FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13800300
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11232388.13922885734368No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3819582.7677514256936444No Hit
TTCAAGTAATCCAGGATAGGCT2981242.1602718781475767No Hit
TCAGTGCACTACAGAACTTTGT2964962.148475033151453No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2748161.991376999050745No Hit
CGCGACCTCAGATCAGA2373151.71963652964066No Hit
TATTGCACTTGTCCCGGCCTGT2184121.5826612464946415No Hit
TAGCTTATCAGACTGATGTTGAC1910081.3840858532060896No Hit
TGGAGTGTGACAATGGTGTTT1893151.3718180039564356No Hit
TCCTGTACTGAGCTGCCCCGAG1795951.3013847525053803No Hit
AAACCGTTACCATTACTGAGC1782371.2915443867162308No Hit
TCCTGTACTGAGCTGCCCCGA1644021.1912929429070382No Hit
TGAGGTAGTAGGTTGTATAGTT1514721.0975993275508502No Hit
TGAGGGGCAGAGAGCGAGACTTT1429351.035738353514054No Hit
TGAGGTAGTAGGTTGTATAGT1378860.9991521923436447No Hit
TGAGGTAGTAGGTTGTGTGGT1336040.9681238813648978No Hit
TGAGGTAGTAGATTGTATAGTT1301200.9428780533756512No Hit
TGAGGTAGTAGTTTGTGCTGTT1284170.9305377419331464No Hit
TGAGGTAGTAGGTTGTGTGGTT1231160.8921255335028948No Hit
AAGCTGCCAGTTGAAGAACTGT1006620.7294189256755288No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1003770.7273537531792786No Hit
TGAGGTAGTAGATTGTATAGT888040.6434932573929553No Hit
TAGCTTATCAGACTGATGTTGA769980.5579443925132062No Hit
TGAGGGGCAGAGAGCGAGACT745920.5405099889132845No Hit
TCGTACCGTGAGTAATAATGCG741780.5375100541292581No Hit
TGAGGTAGTAGTTTGTACAGT687590.4982427918233661No Hit
CGCGACCTCAGATC655920.47529401534749244No Hit
TCCTGTACTGAGCTGCCCCGAGA645270.4675767918088737No Hit
TGTAAACATCCCCGACTGGAAGCT637920.462250820634334No Hit
TTCAAGTAATCCAGGATAGGCC617250.4472728853720571No Hit
CGCGACCTCAGATCAGACGT611040.44277298319601743No Hit
CATTGCACTTGTCTCGGTCTGA582500.4220922733563763No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT573250.41538952051766986No Hit
TCCTGTACTGAGCTGCCCCGAGT571490.41411418592349447No Hit
CCCATAAAGTAGAAAGCACT570690.4135344883806874No Hit
TCCTGTACTGAGCTGCCCCG550740.3990782809069368No Hit
TCAGTGCACTACAGAACTTTGC483650.3504633957232814No Hit
TATTGCACTTGTCCCGGCCTGC475460.3445287421287943No Hit
TAGCTTATCAGACTGATGTTG445010.32246400440570133No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC434600.3149206901299247No Hit
AAACCGTTACCATTACTGAG400780.2904139765077571No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC390020.2826170445570024No Hit
TGAGAACTGAATTCCATGGGTT389580.2822982109084585No Hit
TGAGGTAGTAGGTTGTGTGG380070.2754070563683398No Hit
TGGCTCAGTTCAGCAGGAAC376300.2726752316978616No Hit
CTAGACTGAAGCTCCTTGAGG360180.2609943262102998No Hit
TGAGATGAAGCACTGTAGCTC329300.23861800105794803No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC328860.23829916740940416No Hit
TGAGGTAGTAGGTTGTATAG316550.22937907146946082No Hit
TGAGGTAGTAGTTTGTACAGTT306440.22205314377223684No Hit
AGCTCGGTCTGAGGCCCCTCAGT295330.21400259414650408No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT291690.21136497032673202No Hit
AAAAGCTGGGTTGAGAGGGCGA284930.20646652609001256No Hit
TATTGCACTTGTCCCGGCCTG284750.20633609414288095No Hit
TTCACAGTGGCTAAGTTCCG280790.2034665913059861No Hit
TGTAAACATCCCCGACTGGAAGC275180.19940146228705175No Hit
TGTAAACATCCTTGACTGGAAGCT270480.19599573922306035No Hit
CGCGACCTCAGATCAGAC263550.19097410925849437No Hit
TGAGGTAGTAGGTTGTGTGGC261340.18937269479648994No Hit
TGGAGTGTGACAATGGTGTTC253920.18399600008695463No Hit
TGAGGTAGTAGTTTGTGCTGT252920.18327137815844582No Hit
TGAGGTAGTAGGTTGTATAGTC240730.17443823684992354No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT240410.17420635783280072No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG237690.17223538618725678No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT227110.1645688861836337No Hit
TGAGGTAGTAGGTTGTGTGGTC225410.1633370289051687No Hit
TGAGATGAAGCACTGTAGCT223830.16219212625812482No Hit
TAGCTTATCAGACTGATGTTGACT223780.1621558951616994No Hit
CATTGCACTTGTCTCGGTCTG221400.16043129497184844No Hit
TTCAAGTAATTCAGGATAGGT220300.15963421085048876No Hit
AAACCGTTACCATTACTG220000.1594168242719361No Hit
TGAGGTAGTAGGTTGTATAGC214040.15509807757802366No Hit
TGAGGGGCAGAGAGCGAGACTT211380.15317058324819027No Hit
TGAGGTAGTAGGTTGTGTGGTTT211330.15313435215176482No Hit
AACCCGTAGATCCGATCTTGT209490.1518010478033086No Hit
TGAGGTAGTAGTTTGTGCTGTC208970.15142424440048405No Hit
CAACGGAATCCCAAAAGCAGCTG208000.1507213611298305No Hit
TGAGGTAGTAGATTGTATAGTC199860.14482293863176887No Hit
TGTAAACATCCCCGACTGGAAG198700.14398237719469867No Hit
TGGAGTGTGACAATGGTGTTTG192440.13944624392223356No Hit
AAGCTGCCAGTTGAAGAACT192210.13927958087867653No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT189790.13752599581168526No Hit
GGCTGGTCCGATGGTAGTGGGTT189520.13733034789098789No Hit
TGAGGTAGTAGGTTGTAT189180.13708397643529488No Hit
TCAGTGCATCACAGAACTTTGT185880.13469272407121585No Hit
TACCCTGTAGAACCGAATTTGT183070.13265653645210612No Hit
AAGCTGCCAGTTGAAGAACTGC180070.1304826706665797No Hit
TGAGGTAGTAGATTGTAT179470.13004789750947443No Hit
TGAGGTAGTAGATTGTATAGTTT179460.13004065129018935No Hit
TACAGTACTGTGATAACTGAAG167920.12167851423519778No Hit
TGGCTCAGTTCAGCAGGAACAG166120.12037419476388196No Hit
TTCAAGTAATCCAGGATAGGCA164620.11928726187111875No Hit
GGCTGGTCCGATGGTAGTGGGT160630.11639602037636863No Hit
GCATTGGTGGTTCAGTGGTAGAATT158790.11506271602791243No Hit
TGAGGGGCAGAGAGCGAGACTTC157590.11419316971370189No Hit
CAACGGAATCCCAAAAGCAGCT154600.11202655014746056No Hit
TAGCTTATCAGACTGATGTTGAT152870.11077295421114033No Hit
TGAGGTAGTAGATTGTATAG151610.10985993058121925No Hit
TCGTACCGTGAGTAATAATGC149160.10808460685637268No Hit
AAATGGATTTTTGGAGCAGGGAGA148090.10730926139286827No Hit
AGAGGTAGTAGGTTGCATAGTT147940.10720056810359195No Hit
CTAGACTGAAGCTCCTTGAGGA147600.10695419664789896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT147400.10680927226219719No Hit
AAACCGTTACCATTACTGAGTT144720.10486728549379361No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA142250.10307746933037687No Hit
TGAGGTAGTAGATTGTATAGC138140.1000992732042057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)