FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10490497
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT120178711.455958664303513No Hit
TCAGTGCACTACAGAACTTTGT4063093.8731148772074384No Hit
TTCAAGTAATCCAGGATAGGCT2065031.9684768033392506No Hit
AAACCGTTACCATTACTGAGC1837911.751976097986587No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1582811.5088036343749966No Hit
CGCGACCTCAGATCAGA1554511.4818268381374113No Hit
TGGAGTGTGACAATGGTGTTT1283591.2235740594559057No Hit
TATTGCACTTGTCCCGGCCTGT1201981.1457798424612295No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1195301.1394121746567394No Hit
TGAGGTAGTAGGTTGTATAGTT1171381.116610585752038No Hit
TGAGGGGCAGAGAGCGAGACTTT1071351.0212576201108488No Hit
TGAGGTAGTAGGTTGTATAGT1042320.9935849559844495No Hit
AAGCTGCCAGTTGAAGAACTGT1004330.9573712284556203No Hit
TCCTGTACTGAGCTGCCCCGAG995360.9488206326163574No Hit
TAGCTTATCAGACTGATGTTGAC971100.9256949408593321No Hit
TGAGGTAGTAGATTGTATAGTT803750.766169610457922No Hit
TCCTGTACTGAGCTGCCCCGA721150.687431682216772No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT696990.6644013148280773No Hit
TCAGTGCACTACAGAACTTTGC660000.629140830982555No Hit
TGAGGTAGTAGATTGTATAGT648610.6182833854296894No Hit
TAGCTTATCAGACTGATGTTGA608320.5798771974292543No Hit
TGAGGGGCAGAGAGCGAGACT587420.55995440444814No Hit
AAACCGTTACCATTACTGAG584010.5567038434880636No Hit
TGAGGTAGTAGTTTGTGCTGTT560870.5346457846563418No Hit
CCCATAAAGTAGAAAGCACT533070.5081456102604099No Hit
TCGTACCGTGAGTAATAATGCG505510.4818742143484718No Hit
TGAGGTAGTAGGTTGTGTGGTT500890.4774702285315939No Hit
TGAGGTAGTAGGTTGTGTGGT497590.47432452437668104No Hit
TGGCTCAGTTCAGCAGGAAC458730.43728147484337493No Hit
CGCGACCTCAGATCAGACGT408280.38919033102054174No Hit
TTCAAGTAATCCAGGATAGGCC405480.38652124870728244No Hit
TGAGGTAGTAGTTTGTACAGT379370.3616320561361392No Hit
TGTAAACATCCCCGACTGGAAGCT372170.35476870161632956No Hit
TTCACAGTGGCTAAGTTCCG358960.34217635255984535No Hit
CGCGACCTCAGATC352460.3359802686183505No Hit
TGAGAACTGAATTCCATGGGTT340780.3248463823973259No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC329940.3145132208702791No Hit
TAGCTTATCAGACTGATGTTG320120.3051523679002053No Hit
TCCTGTACTGAGCTGCCCCGAGA297700.2837806445204646No Hit
TCCTGTACTGAGCTGCCCCGAGT295960.28212200051151054No Hit
CATTGCACTTGTCTCGGTCTGA288830.27532537304953236No Hit
AAAAGCTGGGTTGAGAGGGCGA274100.26128409359442173No Hit
TGAGGGGCAGAGAGCGAGACTT269170.25658460223571866No Hit
TCCTGTACTGAGCTGCCCCG262990.25069355627288203No Hit
AAACCGTTACCATTACTG262590.25031225879955926No Hit
TATTGCACTTGTCCCGGCCTGC244490.23305854813170435No Hit
CTAGACTGAAGCTCCTTGAGG233640.22271585416782447No Hit
TGGCTCAGTTCAGCAGGAACAG233570.222649127109993No Hit
TGAGGTAGTAGGTTGTGTGG230700.21991331773890216No Hit
TGAGGTAGTAGGTTGTATAG218000.20780712296090453No Hit
TGTAAACATCCTTGACTGGAAGCT213960.20395601848034467No Hit
AAACCGTTACCATTACTGAGTT208920.19915167031647785No Hit
TGAGGTAGTAGTTTGTACAGTT208070.19834141318566698No Hit
AAATGGATTTTTGGAGCAGGGAGA207850.19813169957533946No Hit
TGAGATGAAGCACTGTAGCTC205730.19611082296672883No Hit
TGTAAACATCCCCGACTGGAAGC204940.1953577604569164No Hit
TGTAAACATCCCCGACTGGAAG201660.19223112117566976No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC200740.1913541369870274No Hit
AGCTCGGTCTGAGGCCCCTCAGT195820.18666417806515745No Hit
TGAGATGAAGCACTGTAGCT194830.1857204668186836No Hit
TGGAGTGTGACAATGGTGTTC189360.18050622387099485No Hit
TGAGGTAGTAGGTTGTATAGTC188740.17991521278734457No Hit
GGCTGGTCCGATGGTAGT188160.17936233145102656No Hit
TTCAAGTAATTCAGGATAGGT187090.17834236070988818No Hit
TAGCTTATCAGACTGATGTTGAT170640.16266150211948965No Hit
AAGCTGCCAGTTGAAGAACTGC169180.1612697663418616No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC165380.15764744034529535No Hit
AAGCTGCCAGTTGAAGAACT161030.15350083032291034No Hit
TGAGGTAGTAGGTTGTATAGC160770.15325298696525055No Hit
TGAGGTAGTAGTTTGTGCTGT159520.15206143236111694No Hit
AACCCGTAGATCCGATCTTGT154110.1469043840344266No Hit
AAACCGTTACCATTACTGAGA149010.1420428412495614No Hit
AACATTCAACGCTGTCGGTGA145300.13850630718449278No Hit
CAACGGAATCCCAAAAGCAGCT144660.13789623122717637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT142520.1358562897448996No Hit
TCAGTGCATCACAGAACTTTGT142140.13549405714524296No Hit
TTCAAGTAATCCAGGATAGGCA140640.13406419162028263No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT139050.13254853416382464No Hit
CGTGTATTTGACAAGCTGAGTTG137550.1311186686388643No Hit
AAATGGATTTTTGGAGCAGGGAGAT135220.12889761085675921No Hit
TGAGGGGCAGAGAGCGAGACTTC134630.12833519708360816No Hit
TGAGGTAGTAGGTTGTAT134370.12808735372594834No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC133340.12710551273214224No Hit
GGCTGGTCCGATGGTAGTGGGT131270.12513229830769695No Hit
AGAGGTAGTAGGTTGCATAGTT129800.12373103009323581No Hit
TTTGGAGCAGGGAGA128720.12270152691526437No Hit
TGAGGTAGTAGATTGTATAGTC128110.12212004826844716No Hit
TAGCTTATCAGACTGATGTTGACT126000.1201087040966696No Hit
GGCTGGTCCGATGGTAGTGGGTT124460.11864070882437695No Hit
TATTGCACTTGTCCCGGCCTG122680.11694393506809068No Hit
CAACGGAATCCCAAAAGCAGCTG119420.11383636066051017No Hit
CGCGACCTCAGATCAGAC118690.11314049277169613No Hit
CATTGCACTTGTCTCGGTCTG115380.10998525617995028No Hit
TGAGGTAGTAGATTGTATAG114220.1088794935073143No Hit
GGCTGGTCCGATGGTAGTG112550.10728757655619175No Hit
CTAGACTGAAGCTCCTTGAGGA109610.10448504012726947No Hit
TGAGGTAGTAGATTGTAT109450.10433252113794036No Hit
AAAAGCTGGGTTGAGAGGGCGT108900.10380823711212156No Hit
GGCTGGTCCGATGGTAGTGGGTTATC108260.10319816115480515No Hit
TGGCTCAGTTCAGCAGGAACA106890.1018922173086747No Hit
AAATGGATTTTTGGAGCAGGGAG105820.10087224656753632No Hit
AAATGGATTTTTGGAGCAGGGA105790.10084364925703711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)