FastQCFastQC Report
Thu 9 May 2024
EGAF00002167542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10831449
Total Bases250 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT121587211.225386372589668No Hit
TCAGTGCACTACAGAACTTTGT3853923.558083502955145No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3352913.095532278275972No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2228672.0575917404956625No Hit
TGGAGTGTGACAATGGTGTTT1947531.7980327470498176No Hit
AAACCGTTACCATTACTGAGC1728231.595566761196955No Hit
CGCGACCTCAGATCAGA1692951.5629949418586562No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1622271.4977405146809075No Hit
TTCAAGTAATCCAGGATAGGCT1374351.2688514713036088No Hit
TGAGGTAGTAGGTTGTATAGT1252621.1564657692613425No Hit
TCCTGTACTGAGCTGCCCCGAG1203711.1113102226673457No Hit
TGAGGTAGTAGGTTGTATAGTT1074270.9918063594261488No Hit
TGAGGGGCAGAGAGCGAGACTTT1065790.9839773053448343No Hit
TATTGCACTTGTCCCGGCCTGT1027620.9487373296038231No Hit
TCCTGTACTGAGCTGCCCCGA993610.9173380219027021No Hit
AAGCTGCCAGTTGAAGAACTGT805510.7436770463490158No Hit
TGAGGTAGTAGATTGTATAGT768850.7098311592474839No Hit
TGAGGTAGTAGATTGTATAGTT729730.6737141078723631No Hit
TAGCTTATCAGACTGATGTTGAC681980.6296295167894895No Hit
TGAGGTAGTAGGTTGTGTGGT678800.6266936215089967No Hit
TGAGGGGCAGAGAGCGAGACT662320.6114786673509703No Hit
TGAGGTAGTAGGTTGTGTGGTT635870.5870590352223419No Hit
TAGCTTATCAGACTGATGTTGA587340.5422543188819889No Hit
TGAGGTAGTAGTTTGTGCTGTT581280.5366594995738797No Hit
TCAGTGCACTACAGAACTTTGC574140.5300675837554144No Hit
AAACCGTTACCATTACTGAG492630.454814494348817No Hit
TGGCTCAGTTCAGCAGGAAC481920.4449266206211191No Hit
TGAGGTAGTAGTTTGTACAGT436320.40282699018386187No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC433110.39986339777808116No Hit
CCCATAAAGTAGAAAGCACT421460.38910768079136965No Hit
TCGTACCGTGAGTAATAATGCG405860.37470517564178163No Hit
TCCTGTACTGAGCTGCCCCGAGA400000.3692950038355902No Hit
TTCACAGTGGCTAAGTTCCG381930.35261210203731747No Hit
CGCGACCTCAGATCAGACGT369180.34084082379005803No Hit
TCCTGTACTGAGCTGCCCCGAGT363680.3357630174873187No Hit
AAATGGATTTTTGGAGCAGGGAGA359130.3315622868186888No Hit
AAAAGCTGGGTTGAGAGGGCGA349490.32266227722625107No Hit
TAGCTTATCAGACTGATGTTG348190.32146206846378544No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC331250.30582242505134816No Hit
TCCTGTACTGAGCTGCCCCG324150.29926743873326644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT315750.29151224365271905No Hit
TGAGGTAGTAGGTTGTATAG298510.2755956289874051No Hit
TGAGGTAGTAGGTTGTGTGG284430.26259644485239236No Hit
CATTGCACTTGTCTCGGTCTGA281390.2597898028232419No Hit
TGAGGGGCAGAGAGCGAGACTT281350.2597528733228583No Hit
AAACCGTTACCATTACTG265450.24507339692039354No Hit
TTCAAGTAATCCAGGATAGGCC255040.23546249444557232No Hit
TGTAAACATCCCCGACTGGAAGCT253030.23360678705129848No Hit
TGGAGTGTGACAATGGTGTTC249080.22995999888842206No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT224960.20769151015713594No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT214580.1981083048076024No Hit
TTCAAGTAATTCAGGATAGGT214480.19801598105664348No Hit
TGAGAACTGAATTCCATGGGTT211690.1954401484048902No Hit
AAATGGATTTTTGGAGCAGGGAG211010.19481234689836974No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA203130.1875372353228086No Hit
TGAGGTAGTAGTTTGTACAGTT199880.18453671341664443No Hit
TATTGCACTTGTCCCGGCCTGC197860.1826717736472747No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT193500.17864645810546675No Hit
TGTAAACATCCCCGACTGGAAG189910.17533203544604234No Hit
TGTAAACATCCTTGACTGGAAGCT189350.17481502244067254No Hit
AAATGGATTTTTGGAGCAGGGA187760.17334707480042605No Hit
TGAGGTAGTAGGTTGTATAGC180980.16708752448541278No Hit
TGGAGTGTGACAATGGTGTTTG178210.16453015658385134No Hit
AAATGGATTTTTGGAGCAGGGAGAT173040.15975701865927633No Hit
TGAGGTAGTAGTTTGTGCTGT172510.15926770277919416No Hit
TGTAAACATCCCCGACTGGAAGC171430.1582706062688381No Hit
TGGCTCAGTTCAGCAGGAACAG170150.15708886225656418No Hit
CGTGTATTTGACAAGCTGAGTTG165380.1526850193358248No Hit
CGCGACCTCAGATC163040.15052464356338657No Hit
CTAGACTGAAGCTCCTTGAGG162460.14998916580782498No Hit
AAGCTGCCAGTTGAAGAACT162000.14956447655341404No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC160590.1482627116648936No Hit
TGAGGTAGTAGGTTGTATAGTC157920.14579766751429102No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT156490.1444774378755788No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC155770.14381270686867473No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT155220.1433049262384008No Hit
TGAGATGAAGCACTGTAGCTC154110.14228013260275701No Hit
TGAGGTAGTAGATTGTATAG153680.14188314047363376No Hit
TGAGATGAAGCACTGTAGCT151260.13964890570042846No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC148420.13702691117319576No Hit
AACCCGTAGATCCGATCTTGT141890.13099817023557975No Hit
TATTGCACTTGTCCCGGCCTG137720.1271482698205937No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA135670.12525563292593633No Hit
CAACGGAATCCCAAAAGCAGCT135360.12496942929796373No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA135160.12478478179604593No Hit
AAATGGATTTTTGGAGCAGGG134240.12393540328722408No Hit
AGCTCGGTCTGAGGCCCCTCAGT132120.12197813976689545No Hit
TGAGGTAGTAGGTTGTAT130720.12068560725347088No Hit
AAACCGTTACCATTACTGAGTT129220.11930075098908742No Hit
AAGCTGCCAGTTGAAGAACTGC128590.11871911135804639No Hit
ATTTTTGGAGCAGGGAGA127310.11753736734577248No Hit
AAAAGCTGGGTTGAGAGGGCGT125500.11586630745341643No Hit
AAATGGATTTTTGGAAATAGGAGA125080.11547854769938908No Hit
TTTTTGGAGCAGGGAGA124200.11466609869095078No Hit
CGCGACCTCAGATCAGAC123100.11365053743040289No Hit
AACATTCAACGCTGTCGGTGA122220.11283808842196459No Hit
ACCAATATTACTGTGCTGCTT121910.11255188479399202No Hit
AAAAGCTGGGTTGAGAGGGCG119960.11075157165029352No Hit
CATTGCACTTGTCTCGGTCTG117950.10889586425601967No Hit
AGAGGTAGTAGGTTGCATAGT117760.10872044912919776No Hit
TGAGGTAGTAGGTTGTGTGGC114330.10555374447130758No Hit
AAACCGTTACCATTACTGAGA113670.10494440771497886No Hit
TGAGGGGCAGAGAGCGAGACTTC112080.1034764600747324No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA110800.1022947160624585No Hit
TACAGTACTGTGATAACTGAAG110020.10157459080497909No Hit
GGCTGGTCCGATGGTAGTGGGTTATC109330.1009375569233627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)