FastQCFastQC Report
Wed 8 May 2024
EGAF00002167552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12283686
Total Bases306.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11093639.031189823640883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC9199757.489405053173778No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC9093567.402957060283046No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3440332.8007309857969345No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC2482492.0209650425776107No Hit
TCAGTGCACTACAGAACTTTGT2060301.677265276888387No Hit
TCCTGTACTGAGCTGCCCCGAG1547621.259898698159494No Hit
TATTGCACTTGTCCCGGCCTGT1532111.247272195007264No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1414251.151323796456536No Hit
TTCAAGTAATCCAGGATAGGCT1155520.9406948370383287No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC1094260.89082381298252No Hit
TGAGGTAGTAGGTTGTATAGT1072360.8729952882221184No Hit
TCCTGTACTGAGCTGCCCCGA1037460.8445836209098799No Hit
AAACCGTTACCATTACTGAGC898200.7312137415430515No Hit
AAACCGTTACCATTACTGAG896850.7301147228934377No Hit
TGAGGTAGTAGGTTGTATAGTT891070.7254092948973134No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT839110.683109288205511No Hit
CATTGCACTTGTCTCGGTCTGA824900.671541099308465No Hit
TAGCTTATCAGACTGATGTTGAC779670.6347199041069594No Hit
AAGCTGCCAGTTGAAGAACTGT752900.612926771329062No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT718930.5852722057532243No Hit
TAGCTTATCAGACTGATGTTGA708600.5768626778639571No Hit
TGAGGTAGTAGATTGTATAGT691090.5626079989345217No Hit
TGAGGTAGTAGATTGTATAGTT641490.5222292396598219No Hit
CGCGACCTCAGATCAGA566690.46133546559233113No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG559960.45585665410203424No Hit
TGAGGGGCAGAGAGCGAGACTTT510780.4158198117405476No Hit
TGAGGTAGTAGTTTGTGCTGTT509010.4143788761777206No Hit
TCGTACCGTGAGTAATAATGCG501180.4080045680099605No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC435480.3545189937287554No Hit
TCCTGTACTGAGCTGCCCCGAGA434750.3539247095700753No Hit
TGAGGTAGTAGTTTGTACAGT428530.3488610829029658No Hit
TGAGGTAGTAGGTTGTGTGGT423400.3446848120344333No Hit
TGAGGTAGTAGGTTGTGTGGTT422230.34373232920476804No Hit
AAACCGTTACCATTACTGAGTT406210.3306906412293508No Hit
CCCATAAAGTAGAAAGCACT405700.33027545640616346No Hit
TGAGGTAGTAGGTTGTATAG380370.309654610187854No Hit
TCCTGTACTGAGCTGCCCCG367910.2995110750958629No Hit
TTCACAGTGGCTAAGTTCCG366040.29798873074417564No Hit
TAGCTTATCAGACTGATGTTG361200.294048545363338No Hit
TGGCTCAGTTCAGCAGGAAC349220.28429577245787624No Hit
TGAGGGGCAGAGAGCGAGACT344230.28023347389374814No Hit
AAAAGCTGGGTTGAGAGGGCGA336740.2741359556081131No Hit
AAACCGTTACCATTACTG335130.27282527410746255No Hit
TGTAAACATCCCCGACTGGAAG316540.25769138025833616No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT312180.25414195706402787No Hit
CATTGCACTTGTCTCGGTCTG308530.2511705362706276No Hit
TCCTGTACTGAGCTGCCCCGAGT302950.24662792585222384No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC302670.24639998124341506No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC279420.22747243783339954No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA268750.21878612006200746No Hit
TGGCTCAGTTCAGCAGGAACAG264780.21555419114425425No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG263620.21460984919347498No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT261490.212875841990751No Hit
TGGAGTGTGACAATGGTGTTT249560.20316377347971937No Hit
TGTAAACATCCCCGACTGGAAGCT248550.2023415447122305No Hit
CTAGACTGAAGCTCCTTGAGG245840.2001353665341169No Hit
TGAGAACTGAATTCCATGGGTT240100.1954625020535367No Hit
CGCGACCTCAGATC239320.1948275135004265No Hit
TAGCAGCACGTAAATATTGGCG238160.19388317154964724No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT235610.19180724743371005No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA230950.1880135978728209No Hit
TGAGGTAGTAGTTTGTACAGTT220250.17930285746477076No Hit
TGAGGTAGTAGATTGTATAG212730.17318091654247755No Hit
TTCAAGTAATTCAGGATAGGT211980.17257035062602544No Hit
TATTGCACTTGTCCCGGCCTG209130.17025020014350742No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC206730.16829638921086063No Hit
TGTAAACATCCTTGACTGGAAGCT204120.16617161982160727No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT202200.16460857107548987No Hit
CGCGACCTCAGATCAGACGT200630.16333045309038344No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA200480.16320833990709302No Hit
TGAGGTAGTAGGTTGTGTGG192330.15657352361498006No Hit
TGTAAACATCCCCGACTGGAAGC187590.1527147470230027No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA187140.15234840747313144No Hit
CAACGGAATCCCAAAAGCAGCTG185530.15103772597248089No Hit
GCATTGGTGGTTCAGTGGTAGAATT177470.14447617759034218No Hit
TCAGTGCACTACAGAACTTTGC174540.14209090007673592No Hit
TGAGGTAGTAGTTTGTGCTGT169430.1379309109659755No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCG167350.13623760815768166No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG166140.13525256181247225No Hit
TGAGATGAAGCACTGTAGCTC165040.13435706513500917No Hit
TACAGTACTGTGATAACTGAAG153720.12514159023602522No Hit
TATTGCACTTGTCCCGGCCTGC152850.12443333377294079No Hit
TGAGGGGCAGAGAGCGAGACTT148290.12072109300091194No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT143580.11688673904559266No Hit
TCAGTGCATCACAGAACTTTGT136250.11091947482213402No Hit
TGAGATGAAGCACTGTAGCT132470.10784222260321535No Hit
TAGCAGCACGTAAATATTGGC131330.10691416241020814No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA126830.10325076691149546No Hit
GGCTGGTCCGATGGTAGTGGGTTATC126650.10310423109154696No Hit
CGTGTATTTGACAAGCTGAGTTG126250.10277859593610583No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC124320.10120740631110238No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT123310.10038517754361352No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT123130.10023864172366502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)