FastQCFastQC Report
Wed 8 May 2024
EGAF00002167545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11809668
Total Bases273.3 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8608307.289197291575005No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5795224.90718282681613No Hit
TCCTGTACTGAGCTGCCCCGAG2125631.799906652752643No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1846571.5636087314224243No Hit
TCAGTGCACTACAGAACTTTGT1598031.353154042941766No Hit
TCCTGTACTGAGCTGCCCCGA1563441.3238644812030278No Hit
TATTGCACTTGTCCCGGCCTGT1369401.15955842281087No Hit
CGCGACCTCAGATCAGA1271591.0767364501694714No Hit
TGAGGTAGTAGGTTGTATAGT1125740.9532359419418056No Hit
TAGCTTATCAGACTGATGTTGAC1036030.8772727565245695No Hit
TTCAAGTAATCCAGGATAGGCT1020650.8642495284372091No Hit
AAACCGTTACCATTACTGAG1010940.8560274514067626No Hit
AAACCGTTACCATTACTGAGC980680.8304043771594596No Hit
TGAGGTAGTAGGTTGTATAGTT806190.6826525521293232No Hit
CATTGCACTTGTCTCGGTCTGA801180.6784102652166005No Hit
TGAGGTAGTAGATTGTATAGT767180.649620294152215No Hit
TAGCTTATCAGACTGATGTTGA714620.6051143859420942No Hit
TGAGGTAGTAGATTGTATAGTT663830.5621072497550312No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC660290.5591097057089158No Hit
TGAGGTAGTAGTTTGTGCTGTT641920.5435546536955992No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT638840.5409466210227079No Hit
TGAGGGGCAGAGAGCGAGACTTT635270.5379236740609474No Hit
TCCTGTACTGAGCTGCCCCGAGA604230.511640124006873No Hit
AAGCTGCCAGTTGAAGAACTGT594270.5032063560127177No Hit
CGCGACCTCAGATC571120.4836037727732905No Hit
TGAGGTAGTAGGTTGTGTGGT568110.48105501357023756No Hit
TGAGGTAGTAGGTTGTGTGGTT550780.4663805959659493No Hit
TCCTGTACTGAGCTGCCCCG543930.46058026356033044No Hit
TGAGGTAGTAGTTTGTACAGT522930.44279822260879814No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG511260.4329164884228753No Hit
TGAGGTAGTAGGTTGTATAG490250.41512597983279464No Hit
TGAGGGGCAGAGAGCGAGACT419180.3549464726696805No Hit
TCCTGTACTGAGCTGCCCCGAGT410860.34790139739745435No Hit
TAGCTTATCAGACTGATGTTG401490.3399672200776516No Hit
AAAAGCTGGGTTGAGAGGGCGA385270.32623271035223006No Hit
TCGTACCGTGAGTAATAATGCG384550.32562304037674894No Hit
TGGCTCAGTTCAGCAGGAAC366930.31070306125455854No Hit
CATTGCACTTGTCTCGGTCTG364800.3088994542437603No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC359970.3048095848249079No Hit
AAACCGTTACCATTACTG357280.3025317900554021No Hit
CCCATAAAGTAGAAAGCACT327390.2772220184343878No Hit
TGTAAACATCCCCGACTGGAAG320870.2717011181008645No Hit
TACAGTACTGTGATAACTGAAG305040.2582968462788285No Hit
TGAGGTAGTAGATTGTATAG296590.25114169170547385No Hit
CGCGACCTCAGATCAGAC269760.22842301748025431No Hit
TGAGGTAGTAGGTTGTGTGG268240.22713593642090532No Hit
TGGAGTGTGACAATGGTGTTT258490.21887998883626533No Hit
TGTAAACATCCCCGACTGGAAGCT256990.21760984305401304No Hit
TTCACAGTGGCTAAGTTCCG252030.2134098943340321No Hit
TATTGCACTTGTCCCGGCCTG248080.21006517710743436No Hit
AAACCGTTACCATTACTGAGTT243080.20583135783326No Hit
CGCGACCTCAGATCAGACGT230300.19500971576847037No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC224000.1896751034830107No Hit
CTAGACTGAAGCTCCTTGAGG218930.1853820107389979No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC213430.18072480953740613No Hit
TGAAAAGAACTTTGAAGAGAG211740.1792937786227352No Hit
TGAGGTAGTAGTTTGTACAGTT197420.16716812022149988No Hit
TCAGTGCACTACAGAACTTTGC196320.16623667998118152No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT194210.16445000824747993No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA187830.15904765485363348No Hit
TGAGATGAAGCACTGTAGCTC185250.15686300410815954No Hit
TGTAAACATCCCCGACTGGAAGC184550.15627026940977512No Hit
TTCAAGTAATTCAGGATAGGT183530.15540657027784355No Hit
TGTAAACATCCTTGACTGGAAGCT176590.14953002912528954No Hit
TAGCAGCACGTAAATATTGGCG176540.1494876909325478No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT170350.14424622267111997No Hit
TGAGGTAGTAGTTTGTGCTGT167850.14212931303403278No Hit
TATTGCACTTGTCCCGGCCTGC167610.1419260897088724No Hit
TGAGAACTGAATTCCATGGGTT167280.14164665763677692No Hit
TGAGGGGCAGAGAGCGAGACTT156210.1322729817637549No Hit
CAACGGAATCCCAAAAGCAGCTG152450.1290891496695758No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA151560.12833552983877278No Hit
TTCAAGTAATCCAGGATAGGCC146850.12434727208250054No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT146610.12414404875734017No Hit
AAAAGCTGGGTTGAGAGGGCG143300.12134126039783676No Hit
TACAGTACTGTGATAACTGA136410.1155070574380245No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA135670.1148804521854467No Hit
TGAGGTAGTAGGTTGTATAGC134680.11404215596916019No Hit
TAGCAGCACGTAAATATTGGC131140.11104461192304475No Hit
CGCGACCTCAGATCAGACG131020.11094300026046457No Hit
TGAGGTAGTAGGTTGTAT127960.10835190286466985No Hit
TGGCTCAGTTCAGCAGGAACAG124140.10511726493920068No Hit
TACAGTATAGATGATGTACT124080.10506645910791058No Hit
GGCTGGTCCGATGGTAGTGGGTTATC122450.10368623402452974No Hit
TGTAAACATCCCCGACTGGAA121960.10327131973566066No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC120010.10162013021873266No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG120000.10161166258018431No Hit
GGAATACCGGGTGCTGTAGGCTT118680.10049393429180228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)