FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762684
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8997855.367785970313585No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6986144.1676738641616105No Hit
TCAGTGCACTACAGAACTTTGT4455512.6579931948845426No Hit
TCCTGTACTGAGCTGCCCCGAG3872442.310155104039425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3461822.065194332840731No Hit
CGCGACCTCAGATCAGA3041161.8142440673581866No Hit
TGAGGTAGTAGGTTGTATAGT2497171.4897196654187361No Hit
TGAGGTAGTAGGTTGTATAGTT1921211.14612313875272No Hit
TCCTGTACTGAGCTGCCCCGA1908241.1383857143641196No Hit
TTCAAGTAATCCAGGATAGGCT1820711.0861685395966423No Hit
TGAGGTAGTAGATTGTATAGT1600910.955043953581658No Hit
TAGCTTATCAGACTGATGTTGAC1600530.9548172595748985No Hit
TGAGGTAGTAGGTTGTGTGGT1334560.7961493517386595No Hit
TGAGGTAGTAGATTGTATAGTT1319730.78730231984329No Hit
TATTGCACTTGTCCCGGCCTGT1308130.7803821870053745No Hit
TGAGGTAGTAGGTTGTGTGGTT1216380.7256475156365174No Hit
TCGTACCGTGAGTAATAATGCG1195590.713244967214081No Hit
TGAGGGGCAGAGAGCGAGACTTT1125580.6714795792845585No Hit
TGAGGTAGTAGTTTGTGCTGTT1109810.6620717780040476No Hit
AAGCTGCCAGTTGAAGAACTGT1077720.6429280656964004No Hit
TCCTGTACTGAGCTGCCCCGAGA1023700.610701722946039No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1001620.5975296080269723No Hit
CATTGCACTTGTCTCGGTCTGA992900.592327577135022No Hit
TAGCTTATCAGACTGATGTTGA955800.5701950833172063No Hit
TGAGGTAGTAGGTTGTATAG944720.5635851633306456No Hit
AAACCGTTACCATTACTGAG926280.5525845383710628No Hit
AAACCGTTACCATTACTGAGC881840.5260732708437384No Hit
TGAGGTAGTAGTTTGTACAGT819720.48901476637034974No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC816930.487350355110196No Hit
TGAGGGGCAGAGAGCGAGACT815130.48627654139396764No Hit
AAAAGCTGGGTTGAGAGGGCGA726850.4336119442447284No Hit
TGGCTCAGTTCAGCAGGAAC697560.41613860882899184No Hit
TCCTGTACTGAGCTGCCCCG662870.3954438322645705No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC584970.3489715608789141No Hit
CCCATAAAGTAGAAAGCACT572370.34145486486531634No Hit
TAGCTTATCAGACTGATGTTG567020.3382632518754156No Hit
TGAGGTAGTAGATTGTATAG536040.31978172469277594No Hit
TCCTGTACTGAGCTGCCCCGAGT533590.31832014491235416No Hit
TGAGGTAGTAGGTTGTGTGG518810.30950294117576876No Hit
TTCACAGTGGCTAAGTTCCG493220.294236889510057No Hit
CGCGACCTCAGATCAGACGT486750.29037712576339203No Hit
TGGCTCAGTTCAGCAGGAACAG431070.25716048814139786No Hit
TGAGGTAGTAGTTTGTACAGTT430930.25707696929680235No Hit
CTAGACTGAAGCTCCTTGAGG424480.25322913681365106No Hit
TGTAAACATCCCCGACTGGAAG419260.25011507703658914No Hit
CATTGCACTTGTCTCGGTCTG400080.23867299532700134No Hit
TGTAAACATCCCCGACTGGAAGCT380000.2266940067592994No Hit
TCAGTGCACTACAGAACTTTGC367560.21927276085381078No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT346120.20648244636718083No Hit
AAACCGTTACCATTACTG338130.20171590659347868No Hit
AAATGGATTTTTGGAGCAGGGAGA325560.19421710747515136No Hit
CGCGACCTCAGATC324380.19351316292784618No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT319670.19070335037038222No Hit
TGTAAACATCCCCGACTGGAAGC312340.18633054229263046No Hit
TGGAGTGTGACAATGGTGTTT308110.18380708005949406No Hit
TACAGTACTGTGATAACTGAAG298610.17813972989051155No Hit
TTCAAGTAATTCAGGATAGGT294340.17559240513034788No Hit
AAAAGCTGGGTTGAGAGGGCG286150.17070655272150928No Hit
TGAGGGGCAGAGAGCGAGACTT283790.16929866362689888No Hit
TATTGCACTTGTCCCGGCCTG270970.16165072371465095No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC264800.15796992892069076No Hit
CAACGGAATCCCAAAAGCAGCT262420.1565501085625667No Hit
TGTAAACATCCTTGACTGGAAGCT253520.15124069629899364No Hit
TAGCAGCACGTAAATATTGGC251990.15032795464019963No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT247860.14786414872463144No Hit
CGTGTATTTGACAAGCTGAGTTG244520.14587162771785234No Hit
AGAGGTAGTAGGTTGCATAGT244220.14569265876514764No Hit
CGCGACCTCAGATCAGAC235270.14035341834279044No Hit
TAGCAGCACGTAAATATTGGCG232680.13880831971777313No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC231720.13823561906911805No Hit
TGAGATGAAGCACTGTAGCTC230160.13730498051505355No Hit
CAACGGAATCCCAAAAGCAGCTG227730.1358553319981454No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG225070.1342684739508303No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA224170.1337315670927162No Hit
TGAGAACTGAATTCCATGGGTT219230.1307845450048453No Hit
TGGAGAGAAAGGCAGTTCCTGA218360.13026553504200164No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT211520.12618504292033425No Hit
AAGCTGCCAGTTGAAGAACT210980.12586289880546575No Hit
TGAGGTAGTAGTTTGTGCTGT208350.12429393765342113No Hit
TGAGGTAGTAGGTTGTAT206610.12325591772773382No Hit
AGAGGTAGTAGGTTGCATAGTT205860.12280849534597205No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA200040.11933649766350067No Hit
AACATTCAACGCTGTCGGTGA199930.11927087571417559No Hit
AAACCGTTACCATTACTGAGTT196570.11726642344388286No Hit
TGAGGTAGTAGGTTGTATAGC194570.11607329709251812No Hit
TCGTACCGTGAGTAATAATGC193690.11554832149791763No Hit
AAAAGCTGGGTTGAGAGGGCGAA191970.11452223283574398No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA190690.11375863197087054No Hit
TATTGCACTTGTCCCGGCCTGTA190450.11361545680870677No Hit
TGGCTCAGTTCAGCAGGAACA185120.11043577508231975No Hit
AGCTCGGTCTGAGGCCCCTCAGT180450.10764982505188309No Hit
GGCTGGTCCGATGGTAGTGGGTTATC180440.10764385942012628No Hit
AAATGGATTTTTGGAGCAGGGAG180010.10738733725458285No Hit
TGTAAACATCCCCGACTGGAA177720.10602120758227024No Hit
TTCAAGTAATCCAGGATAGGCC176800.10547236946064246No Hit
TACAGTATAGATGATGTACT176200.10511443155523303No Hit
AGCAGCATTGTACAGGGCTATG172750.10305628859912887No Hit
CTAGACTGAAGCTCCTTGAGGA172170.1027102819572331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)