FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19003680
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT194450610.232260278009312No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC15411698.109845040539517No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT6554583.4491109090449847No Hit
TCCTGTACTGAGCTGCCCCGAG3467331.8245571384068768No Hit
TCCTGTACTGAGCTGCCCCGA2821621.484775580308656No Hit
TCAGTGCACTACAGAACTTTGT2774251.4598488292793816No Hit
CGCGACCTCAGATCAGA2202801.1591439131789212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2188661.15170324905492No Hit
TATTGCACTTGTCCCGGCCTGT2128591.1200935818746685No Hit
TGAGGTAGTAGGTTGTATAGT1925731.0133458361748882No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1855550.9764161467673629No Hit
TAGCTTATCAGACTGATGTTGAC1808690.9517577648118679No Hit
TTCAAGTAATCCAGGATAGGCT1698590.8938216177077283No Hit
AAACCGTTACCATTACTGAGC1697390.8931901610635414No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1648760.8676003805578709No Hit
AAACCGTTACCATTACTGAG1602190.8430946006247211No Hit
TGAGGTAGTAGGTTGTATAGTT1597420.8405845604640786No Hit
TGAGGTAGTAGATTGTATAGT1320970.6951127360595422No Hit
TGAGGGGCAGAGAGCGAGACTTT1284240.6757849006087242No Hit
TGAGGTAGTAGATTGTATAGTT1245450.6553730645853856No Hit
CATTGCACTTGTCTCGGTCTGA1099270.578451121046029No Hit
AAGCTGCCAGTTGAAGAACTGT1094240.575804265279146No Hit
TGAGGGGCAGAGAGCGAGACT1058420.5569552844501696No Hit
TGAGGTAGTAGGTTGTGTGGT1047160.5510301162722168No Hit
TCGTACCGTGAGTAATAATGCG1023540.5386009446591398No Hit
TGAGGTAGTAGGTTGTGTGGTT963030.5067597433760198No Hit
TCCTGTACTGAGCTGCCCCG960060.5051968881816574No Hit
TCCTGTACTGAGCTGCCCCGAGA952930.5014449832874475No Hit
TAGCTTATCAGACTGATGTTGA949690.4997400503481431No Hit
TGAGGTAGTAGTTTGTGCTGTT920460.48435881892349275No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT852100.44838683875965074No Hit
AAAAGCTGGGTTGAGAGGGCGA823700.43344236484722964No Hit
TGAGGTAGTAGGTTGTATAG797530.41967134786525556No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT762120.4010381147230431No Hit
TGAGGTAGTAGTTTGTACAGT755150.39737040404805807No Hit
AAACCGTTACCATTACTG731830.38509909659602776No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT709760.37348555648169196No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC654770.34454905576183137No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC618790.32561588071363023No Hit
TGAGGTAGTAGGTTGTGTGG611430.32174294662928443No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG584380.3075088614415734No Hit
CTAGACTGAAGCTCCTTGAGG580560.30549872445757875No Hit
TCCTGTACTGAGCTGCCCCGAGT573480.3017731302568766No Hit
TAGCTTATCAGACTGATGTTG569930.29990507101782393No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA538740.2834924604076684No Hit
CATTGCACTTGTCTCGGTCTG528410.27805667112896026No Hit
TGGAGTGTGACAATGGTGTTT516570.27182629890631704No Hit
TGGCTCAGTTCAGCAGGAAC502180.2642540813147769No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT483850.2546085810748234No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA473160.24898335480285924No Hit
TGAGGTAGTAGATTGTATAG468500.2465311981679338No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC468280.24641543111649952No Hit
TGTAAACATCCCCGACTGGAAG448030.23575960024584713No Hit
CCCATAAAGTAGAAAGCACT442390.23279175401816912No Hit
TTCACAGTGGCTAAGTTCCG418650.22029943674067337No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA414340.2180314549603024No Hit
TATTGCACTTGTCCCGGCCTG412320.21696850294258796No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA372180.19584627819453915No Hit
TGTAAACATCCCCGACTGGAAGCT369110.19423080161316122No Hit
TGAGGTAGTAGTTTGTACAGTT365850.19251534439645374No Hit
TGGCTCAGTTCAGCAGGAACAG358730.18876870164094534No Hit
CGCGACCTCAGATC334620.176081685231492No Hit
AAACCGTTACCATTACTGAGTT327370.17226663467286335No Hit
TGAGAACTGAATTCCATGGGTT327240.17219822686974312No Hit
TGAGGGGCAGAGAGCGAGACTT320950.16888834162646393No Hit
TACAGTACTGTGATAACTGAAG316480.16653616562686807No Hit
TTCAAGTAATTCAGGATAGGT308040.16209492056275418No Hit
AAAAGCTGGGTTGAGAGGGCG298800.15723270440251574No Hit
TAGCAGCACGTAAATATTGGCG290670.1529545856381501No Hit
TGAGGTAGTAGTTTGTGCTGT289550.15236522610357572No Hit
AAATGGATTTTTGGAGCAGGGAGA280870.14779768971062449No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAAC275480.14496139695048538No Hit
TCAGTGCACTACAGAACTTTGC263010.1383995099896441No Hit
CGCGACCTCAGATCAGACGT260890.13728393658491408No Hit
TGTAAACATCCCCGACTGGAAGC253010.13313737128808736No Hit
CAACGGAATCCCAAAAGCAGCTG250520.13182709875139972No Hit
CTAGACTGAAGCTCCTTGAGGA235820.12409175486011131No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC232090.12212897712443065No Hit
AGCTCGGTCTGAGGCCCCTCAGT219960.1157460028794423No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT212140.11163101041482491No Hit
TGTAAACATCCTTGACTGGAAGCT209050.11000500955604389No Hit
TATTGCACTTGTCCCGGCCTGC208200.10955772776641155No Hit
TGAGGTAGTAGGTTGTAT203140.10689508558342385No Hit
TGAGATGAAGCACTGTAGCTC200990.10576372576258915No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA198560.10448502605811084No Hit
AAGCTGCCAGTTGAAGAACT194690.10244857838060839No Hit
TAGCAGCACGTAAATATTGGC192220.10114883012132386No Hit
TTCAAGTAATCCAGGATAGGCC191430.10073312116390089No Hit
AAAAGCTGGGTTGAGAGGGCGT191290.10065945122207909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)