FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11524075
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6741605.850013992446248No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC4133323.5866826621659436No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4125393.579801415731848No Hit
TCAGTGCACTACAGAACTTTGT2467332.141022164468732No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1548151.343405002136831No Hit
TCCTGTACTGAGCTGCCCCGAG1356921.177465436488395No Hit
CGCGACCTCAGATCAGA1266431.0989428652625048No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1202121.04313795250378No Hit
TTCAAGTAATCCAGGATAGGCT1147450.9956981362929347No Hit
TATTGCACTTGTCCCGGCCTGT1058240.9182862832808707No Hit
TAGCTTATCAGACTGATGTTGAC1049180.9104244809236316No Hit
TGAGGTAGTAGGTTGTATAGT929250.8063553907797372No Hit
TCCTGTACTGAGCTGCCCCGA906460.7865794000820022No Hit
CATTGCACTTGTCTCGGTCTGA896120.7776068795109369No Hit
AAACCGTTACCATTACTGAGC852380.7396515555478422No Hit
TGAGGTAGTAGGTTGTATAGTT722270.6267487846096108No Hit
TAGCTTATCAGACTGATGTTGA667570.5792829359406286No Hit
TGAGGTAGTAGATTGTATAGT665290.5773044691222506No Hit
AAACCGTTACCATTACTGAG648630.562847777370418No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT634130.5502654226044174No Hit
TGAGGTAGTAGATTGTATAGTT628500.5453799979607907No Hit
CGCGACCTCAGATC561640.48736232626045906No Hit
TGAGGTAGTAGTTTGTGCTGTT547470.4750663285339604No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC520220.45142017905992454No Hit
TGAGGTAGTAGGTTGTGTGGT485700.4214654972307973No Hit
AAGCTGCCAGTTGAAGAACTGT480560.4170052694034011No Hit
TGAGGTAGTAGTTTGTACAGT471310.4089785948112972No Hit
TGAGGTAGTAGGTTGTGTGGTT465670.4040844926816252No Hit
TGAGGGGCAGAGAGCGAGACTTT453130.39320292518054595No Hit
TGGCTCAGTTCAGCAGGAAC448900.38953234858329194No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT436840.3790673004123975No Hit
TCGTACCGTGAGTAATAATGCG431520.3744508778361821No Hit
TCCTGTACTGAGCTGCCCCGAGA417150.3619813303887731No Hit
TGAGGTAGTAGGTTGTATAG414340.35954295680998255No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC392500.34059132728657177No Hit
TAGCTTATCAGACTGATGTTG364010.3158691695428917No Hit
CCCATAAAGTAGAAAGCACT359560.31200768825263636No Hit
TTCACAGTGGCTAAGTTCCG343270.2978720634844879No Hit
TCAGTGCACTACAGAACTTTGC340550.2955117872801071No Hit
CATTGCACTTGTCTCGGTCTG314870.27322800311521744No Hit
CGCGACCTCAGATCAGACGT313030.2716313456828422No Hit
TGAGATGAAGCACTGTAGCTC312430.27111069652011116No Hit
TCCTGTACTGAGCTGCCCCG310380.26933181188078No Hit
TGAAAAGAACTTTGAAGAGAG289860.2515256105153776No Hit
AAAAGCTGGGTTGAGAGGGCGA280980.24382000290695782No Hit
TGTAAACATCCCCGACTGGAAG269330.23371073166392967No Hit
TGAGGGGCAGAGAGCGAGACT261370.2268034527716975No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG259170.2248944058416836No Hit
TCCTGTACTGAGCTGCCCCGAGT248430.21557478582879755No Hit
TGAGGTAGTAGATTGTATAG243130.21097571822467315No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG239600.20791256565060537No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC237500.20609029358104664No Hit
AAACCGTTACCATTACTGAGTT235620.2044589262044893No Hit
AAACCGTTACCATTACTG233600.2027060740232947No Hit
TGGAGTGTGACAATGGTGTTT229690.19931317697949724No Hit
TACAGTACTGTGATAACTGAAG227780.19765577714480337No Hit
TGAGGTAGTAGTTTGTACAGTT224050.19441907484982524No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC206480.17917273186785057No Hit
TGAGGTAGTAGGTTGTGTGG200220.17374062560335646No Hit
CTAGACTGAAGCTCCTTGAGG195840.16993988671541968No Hit
TTCAAGTAATTCAGGATAGGT187380.16259873352091164No Hit
GCATTGGTGGTTCAGTGGTAGAATT186390.1617396624024054No Hit
CAACGGAATCCCAAAAGCAGCTG183580.1593012888236149No Hit
TTCAAGTAATCCAGGATAGGCC176630.15327043602198007No Hit
TGTAAACATCCCCGACTGGAAGCT173890.1508928048455082No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT170550.1479945245063053No Hit
TAGCAGCACGTAAATATTGGCG166390.1443846903113699No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC165080.14324793963940707No Hit
GTTGAAAAGAACTTTGAAGAGAG164620.14284877528131326No Hit
TGGCTCAGTTCAGCAGGAACAG162350.1408789859489807No Hit
TGTAAACATCCTTGACTGGAAGCT155300.1347613582868907No Hit
TGAGATGAAGCACTGTAGCT149630.12984122369908213No Hit
CGCGACCTCAGATCAGACG149160.1294333818549428No Hit
CGCGACCTCAGATCAGAC148110.12852224582016344No Hit
TATTGCACTTGTCCCGGCCTG147340.12785407939465857No Hit
TGAGGTAGTAGTTTGTGCTGT147100.12764581972956615No Hit
AGGAAACTCTGGTG146300.12695162084592473No Hit
TGAGAACTGAATTCCATGGGTT146130.12680410358315095No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA144450.12534628592750394No Hit
TATTGCACTTGTCCCGGCCTGC142680.1238103708974473No Hit
TGTAAACATCCCCGACTGGAAGC140250.12170174178838648No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA138440.12013111681414777No Hit
AAATGGATTTTTGGAGCAGGGAGA138140.11987079223278224No Hit
TGTCAGTTTGTCAAATACCCCAA137100.11896833368404838No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT132030.1145688482589709No Hit
AAGTTGAAAAGAACTTTGAAGAGAG127700.1108114968012617No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA126690.10993507071066441No Hit
TGAGGTAGTAGGTTGTATAGC123340.10702811288541597No Hit
TAGCAGCACGTAAATATTGGC123310.1070020804272794No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA121960.10583061981113452No Hit
TCAGTGCATCACAGAACTTTGT120470.10453767439035236No Hit
AGTTGAAAAGAACTTTGAAGAGAG119550.10373934567416473No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA118100.10248111019756467No Hit
CGTGTATTTGACAAGCTGAGTTG116100.10074561298846112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)