FastQCFastQC Report
Wed 8 May 2024
EGAF00002167544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12056192
Total Bases274.9 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11374619.434662288059116No Hit
TCAGTGCACTACAGAACTTTGT3866373.2069578852095257No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2773522.3004942190701674No Hit
TGGAGTGTGACAATGGTGTTT2389621.9820686332798945No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2319071.923550985253055No Hit
TTCAAGTAATCCAGGATAGGCT1973721.6371006699296098No Hit
TGAGGTAGTAGGTTGTATAGT1880781.5600116521037488No Hit
AAACCGTTACCATTACTGAGC1698211.408579093630891No Hit
TGAGGTAGTAGGTTGTATAGTT1695111.406007800804765No Hit
TATTGCACTTGTCCCGGCCTGT1410441.1698884689294928No Hit
TCCTGTACTGAGCTGCCCCGAG1278891.0607744136788797No Hit
TGAGGGGCAGAGAGCGAGACTTT1250661.0373590599751563No Hit
TGAGGTAGTAGGTTGTGTGGT1144200.9490558876301904No Hit
TGAGGTAGTAGATTGTATAGT1084940.8999027221862426No Hit
TGAGGTAGTAGATTGTATAGTT1073050.8900405700241004No Hit
CGCGACCTCAGATCAGA1064760.8831644353374598No Hit
TGAGGTAGTAGGTTGTGTGGTT1056330.876172177748994No Hit
TAGCTTATCAGACTGATGTTGAC1009980.8377272027519138No Hit
TGAGGTAGTAGTTTGTGCTGTT955470.7925139214770301No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC934970.7755102108526474No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT915180.7590954092303772No Hit
AAGCTGCCAGTTGAAGAACTGT914360.7584152608054019No Hit
TCCTGTACTGAGCTGCCCCGA895680.7429211479047447No Hit
TGAGGGGCAGAGAGCGAGACT718030.5955694799817388No Hit
TAGCTTATCAGACTGATGTTGA616640.5114716155814374No Hit
TCAGTGCACTACAGAACTTTGC607420.5038240930469587No Hit
TCGTACCGTGAGTAATAATGCG605010.5018251202369703No Hit
TGAGGTAGTAGTTTGTACAGT559770.46430083396150296No Hit
AAACCGTTACCATTACTGAG487810.4046136624234252No Hit
TGGCTCAGTTCAGCAGGAAC446710.3705232962447844No Hit
TCCTGTACTGAGCTGCCCCGAGA422610.35053356814490017No Hit
CCCATAAAGTAGAAAGCACT417760.34651073904596075No Hit
TCCTGTACTGAGCTGCCCCGAGT414440.3437569673741095No Hit
CATTGCACTTGTCTCGGTCTGA397250.32949873392859036No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC383270.31790303273206No Hit
TTCAAGTAATCCAGGATAGGCC375640.3115743345825946No Hit
AAATGGATTTTTGGAGCAGGGAGA374380.3105292284661691No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC368930.3060087297879795No Hit
TGAGGTAGTAGGTTGTGTGG368210.3054115262928792No Hit
TGTAAACATCCCCGACTGGAAGCT365750.30337108101795324No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT357860.29682672605081273No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC349600.2899754748431346No Hit
TTCACAGTGGCTAAGTTCCG338570.28082664907791777No Hit
TCCTGTACTGAGCTGCCCCG327650.27176906273556356No Hit
TGGAGTGTGACAATGGTGTTC324470.26913141396553736No Hit
TAGCTTATCAGACTGATGTTG324440.2691065304865749No Hit
CGCGACCTCAGATCAGACGT318010.2637731714956099No Hit
TGAGAACTGAATTCCATGGGTT315320.26154195288197135No Hit
TGAGGTAGTAGGTTGTATAG313370.25992452674940814No Hit
CTAGACTGAAGCTCCTTGAGG303450.2516963897058043No Hit
TGAGGTAGTAGTTTGTACAGTT288420.239229766745586No Hit
AAAAGCTGGGTTGAGAGGGCGA285080.2364594060877597No Hit
TGAGGTAGTAGGTTGTATAGC283450.2351074037307966No Hit
TATTGCACTTGTCCCGGCCTGC278470.23097674622301967No Hit
TGGCTCAGTTCAGCAGGAACAG274150.22739352525241804No Hit
TGAGGGGCAGAGAGCGAGACTT266750.22125560044166515No Hit
TGAGGTAGTAGGTTGTATAGTC259910.21558216723821252No Hit
AAACCGTTACCATTACTG242820.20140687872256843No Hit
TTCAAGTAATTCAGGATAGGT242320.20099215407319326No Hit
TGGAGTGTGACAATGGTGTTTG222880.1848676597054858No Hit
CGCGACCTCAGATC219600.18214706600558453No Hit
TGTAAACATCCCCGACTGGAAG214980.17831501024535773No Hit
AAACCGTTACCATTACTGAGTT210710.1747732617396936No Hit
TGTAAACATCCTTGACTGGAAGCT203530.1688178157746658No Hit
TGAGGTAGTAGTTTGTGCTGT200410.16622993396256464No Hit
TGTAAACATCCCCGACTGGAAGC199520.16549172408667678No Hit
TGAGGTAGTAGGTTGTGTGGC198790.164886226098589No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT196620.16308632112030066No Hit
TGAGATGAAGCACTGTAGCTC195970.1625471790761129No Hit
AGCTCGGTCTGAGGCCCCTCAGT193550.160539911773137No Hit
TGAGGTAGTAGGTTGTAT191610.15893078013356127No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT181490.15053675323020735No Hit
TGAGGTAGTAGGTTGTGTGGTC180100.14938381870494433No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT174340.1446061907441421No Hit
TGAGATGAAGCACTGTAGCT171160.14196854197411587No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT170370.14131327702810306No Hit
TGAGGTAGTAGATTGTATAGC160250.13291925012474917No Hit
AGAGGTAGTAGGTTGCATAGTT159460.1322639851787364No Hit
TGAGGTAGTAGATTGTATAGTC159390.13220592372782386No Hit
CGTGTATTTGACAAGCTGAGTTG158990.13187414400832367No Hit
AACCCGTAGATCCGATCTTGT157220.1304060187495355No Hit
TGAGGTAGTAGATTGTATAG156900.13014059497393538No Hit
CATTGCACTTGTCTCGGTCTG154730.12834068999564704No Hit
TGAGGTAGTAGTTTGTGCTGTC152750.1266983803841213No Hit
TGAGGTAGTAGATTGTAT151290.1254873844079457No Hit
CAACGGAATCCCAAAAGCAGCT150630.12493994787077048No Hit
AAGCTGCCAGTTGAAGAACTGC149940.1243676278546327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA147150.12205346431111913No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC145910.12102494718066867No Hit
TGAGGGGCAGAGAGCGAGACTTC145760.1209005297858561No Hit
AGAGGTAGTAGGTTGCATAGT145650.12080929036299357No Hit
TAGCTTATCAGACTGATGTTGAT144620.11995495758528067No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG143560.11907574132860525No Hit
TGAGGTAGTAGGTTGTGTGGTTT142130.1178896288313922No Hit
TCAGTGCATCACAGAACTTTGT141970.11775691694359212No Hit
TAGCTTATCAGACTGATGTTGACT139210.11546763687904109No Hit
AAGCTGCCAGTTGAAGAACT138880.11519391861045346No Hit
TATTGCACTTGTCCCGGCCTG136310.11306223391266497No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT135800.11263921477030227No Hit
GGCTGGTCCGATGGTAGTGGGTT135180.11212495620507702No Hit
AAATGGATTTTTGGAGCAGGGAGAT134760.11177658749960187No Hit
CTAGACTGAAGCTCCTTGAGGA133600.11081442631305143No Hit
GGCTGGTCCGATGGTAGTGGGTTATC131850.10936289004023823No Hit
CAACGGAATCCCAAAAGCAGCTG131820.10933800656127574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT130840.10852514624850036No Hit
TACAGTATAGATGATGTACT129360.10729756128634979No Hit
AAATGGATTTTTGGAAATAGGAGA127460.10572160761872405No Hit
TGAGGTAGTAGTTTGTGCT126220.10469309048827358No Hit
AAACCGTTACCATTACTGAGA125460.10406270902122328No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT125410.10402123655628577No Hit
GGCTGGTCCGATGGTAGT124730.1034572110331355No Hit
TTCAAGTAATCCAGGATAGGCA123860.10273559014322269No Hit
GGCTGGTCCGATGGTAGTGGGT121870.10108498603870941No Hit
AACATTCAACGCTGTCGGTGA121100.10044631007867161No Hit
AAAAGCTGGGTTGAGAGGGCGT121030.1003882486277591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)