FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505861
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT116790111.116661452117063No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4405044.19293573368237No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2916982.7765263599052No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2329442.217276623020236No Hit
TATTGCACTTGTCCCGGCCTGT1917101.8247909428841673No Hit
TCCTGTACTGAGCTGCCCCGAG1818581.7310147164520833No Hit
TCCTGTACTGAGCTGCCCCGA1430951.3620492408951537No Hit
AAACCGTTACCATTACTGAGC1313561.2503116117755604No Hit
TTCAAGTAATCCAGGATAGGCT1179131.1223544648077868No Hit
TCAGTGCACTACAGAACTTTGT1093411.0407619137546176No Hit
TGAGGTAGTAGGTTGTATAGT1041120.9909896961324731No Hit
TGAGGTAGTAGGTTGTGTGGT963360.9169738682055664No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC853020.811946779040766No Hit
CGCGACCTCAGATCAGA852530.8114803727176668No Hit
TGAGGTAGTAGGTTGTATAGTT740850.7051778050366362No Hit
TAGCTTATCAGACTGATGTTGAC674340.6418702855482288No Hit
TGGAGTGTGACAATGGTGTTT673460.6410326578659283No Hit
TGAGGTAGTAGATTGTATAGT672180.6398142903280368No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT647110.6159514198788657No Hit
TGAGGGGCAGAGAGCGAGACTTT610450.5810566121139429No Hit
TGAGGTAGTAGATTGTATAGTT558770.5318650227715749No Hit
TGAGGTAGTAGGTTGTGTGGTT540290.5142748414432667No Hit
TGAGGGGCAGAGAGCGAGACT540110.5141035085082507No Hit
TCCTGTACTGAGCTGCCCCGAGA536360.5105340723620844No Hit
AAACCGTTACCATTACTGAG535730.5099344070895284No Hit
TCCTGTACTGAGCTGCCCCGAGT525290.4999970968586011No Hit
TGAGGTAGTAGTTTGTGCTGTT524980.499702023470518No Hit
TAGCTTATCAGACTGATGTTGA497090.4731549370394297No Hit
TCCTGTACTGAGCTGCCCCG468160.44561792698380454No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC448670.42706637752012905No Hit
AAGCTGCCAGTTGAAGAACTGT395760.3767040131218184No Hit
TGAGGTAGTAGTTTGTACAGT394970.375952051907026No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC346900.3301966397613675No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT345350.32872127282095204No Hit
TCGTACCGTGAGTAATAATGCG339160.32282932355568006No Hit
TGTAAACATCCCCGACTGGAAGCT334290.3181938158138586No Hit
CGCGACCTCAGATCAGACGT306170.29142780396580537No Hit
TATTGCACTTGTCCCGGCCTGC302420.28785836781963897No Hit
CATTGCACTTGTCTCGGTCTGA301370.2868589256987124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC286980.2731618093938231No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG277180.26383368293184156No Hit
AAACCGTTACCATTACTG272830.2596931370022885No Hit
TAGCTTATCAGACTGATGTTG261230.24865168119014713No Hit
CCCATAAAGTAGAAAGCACT259680.24717631424973166No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC246340.234478640065769No Hit
TGAGGTAGTAGGTTGTGTGG238530.2270446943853531No Hit
CGGGGCGCGGCCGGA235780.2244271078781644No Hit
AGCGCACGTGTTAGGAC235340.2240082940370142No Hit
GCATTGGTGGTTCAGTGGTAGAATT230440.21934423080602344No Hit
TATTGCACTTGTCCCGGCCTG226500.21559394322845124No Hit
TGAGGTAGTAGGTTGTATAG218180.2076745542321567No Hit
CGCGACCTCAGATC215400.20502841223579865No Hit
AAACCGTTACCATTACTGAGTT208360.1983273907773956No Hit
TCTAACACGTGCGCGAGTCGGGGGCTC205960.1960429516438491No Hit
TTCAAGTAATTCAGGATAGGT205570.19567173028464777No Hit
CTAACACGTGCGCGAGTCGGGGGCTC202120.1923878490301747No Hit
AAAAGCTGGGTTGAGAGGGCGA202110.19237833053378492No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT196470.18700989856995062No Hit
TGTAAACATCCCCGACTGGAAG191550.1823267983461803No Hit
TGAGGTAGTAGTTTGTGCTGT176970.1684488306098853No Hit
TTCAAGTAATCCAGGATAGGCC173060.16472709852148243No Hit
TGAGAACTGAATTCCATGGGTT170320.16211903051068352No Hit
AGGGCGTGAAACCGTTAAGAGG170100.16190962359010844No Hit
TGAGGGGCAGAGAGCGAGACTT169280.1611291068861467No Hit
CTAGACTGAAGCTCCTTGAGG167920.15983459137713701No Hit
TGTAAACATCCCCGACTGGAAGC167110.15906359316956506No Hit
GTTCTATTTTGTTGGTTTTCGGAAC152210.1448810335487972No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC145780.1387606403701705No Hit
CATTGCACTTGTCTCGGTCTG143290.13639053476911603No Hit
AAGCTGCCAGTTGAAGAACT141510.1346962424117357No Hit
GGTGGGCGTAGCTC139240.13253554373125628No Hit
TGGCTCAGTTCAGCAGGAAC139070.13237372929263008No Hit
TGAGGTAGTAGTTTGTACAGTT138230.13157417559588883No Hit
ACCAATATTACTGTGCTGCTT138100.1314504351428217No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAC135320.12880429314646366No Hit
TGAGGTAGTAGGTTGTGTGGC133660.12722422274576067No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT133180.12676733491905137No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT131820.12547281941004168No Hit
TGAGGTAGTAGATTGTATAG131770.1254252269280928No Hit
TCAGTGCACTACAGAACTTTGC130140.12387371201655914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT126780.12067549722959403No Hit
TGAGGTAGTAGGTTGTAT126150.12007583195703807No Hit
GTCTGAGCGTCGCT124990.11897168637582393No Hit
TACAGTACTGTGATAACTGAAG119110.11337481049863501No Hit
AACCCGTAGATCCGATCTTGT117570.11190896205460932No Hit
TGAGGTAGTAGGTTGTATAGC115650.11008141074777213No Hit
CGCGACCTCAGATCAGAC113960.10847278485789981No Hit
TTCACAGTGGCTAAGTTCCG112220.10681656648607858No Hit
AGCTCGGTCTGAGGCCCCTCAGT110750.10541734751678136No Hit
CAACGGAATCCCAAAAGCAGCTG109890.10459875682726051No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT109260.10399909155470458No Hit
TGAGGTAGTAGATTGTAT109190.10393246207997611No Hit
CAACGGAATCCCAAAAGCAGCT108780.10354220372799526No Hit
CGCGACCTCAGATCAGACGTGG108210.10299964943377797No Hit
GGCTGGTCCGATGGTAGTGGGT107130.10197165182368204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)