FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14560383
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT161445211.087977562128689No Hit
TCAGTGCACTACAGAACTTTGT3177862.1825387422844575No Hit
TATTGCACTTGTCCCGGCCTGT2777631.9076627311245864No Hit
AAACCGTTACCATTACTGAGC2270101.5590936035130394No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2181141.4979963095750983No Hit
TTCAAGTAATCCAGGATAGGCT1786831.2271861255298024No Hit
TCCTGTACTGAGCTGCCCCGAG1590681.0924712626034632No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1328260.9122424870279854No Hit
TCCTGTACTGAGCTGCCCCGA1280560.879482359770344No Hit
TGAGGTAGTAGGTTGTATAGTT1067020.73282412969494No Hit
CGCGACCTCAGATCAGA1047960.7197338146942975No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT995440.6836633349548566No Hit
TGAGGTAGTAGGTTGTATAGT965640.6631968403578394No Hit
CGCGACCTCAGATC906090.6222981909198405No Hit
CATTGCACTTGTCTCGGTCTGA896360.6156156743953781No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT895600.6150937101036422No Hit
AAGCTGCCAGTTGAAGAACTGT880230.6045376690984021No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC831260.570905312037465No Hit
TGAGGTAGTAGATTGTATAGTT805560.5532546774353394No Hit
CGCGACCTCAGATCAGACGT707140.48566030165552654No Hit
TGAGGGGCAGAGAGCGAGACTTT689780.4737375383600829No Hit
TAGCTTATCAGACTGATGTTGAC676380.4645344837426323No Hit
CCCATAAAGTAGAAAGCACT665990.4573986824385045No Hit
TGAGGTAGTAGATTGTATAGT648730.4455445986551315No Hit
TGAGGTAGTAGGTTGTGTGGTT634780.435963806721293No Hit
TGAGGTAGTAGGTTGTGTGGT616010.423072662305655No Hit
AAACCGTTACCATTACTGAG602820.41401383466355246No Hit
TGAGGGGCAGAGAGCGAGACT583970.40106774663825806No Hit
TAGCTTATCAGACTGATGTTGA578520.39732471323041435No Hit
TGAGGTAGTAGTTTGTGCTGTT562720.386473350323271No Hit
TGAGGTAGTAGTTTGTACAGT551450.3787331693129226No Hit
TATTGCACTTGTCCCGGCCTGC522390.3587749031052274No Hit
TCCTGTACTGAGCTGCCCCGAGT493050.3386243342637347No Hit
TCAGTGCACTACAGAACTTTGC472670.32462744970376123No Hit
TCCTGTACTGAGCTGCCCCGAGA468120.32150253190455225No Hit
TGGAGTGTGACAATGGTGTTT466220.3201976211752122No Hit
TCGTACCGTGAGTAATAATGCG439420.30179151194031095No Hit
TCCTGTACTGAGCTGCCCCG407860.2801162579308525No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC406320.27905859344496636No Hit
AAACCGTTACCATTACTGAGTT401480.27573450506075287No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC365730.2511815794955394No Hit
TGAGGTAGTAGTTTGTACAGTT350290.24057746283184994No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT350120.24046070766133007No Hit
TGGCTCAGTTCAGCAGGAAC325560.22359301949680857No Hit
TTCAAGTAATCCAGGATAGGCC322490.22148455847624338No Hit
TGAGGTAGTAGGTTGTATAG318750.21891594472480566No Hit
TGTAAACATCCCCGACTGGAAGCT316800.21757669423943035No Hit
AAACCGTTACCATTACTG305570.20986398503391016No Hit
TTCACAGTGGCTAAGTTCCG304710.20927334123010366No Hit
GCATTGGTGGTTCAGTGGTAGAATT302710.2078997509886931No Hit
TGAGGTAGTAGTTTGTGCTGT298870.2052624577251848No Hit
TGAGAACTGAATTCCATGGGTT295990.20328448777755365No Hit
CATTGCACTTGTCTCGGTCTG293560.20161557563423985No Hit
TAGCTTATCAGACTGATGTTG289880.19908816959004444No Hit
AAAAGCTGGGTTGAGAGGGCGA281930.19362814838043751No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG274520.18853899653601144No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT260520.17892386484613762No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC254680.17491298134121883No Hit
TTCAAGTAATTCAGGATAGGT251550.17276331261341135No Hit
TGAGGTAGTAGGTTGTGTGG241220.16566871901652588No Hit
TGAGATGAAGCACTGTAGCT237220.16292153853370478No Hit
TGTAAACATCCCCGACTGGAAG235100.1614655328778096No Hit
TGGCTCAGTTCAGCAGGAACAG225010.1545357701098934No Hit
TGAGGGGCAGAGAGCGAGACTT220130.15118420992085166No Hit
GTTCTATTTTGTTGGTTTTCGGAAC217990.14971446836254237No Hit
CTAGACTGAAGCTCCTTGAGG212200.14573792461365886No Hit
TGTAAACATCCTTGACTGGAAGCT211900.14553188607744727No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG203570.13981088272197237No Hit
TATTGCACTTGTCCCGGCCTG202280.13892491701626256No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT196910.13523682721807523No Hit
GTCTGAGCGTCGCT192340.13209817351645214No Hit
AGCTCGGTCTGAGGCCCCTCAGT185930.12769581679273134No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC185760.12757906162221144No Hit
TGAAAAGAACTTTGAAGAGAG183190.1258139981619989No Hit
TCAGTGCATCACAGAACTTTGT177970.12222892763191738No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT177320.12178251080345893No Hit
AAACCGTTACCATTACTGAGA177260.12174130309621663No Hit
TAGCAGCACGTAAATATTGGCG165480.11365085657430853No Hit
TGAGATGAAGCACTGTAGCTC162940.11190639696771712No Hit
CAACGGAATCCCAAAAGCAGCTG161610.1109929594571791No Hit
TGTCAGTTTGTCAAATACCCCAT160020.10990095521525774No Hit
TGTCAGTTTGTCAAATACCCCAA159340.10943393453317814No Hit
GGCTGGTCCGATGGTAGT158180.10863725219316003No Hit
TGAGGTAGTAGATTGTATAG157050.10786117370676306No Hit
GGGTGCTGTAGGCTT155600.10686532078174042No Hit
CGCGACCTCAGATCAGACG153460.10539557922343114No Hit
TGAGGTAGTAGGTTGTATAGTC151440.1040082530796065No Hit
AGACCGTTACCATTACTGAGT149800.10288190908164985No Hit
CGGGGCGCGGCCGGA148910.10227066142422216No Hit
TCCTGTACTGAGCTGCCCCGAA147360.10120612898712898No Hit
GCATTGTGGTTCAGTGGTAGAATTCTC146850.1008558634755693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)