FastQCFastQC Report
Thu 9 May 2024
EGAF00002167541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27203604
Total Bases613.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT295050010.845989376995783No Hit
TTCAAGTAATCCAGGATAGGCT7502002.7577228370182127No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6561822.412114218395474No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5539832.036432378592189No Hit
AAACCGTTACCATTACTGAGC4931561.8128333289956728No Hit
TAGCTTATCAGACTGATGTTGAC4544181.6704330793816877No Hit
TGAGGTAGTAGGTTGTATAGTT3856351.4175879048967188No Hit
TCAGTGCACTACAGAACTTTGT3843561.412886321974103No Hit
TGAGGTAGTAGGTTGTATAGT3686121.355011637428629No Hit
TGAGGTAGTAGATTGTATAGTT3506081.2888292301270081No Hit
TGAGGTAGTAGATTGTATAGT2625650.9651846130387723No Hit
TGAGGTAGTAGTTTGTGCTGTT2553080.9385080006310929No Hit
TATTGCACTTGTCCCGGCCTGT2485380.9136215921978573No Hit
CGCGACCTCAGATCAGA2367840.8704140818988543No Hit
TCGTACCGTGAGTAATAATGCG2339350.859941204849181No Hit
AAGCTGCCAGTTGAAGAACTGT2314940.8509681290758386No Hit
TGAGGTAGTAGGTTGTGTGGT2250300.8272065715998512No Hit
TGAGGGGCAGAGAGCGAGACTTT2234520.8214058696046304No Hit
TCCTGTACTGAGCTGCCCCGA2187060.8039596518167226No Hit
TCCTGTACTGAGCTGCCCCGAG2117130.7782534990584336No Hit
TGAGGTAGTAGGTTGTGTGGTT2019880.7425045593223604No Hit
TGGAGTGTGACAATGGTGTTT2007240.7378581161525509No Hit
TTCAAGTAATCCAGGATAGGCC1552100.5705494022042079No Hit
TGAGGGGCAGAGAGCGAGACT1506510.5537905933346184No Hit
TAGCTTATCAGACTGATGTTGA1485000.5458835527821976No Hit
TGAGGTAGTAGTTTGTACAGT1461460.5372302875751316No Hit
TAGCTTATCAGACTGATGTTG1073800.3947271104225749No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1030710.37888729743309013No Hit
TGTAAACATCCCCGACTGGAAGCT946100.34778480086682634No Hit
AAACCGTTACCATTACTGAG937760.344719030610797No Hit
CTAGACTGAAGCTCCTTGAGG821800.3020923257080202No Hit
CCCATAAAGTAGAAAGCACT810170.297817156873773No Hit
TTCAAGTAATTCAGGATAGGT793540.29170399627931654No Hit
TGAGGTAGTAGTTTGTACAGTT763550.28067972170158045No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT750720.27596343484488306No Hit
CGCGACCTCAGATCAGACGT749940.2756767081302904No Hit
AAAAGCTGGGTTGAGAGGGCGA743880.27344906211691655No Hit
TGAGAACTGAATTCCATGGGTT729160.26803801437486No Hit
TCCTGTACTGAGCTGCCCCGAGT723250.2658655081142925No Hit
TAGCTTATCAGACTGATGTTGAT700940.25766438888023807No Hit
TGGCTCAGTTCAGCAGGAAC697450.25638147063161193No Hit
TGAGGTAGTAGGTTGTGTGG674560.24796714435337316No Hit
TGAGGTAGTAGGTTGTATAGTC674260.24785686484776062No Hit
TCAGTGCACTACAGAACTTTGC670300.24640117537367473No Hit
TCCTGTACTGAGCTGCCCCGAGA667450.24535352007035538No Hit
TGAGGTAGTAGGTTGTATAG660510.24280238750718472No Hit
TGAGGTAGTAGGTTGTATAGC630920.2319251522702654No Hit
TCCTGTACTGAGCTGCCCCG620500.22809477744198894No Hit
TGAGGTAGTAGATTGTATAGTC606410.22291531666171882No Hit
CATTGCACTTGTCTCGGTCTGA598560.22002966959819No Hit
AAACCGTTACCATTACTG594330.21847472856905284No Hit
TTCAAGTAATCCAGGATAGGCA564410.2074761858759597No Hit
TTCACAGTGGCTAAGTTCCG560650.2060940160722822No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG524170.19268402818979427No Hit
TAGCTTATCAGACTGATGTTGACT516170.1897432413734592No Hit
TATTGCACTTGTCCCGGCCTGC515110.1893535871202948No Hit
TCGTACCGTGAGTAATAATGCA508960.1870928572552372No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT508950.18708918127171678No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT502070.18456010460966862No Hit
AGCTCGGTCTGAGGCCCCTCAGT500700.18405649486737125No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA491080.18052019872072833No Hit
TGAGGTAGTAGTTTGTGCTGT488850.17970045439567492No Hit
TGAGGTAGTAGTTTGTGCTGTC449080.1650810679349692No Hit
TGAGGTAGTAGGTTGTAT445930.16392313312603726No Hit
TGAGGTAGTAGATTGTATAGC440360.16187561030516398No Hit
TGAGGTAGTAGATTGTAT439660.16161829145873466No Hit
TGAGGTAGTAGGTTGTGTGGC418050.15367449107110956No Hit
AAGCTGCCAGTTGAAGAACTGC418000.15365611115350744No Hit
AAACCGTTACCATTACTGAGA417330.15340982025763938No Hit
CAACGGAATCCCAAAAGCAGCT413930.15215998586069698No Hit
TGAGGGGCAGAGAGCGAGACTT410640.15095058728247918No Hit
AAGCTGCCAGTTGAAGAACT410010.1507190003206928No Hit
TCGTACCGTGAGTAATAATGC406860.14956106551176088No Hit
TGAGGTAGTAGATTGTATAG391080.14376036351653995No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT387940.14260610469112842No Hit
TGTAAACATCCTTGACTGGAAGCT386020.14190031585520801No Hit
TGAGGTAGTAGGTTGTGTGGTC380110.13972780959464048No Hit
CTAGACTGAAGCTCCTTGAGGA378780.13923890378642478No Hit
AGAGGTAGTAGGTTGCATAGTT374140.13753324743295042No Hit
TGGCTCAGTTCAGCAGGAACAG366600.13476155585855462No Hit
TGAGGTAGTAGATTGTATAGTTT365770.13445644922635985No Hit
TTCAAGTAATTCAGGATAGGTT361890.13303016762043735No Hit
TGTAAACATCCCCGACTGGAAGC354400.13027685596364363No Hit
TACAGTATAGATGATGTACT351060.12904907746782374No Hit
CAACGGAATCCCAAAAGCAGCTG344520.12664498424546983No Hit
TAGCTTATCAGACTGATGTTGACA343550.1262884138439892No Hit
TAGTGGGTTATCAGAACT336160.12357186202239967No Hit
TGAGATGAAGCACTGTAGCTC327000.12020466111769602No Hit
TACCCTGTAGAACCGAATTTGT320670.1178777635492709No Hit
CGCGACCTCAGATC320110.11767190847212744No Hit
TATTGCACTTGTCCCGGCCTG316890.11648824177855258No Hit
AAAAGCTGGGTTGAGAGGGCGT300870.1105993161788416No Hit
TGGAGTGTGACAATGGTGTTC295510.1086289890118971No Hit
TCAGTGCATCACAGAACTTTGT295220.10852238548980495No Hit
TGAGGGGCAGAGAGCGAGACTTC294110.10811435131903846No Hit
TACAGTACTGTGATAACTGAAG289410.1063866390644416No Hit
TGAGGGGCAGAGAGCGAGACC287150.10555586678882695No Hit
TGTAAACATCCCCGACTGGAAG283300.1041406131334657No Hit
CATTGCACTTGTCTCGGTCTG281590.10351201995147408No Hit
AGAGGTAGTAGGTTGCATAGT280610.10315177356647304No Hit
AACCCGTAGATCCGATCTTGT280250.10301943815973795No Hit
TGAGGTAGTAGGTTGTGTGGTTT279430.10271800751106362No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC275930.10143141327891701No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT272630.10021833871717879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)