305No Hit
TCCTGTACTGAGCTGCCCCGAA103820.11306742268571177No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC99110.10793789503352816No Hit
TGGAGTGTGACAATGGTGTTTG98730.10752404779195072No Hit
GGCTGGTCCGATGGTAGTGGG97070.1057161887892703No Hit
TACAGTACTGTGATAACTGAAG96650.10525877868015837No Hit
TGTAAACATCCTTGACTGGAAGCT95860.10439841204635263No Hit
CGTGTATTTGACAAGCTGAGTTG94040.10241630157353435No Hit
CTAGACTGAAGCTCCTTGAGGA92480.10071734973969008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)