FastQCFastQC Report
Wed 8 May 2024
EGAF00002167546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12263441
Total Bases277.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT133800610.910526662133408No Hit
TCAGTGCACTACAGAACTTTGT3224252.6291560419298303No Hit
AAACCGTTACCATTACTGAGC2928222.387763760595415No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2520422.0552306648680414No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1826451.489345445540122No Hit
TGAGGTAGTAGGTTGTATAGT1727711.4088297077467897No Hit
TTCAAGTAATCCAGGATAGGCT1709861.3942742497803022No Hit
CGCGACCTCAGATCAGA1707651.392472145460642No Hit
TATTGCACTTGTCCCGGCCTGT1678261.3685066043046157No Hit
TGAGGGGCAGAGAGCGAGACTTT1341901.0942279577159462No Hit
TGAGGTAGTAGGTTGTATAGTT1324371.0799334379314909No Hit
TGAGGTAGTAGGTTGTGTGGT1181470.9634082310177055No Hit
TCCTGTACTGAGCTGCCCCGAG1177110.9598529482875158No Hit
TCCTGTACTGAGCTGCCCCGA1140780.9302283103086647No Hit
TGAGGTAGTAGGTTGTGTGGTT1120750.9138952109770822No Hit
TGAGGTAGTAGATTGTATAGT1058510.8631427345718057No Hit
AAGCTGCCAGTTGAAGAACTGT986360.8043093288417175No Hit
TGAGGTAGTAGATTGTATAGTT967790.7891667599656572No Hit
TGAGGTAGTAGTTTGTGCTGTT936990.7640514599450513No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT865310.7056013071698228No Hit
TGAGGGGCAGAGAGCGAGACT817030.6662322589557043No Hit
TCAGTGCACTACAGAACTTTGC746140.608426297317368No Hit
TGGAGTGTGACAATGGTGTTT696370.5678422556931615No Hit
TAGCTTATCAGACTGATGTTGAC652990.5324688233914119No Hit
TGAGGTAGTAGTTTGTACAGT628940.5128576881480491No Hit
TCCTGTACTGAGCTGCCCCGAGT538740.4393057380877031No Hit
CCCATAAAGTAGAAAGCACT503550.410610692382342No Hit
TAGCTTATCAGACTGATGTTGA494490.40322288010355334No Hit
CATTGCACTTGTCTCGGTCTGA482930.3937964882776376No Hit
AAACCGTTACCATTACTGAG472340.38516106531600713No Hit
TATTGCACTTGTCCCGGCCTGC456930.37259526098751566No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC452800.3692275275756617No Hit
TTCAAGTAATCCAGGATAGGCC448570.36577825098192257No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT427460.34856448528598133No Hit
TCCTGTACTGAGCTGCCCCGAGA396170.3230496236741385No Hit
TGAGGTAGTAGGTTGTATAGC377070.30747487593408734No Hit
CGCGACCTCAGATCAGACGT359900.2934739116044184No Hit
TGGCTCAGTTCAGCAGGAAC354980.2894619870556722No Hit
TCCTGTACTGAGCTGCCCCG338510.2760318249991988No Hit
TGTAAACATCCCCGACTGGAAGCT331520.27033195658543147No Hit
TGAGGTAGTAGGTTGTATAGTC324800.2648522547627538No Hit
AAACCGTTACCATTACTGAGA308910.2518950431612139No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC299940.24458061974612183No Hit
AAAAGCTGGGTTGAGAGGGCGA295040.2405850038337527No Hit
TGAGGTAGTAGGTTGTGTGGTC293640.2394433992873615No Hit
TAGCTTATCAGACTGATGTTG289120.2357576474661557No Hit
TGAGGGGCAGAGAGCGAGACTT288310.23509714769288653No Hit
TGAGGTAGTAGGTTGTGTGGC287060.23407785791932298No Hit
AAACCGTTACCATTACTGAGTT274790.22407250950202312No Hit
TGAGGTAGTAGGTTGTGTGG263650.21498859904002474No Hit
TGAGAACTGAATTCCATGGGTT260100.21209381608310426No Hit
TGAGGTAGTAGGTTGTATAG257580.2100389278996001No Hit
AAACCGTTACCATTACTGAGG256250.2089544035805285No Hit
TCGTACCGTGAGTAATAATGCG255590.20841621858008696No Hit
TGAGGGGCAGAGAGCGAGACTTC255260.20814712607986618No Hit
TGAGGTAGTAGTTTGTACAGTT249290.2032789981213266No Hit
TTCAAGTAATTCAGGATAGGT245620.20028636334614405No Hit
TGTAAACATCCTTGACTGGAAGCT242870.19804392584430422No Hit
TGAGGTAGTAGTTTGTGCTGT239030.194912667659917No Hit
AAGCTGCCAGTTGAAGAACTGC236140.19255606970343805No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG234380.1911209097022606No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT232670.18972652129202563No Hit
TGAGGTAGTAGATTGTATAGTC231520.18878877470034713No Hit
TGAGGTAGTAGATTGTATAGC228050.18595922628893471No Hit
TGAGGTAGTAGTTTGTGCTGTC227580.18557597333407483No Hit
CGCGACCTCAGATC222210.1811971044668458No Hit
TTCACAGTGGCTAAGTTCCG219120.1786774201465967No Hit
TGAGGTAGTAGGTTGTAT212670.17341788491500876No Hit
AAACCGTTACCATTACTG210470.17162393491353692No Hit
TAGCTTATCAGACTGATGTTGAT205870.16787294854682305No Hit
TGAGGGGCAGAGAGCGAGACC204420.1666905724094893No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT203590.1660137639998431No Hit
TCCTGTACTGAGCTGCCCCGAA201030.16392625854358495No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT192940.15732941512908163No Hit
TTCAAGTAATCCAGGATAGGCA184040.15007207194130912No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC181280.1478214801212808No Hit
AGCTCGGTCTGAGGCCCCTCAGT177240.14452713557312338No Hit
CATTGCACTTGTCTCGGTCTG176370.14381770989072318No Hit
TGTAAACATCCCCGACTGGAAG168280.13722086647621984No Hit
AAGCTGCCAGTTGAAGAACT167060.1362260396572218No Hit
TGAGGTAGTAGATTGTAT166010.13536983624742843No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT165150.1346685648832167No Hit
CAACGGAATCCCAAAAGCAGCT163980.13371450965516124No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC163470.1332986394275473No Hit
TACAGTATAGATGATGTACT162780.13273599147254023No Hit
TGAGATGAAGCACTGTAGCT156470.1275906166955914No Hit
AAAAGCTGGGTTGAGAGGGCGT155750.1270035057860188No Hit
ACCAATATTACTGTGCTGCTT155270.1266120985129704No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT153470.12514432123903885No Hit
CTAGACTGAAGCTCCTTGAGG151290.12336667987394402No Hit
TGGAGTGTGACAATGGTGTTC150800.12296711828270711No Hit
TGAGATGAAGCACTGTAGCTC146170.1191916689614277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT143670.1171530894143006No Hit
TCAGTGCATCACAGAACTTTGT141910.11571792941312313No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA139030.1133694857748327No Hit
TGAGGTAGTAGTTTGTACAGC138450.1128965353198992No Hit
CGCGACCTCAGATCAGAC138270.11274975759250605No Hit
AGAGGTAGTAGGTTGCATAGT134640.10978974009007748No Hit
TACAGTACTGTGATAACTGAAG132720.10822411099788388No Hit
AACCCGTAGATCCGATCTTGT132540.10807733327049071No Hit
TATTGCACTTGTCCCGGCCTG132340.10791424690672057No Hit
TGAGGTAGTAGATTGTATAG130870.10671556213300983No Hit
TCCTGTACTGAGCTGCCCCGAGC124400.10143971826504486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)