FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002167538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792790
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8369118.546195721546159No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2632412.688110334235698No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2527682.581164305575837No Hit
TCAGTGCACTACAGAACTTTGT2331782.381119170328374No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1835361.8741951987125223No Hit
AAACCGTTACCATTACTGAGC1828391.8670777173818698No Hit
TTCAAGTAATCCAGGATAGGCT1651461.6864039768033419No Hit
TATTGCACTTGTCCCGGCCTGT1548921.5816942873277178No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1070871.0935290147138863No Hit
TCCTGTACTGAGCTGCCCCGAG1046751.0688986489039385No Hit
TAGCTTATCAGACTGATGTTGAC877950.8965269346120972No Hit
AAGCTGCCAGTTGAAGAACTGT810720.8278743851343693No Hit
TGAGGTAGTAGGTTGTATAGT808610.8257197387057211No Hit
TGAGGTAGTAGTTTGTGCTGTT757760.7737937809347489No Hit
TGAGGTAGTAGGTTGTATAGTT742990.7587112559342128No Hit
TCCTGTACTGAGCTGCCCCGA707160.7221231130249909No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC664160.6782132568961451No Hit
TGAGGTAGTAGATTGTATAGTT660950.6749353350781544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC625770.6390109458080894No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT605200.6180056960273834No Hit
TGAGGGGCAGAGAGCGAGACTTT579660.5919252838057387No Hit
TGAGGTAGTAGATTGTATAGT572740.584858860447329No Hit
TGAGGTAGTAGTTTGTACAGT544990.5565216858525507No Hit
TCAGTGCACTACAGAACTTTGC538430.5498228798942896No Hit
CATTGCACTTGTCTCGGTCTGA499140.5097015253058628No Hit
CGCGACCTCAGATCAGA494710.5051777889651469No Hit
AAACCGTTACCATTACTGAG477210.48730749868015144No Hit
TAGCTTATCAGACTGATGTTGA467380.47726950133720825No Hit
AAACCGTTACCATTACTGAGTT465950.475809243331063No Hit
TTCAAGTAATCCAGGATAGGCC431240.44036479900008074No Hit
CCCATAAAGTAGAAAGCACT429720.43881263664389825No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC424120.43309414375269967No Hit
TATTGCACTTGTCCCGGCCTGC423640.4326039872191684No Hit
TGAGGTAGTAGGTTGTGTGGT405820.4144069259118188No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT400590.4090662620152173No Hit
TGAGGTAGTAGGTTGTGTGGTT387070.39526018632075227No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT384190.3923192471195645No Hit
CGCGACCTCAGATCAGACGT336420.34353846043875136No Hit
TGAGGGGCAGAGAGCGAGACT335690.34279301404400586No Hit
TGTAAACATCCTTGACTGGAAGCT319480.32624001944287584No Hit
TGTAAACATCCCCGACTGGAAGCT313520.3201539091515288No Hit
TGAGGTAGTAGTTTGTACAGTT302870.3092785610638031No Hit
TCCTGTACTGAGCTGCCCCGAGT299880.306225294323681No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG299290.30562281025121546No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT296340.30261038988888767No Hit
TCCTGTACTGAGCTGCCCCGAGA288620.2947270389745925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT282060.2880282330163314No Hit
GCATTGGTGGTTCAGTGGTAGAATT270530.2762542646171316No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT269190.2748859109610234No Hit
TGGCTCAGTTCAGCAGGAAC268490.2741710993496235No Hit
TCCTGTACTGAGCTGCCCCG242590.24772306972783037No Hit
TCGTACCGTGAGTAATAATGCG241120.24622196534389074No Hit
TAGCTTATCAGACTGATGTTGAT232600.23752168687371017No Hit
TGAGAACTGAATTCCATGGGTT223600.22833125186999823No Hit
TGAGGTAGTAGTTTGTGCTGT217220.22181625461181134No Hit
CGCGACCTCAGATC207850.21224799061350239No Hit
TAGCTTATCAGACTGATGTTG207310.21169656451327967No Hit
AAGCTGCCAGTTGAAGAACTGC196010.20015746278639696No Hit
TTCACAGTGGCTAAGTTCCG195000.19912609174709148No Hit
TTCAAGTAATTCAGGATAGGT191540.1955928800678867No Hit
AAAAGCTGGGTTGAGAGGGCGA184420.1883222248205057No Hit
TGAGGTAGTAGGTTGTATAG183850.1877401639369373No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC182330.18618800158075482No Hit
CAACGGAATCCCAAAAGCAGCTG179160.18295092614055852No Hit
CATTGCACTTGTCTCGGTCTG177980.1817459579956274No Hit
TGAGGTAGTAGTTTGTGCTGTC177950.18171532321228168No Hit
TGAGGTAGTAGGTTGTATAGC177030.18077585652301337No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA175110.17881523038888816No Hit
TGAGGTAGTAGGTTGTATAGTC174500.17819232312752545No Hit
TGTAAACATCCCCGACTGGAAG172090.17573132886542037No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC171390.17501651725402056No Hit
TGAGATGAAGCACTGTAGCTC170760.1743731868037607No Hit
AGCTCGGTCTGAGGCCCCTCAGT161580.1649989430999746No Hit
TACAGTATAGATGATGTACT161230.16464153729427466No Hit
TCAGTGCATCACAGAACTTTGT160830.1642330735163319No Hit
AAACCGTTACCATTACTG159380.16275239232128944No Hit
TGAGGTAGTAGATTGTATAGTC152630.15585956606850548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA150930.15412359501224882No Hit
AAACCGTTACCATTACTGAGA147690.1508150384109125No Hit
TGAGATGAAGCACTGTAGCT144860.14792515718196755No Hit
TTCAAGTAATCCAGGATAGGCA142310.1453212005975825No Hit
TGGAGTGTGACAATGGTGTTT141700.1446982933362198No Hit
TACAGTACTGTGATAACTGAAG139140.1420841251573862No Hit
CAACGGAATCCCAAAAGCAGCT136200.13908191638950698No Hit
TGAGGGGCAGAGAGCGAGACTT131810.13459902642658528No Hit
TAGCAGCACGTAAATATTGGCG131360.13413950467639968No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT126840.12952386398564658No Hit
TCCTGTACTGAGCTGCCCCGAA126130.1287988407797982No Hit
TGAGGTAGTAGATTGTATAGC124070.12669525232339304No Hit
TGAGGTAGTAGTTTGTACAGC122810.12540859142287336No Hit
CTAGACTGAAGCTCCTTGAGG120130.12267188411065692No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT119910.1224472290327884No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC118950.1214669159657258No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG117590.12007813912072046No Hit
TATTGCACTTGTCCCGGCCTG113610.11601392453019006No Hit
TGTAAACATCCCCGACTGGAAGC113370.11576884626342442No Hit
AAGCTGCCAGTTGAAGAACT111860.11422689550169053No Hit
TGAGGGGCAGAGAGCGAGACTTC109100.11140849543388553No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC108910.11121447513936272No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGT108790.11109193600597991No Hit
TGGCTCAGTTCAGCAGGAACAG108390.11068347222803715No Hit
GGCTGGTCCGATGGTAGT106470.10872284609391196No Hit
TGAGGTAGTAGGTTGTGTGG104360.10656819966526393No Hit
GTAGTCGTGGCCGAGTGGTTAAGG104280.10648650690967539No Hit
GGGTGCTGTAGGCTT103980.1061801590762183No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT103170.10535301992588425No Hit
AAACCGTTACCATTACTGAGG102800.1049751909312872No Hit
TGAGGTAGTAGGTTGTGTGGC101200.10334133581951618No Hit
AGAGGTAGTAGGTTGCATAGTT100850.10298393001381628No Hit
AAACCGTTACCATTACTGAGTC100420.10254483145252782No Hit
TGAGGTAGTAGATTGTATAG99010.10110499663527964No Hit
AGGAAACTCTGGTG98830.1009211879352054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)