FastQCFastQC Report
Wed 8 May 2024
EGAF00002167540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16289785
Total Bases377.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9094865.583167610867792No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5385313.305942957503736No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5193373.1881145147096785No Hit
TTCAAGTAATCCAGGATAGGCT1940451.1912066365516794No Hit
TCAGTGCACTACAGAACTTTGT1877561.1525996199458741No Hit
AAACCGTTACCATTACTGAGC1669161.024666685287743No Hit
CGCGACCTCAGATCAGA1451280.8909141526422847No Hit
TGAGGTAGTAGGTTGTATAGTT1413940.8679918120466291No Hit
TGAGGTAGTAGGTTGTATAGT1374210.8436022943212572No Hit
TATTGCACTTGTCCCGGCCTGT1325720.8138351733924052No Hit
TAGCTTATCAGACTGATGTTGAC1294630.7947495930732051No Hit
TCCTGTACTGAGCTGCCCCGAG1262390.7749580488631372No Hit
TCCTGTACTGAGCTGCCCCGA1206300.7405254274381153No Hit
TGAGGTAGTAGATTGTATAGTT1137750.6984438407259519No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1041560.6393945653671917No Hit
TGAGGGGCAGAGAGCGAGACTTT943180.5790008892075617No Hit
TGAGGTAGTAGATTGTATAGT921180.5654954930344385No Hit
TGGAGTGTGACAATGGTGTTT857910.5266552014038245No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT769370.4723021206234459No Hit
AAGCTGCCAGTTGAAGAACTGT765490.4699202598438224No Hit
TGAGGTAGTAGTTTGTGCTGTT757890.4652547593476526No Hit
TGAGGTAGTAGGTTGTGTGGT736780.45229571783789657No Hit
TGAGGTAGTAGGTTGTGTGGTT725050.445094886151045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC668980.4106745423589077No Hit
TCGTACCGTGAGTAATAATGCG618450.3796551028758206No Hit
TGAGGGGCAGAGAGCGAGACT602030.36957516627751685No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT553410.33972824073491453No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT541970.3327054347248905No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT538840.3307839851784416No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC532710.3270208906992941No Hit
TAGCTTATCAGACTGATGTTGA501560.30789847748144006No Hit
TGAGGTAGTAGTTTGTACAGT480670.2950744899334153No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT477950.2934047318611019No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA437240.26841361012438164No Hit
TTCAAGTAATCCAGGATAGGCC435430.2673024843483201No Hit
TGTAAACATCCCCGACTGGAAGCT411860.2528332939937513No Hit
TCCTGTACTGAGCTGCCCCGAGA389050.23883065368879944No Hit
TCCTGTACTGAGCTGCCCCGAGT387410.23782388779225752No Hit
TCAGTGCACTACAGAACTTTGC363380.22307231188134158No Hit
AAAAGCTGGGTTGAGAGGGCGA356100.21860325351132628No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT347060.2130537634474611No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT344590.21153747578620588No Hit
TCCTGTACTGAGCTGCCCCG327780.2012181253466513No Hit
CATTGCACTTGTCTCGGTCTGA320740.19689639857125188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT318090.19526961221403474No Hit
TATTGCACTTGTCCCGGCCTGC313050.19217564872710105No Hit
CTAGACTGAAGCTCCTTGAGG312900.19208356648046612No Hit
CCCATAAAGTAGAAAGCACT300970.18475995846476795No Hit
CGCGACCTCAGATCAGACGT296340.18191768645196973No Hit
AAACCGTTACCATTACTGAG294910.18103983570071672No Hit
TAGCTTATCAGACTGATGTTG292340.17946215987503825No Hit
TGAGGTAGTAGTTTGTACAGTT281240.17264807362405335No Hit
TGAGGTAGTAGGTTGTATAGTC278120.1707327628940468No Hit
TGAGAACTGAATTCCATGGGTT273530.1679150461470179No Hit
TGGCTCAGTTCAGCAGGAAC259900.15954783933612382No Hit
TGAGGTAGTAGGTTGTATAGC258030.15839988066140837No Hit
TAGCTTATCAGACTGATGTTGAT254520.15624515609015097No Hit
TTCAAGTAATTCAGGATAGGT224120.13758315410547162No Hit
TGAGGTAGTAGGTTGTATAG221360.13588884076738889No Hit
TGAGGTAGTAGGTTGTGTGG218160.1339244195058437No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC218050.13385689252497807No Hit
TGAGGTAGTAGATTGTATAGTC217510.13352539643709233No Hit
AGCTCGGTCTGAGGCCCCTCAGT213840.13127245080275768No Hit
TGAGGGGCAGAGAGCGAGACTT208170.1277917418799573No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG204140.12531779885369881No Hit
TTCACAGTGGCTAAGTTCCG190340.11684623216328516No Hit
AAACCGTTACCATTACTG177740.10911132344595094No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA177670.10906835173085465No Hit
TTCAAGTAATCCAGGATAGGCA177410.1089087425033541No Hit
TGAGGTAGTAGATTGTATAGC174020.10682768372940465No Hit
TAGCTTATCAGACTGATGTTGACT169880.10428621372228057No Hit
TCGTACCGTGAGTAATAATGCA163950.10064589557197963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)