FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15526581
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT275241117.72709007862066No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC3828772.465945335937126No Hit
AAACCGTTACCATTACTGAGC3713042.3914086430232127No Hit
TCAGTGCACTACAGAACTTTGT3374142.1731377951140693No Hit
TGAGGTAGTAGGTTGTATAGT2842041.8304351743632419No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2712801.7471972741455444No Hit
TCCTGTACTGAGCTGCCCCGAG2295431.4783872895133836No Hit
TGAGGTAGTAGGTTGTGTGGT2148551.3837882274275322No Hit
TATTGCACTTGTCCCGGCCTGT1918991.2359385495106745No Hit
TGAGGTAGTAGGTTGTATAGTT1831581.179641545038151No Hit
TTCAAGTAATCCAGGATAGGCT1802371.1608286460489918No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1767961.138666651724549No Hit
TGAGGTAGTAGATTGTATAGT1721781.1089241089200512No Hit
TCCTGTACTGAGCTGCCCCGA1709131.1007767904601793No Hit
TGAGGTAGTAGGTTGTGTGGTT1449650.9336569332295371No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1260630.8119173177919853No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1218950.7850730305660982No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1214040.7819107117014364No Hit
TGAGGTAGTAGATTGTATAGTT1207880.7779433218427161No Hit
CGCGACCTCAGATCAGA1174870.7566830070316188No Hit
CATTGCACTTGTCTCGGTCTGA1124850.7244672861333735No Hit
TGAGGGGCAGAGAGCGAGACT1112740.7166677583429346No Hit
TGAGGTAGTAGTTTGTGCTGTT1042850.6716546289231351No Hit
AAACCGTTACCATTACTGAG977780.6297458532564252No Hit
TGAGGTAGTAGTTTGTACAGT948250.6107268560927869No Hit
AAGCTGCCAGTTGAAGAACTGT904650.582645979820026No Hit
TGAGGGGCAGAGAGCGAGACTTT891000.5738546045649071No Hit
TGGAGTGTGACAATGGTGTTT815210.5250415400531515No Hit
TCCTGTACTGAGCTGCCCCGAGA749040.48242430191167No Hit
TCGTACCGTGAGTAATAATGCG689500.444077160322675No Hit
TGAGGTAGTAGGTTGTATAG661570.4260886540314316No Hit
TAGCTTATCAGACTGATGTTGAC654560.4215738158967515No Hit
CCCATAAAGTAGAAAGCACT641900.41342005686892697No Hit
TCCTGTACTGAGCTGCCCCG575090.3703906223784876No Hit
TAGCTTATCAGACTGATGTTGA544240.35052147024512353No Hit
TCCTGTACTGAGCTGCCCCGAGT541370.34867302724276517No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT539660.34757169012289313No Hit
TGAGGTAGTAGGTTGTGTGG517310.3331770207491269No Hit
CGCGACCTCAGATCAGACGT507340.32675577450051624No Hit
TCAGTGCACTACAGAACTTTGC481220.3099330110086696No Hit
AAAAGCTGGGTTGAGAGGGCGA475150.3060235862615215No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT472080.3040463319001137No Hit
TGAGGTAGTAGTTTGTACAGTT470050.30273889660576275No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC423150.2725326329086874No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT396900.2556261420334586No Hit
TGAGGTAGTAGGTTGTATAGC396330.25525902966016795No Hit
CATTGCACTTGTCTCGGTCTG393420.25338482438599974No Hit
TTCAAGTAATTCAGGATAGGT385270.24813576150473823No Hit
CTAGACTGAAGCTCCTTGAGG372690.24003352702053338No Hit
AAACCGTTACCATTACTG368530.23735425075230662No Hit
TATTGCACTTGTCCCGGCCTGC358860.23112622154226997No Hit
TGAGGTAGTAGGTTGTGTGGC356310.2294838767143906No Hit
TAGCTTATCAGACTGATGTTG340630.21938506616492065No Hit
AAACCGTTACCATTACTGAGTT332300.2140200730605147No Hit
TTCAAGTAATCCAGGATAGGCC323920.2086228771163465No Hit
TTCACAGTGGCTAAGTTCCG311320.20050776149623667No Hit
TGGCTCAGTTCAGCAGGAAC289840.18667342153433522No Hit
TGAGGTAGTAGTTTGTGCTGT289460.18642867995214144No Hit
TGAGGTAGTAGATTGTATAG287230.18499243329874104No Hit
TGAGGTAGTAGTTTGTGCT277800.17891897771956364No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG277540.17875152295279947No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC271530.17488074161336614No Hit
TGTAAACATCCCCGACTGGAAGCT270070.17394041869230578No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC266890.17189231808342093No Hit
TGAGAACTGAATTCCATGGGTT262260.16891033512142822No Hit
TGAGGTAGTAGGTTGTATAGTC262160.16884592944190355No Hit
TGGCTCAGTTCAGCAGGAACAG258660.16659173065853972No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT258400.16642427589177552No Hit
AGAGGTAGTAGGTTGCATAGT243940.15711121463250666No Hit
AAACCGTTACTATTACTGAGT243830.15704036838502952No Hit
AAACCGTTACCATTACTGAGA243690.15695020043369495No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT240700.15502447061590702No Hit
TATTGCACTTGTCCCGGCCTG237960.15325975499693073No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA235080.15140487142661993No Hit
TGAGGTAGTAGATTGTATAGC233690.15050963248122687No Hit
TGAGGTAGTAGGTTGTAT231830.1493116868420678No Hit
TGAGGTAGTAGGTTGTGTGGTC230390.14838424505691242No Hit
AAATCGTTACCATTACTGAGT222560.14334128035012988No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA216690.13956066696203112No Hit
TGTAAACATCCTTGACTGGAAGCT214520.13816306371634554No Hit
TGAGGGGCAGAGAGCGAGACTT203860.13129741827901453No Hit
AAGCTGCCAGTTGAAGAACT195340.12581005438351173No Hit
TGAGATGAAGCACTGTAGCTC194260.12511447304464518No Hit
TCCTGTACTGAGCTGCCCCGAA192430.12393584910934351No Hit
AGACCGTTACCATTACTGAGT189340.12194571361203088No Hit
AAAAGCTGGGTTGAGAGGGCG187100.12050302639067803No Hit
CAACGGAATCCCAAAAGCAGCT186740.12027116594438918No Hit
TGTAAACATCCCCGACTGGAAG185020.11916338825656465No Hit
AGAGGTAGTAGGTTGCATAGTT184820.1190345768975153No Hit
AAACCGTTACCATTACTGAGG183320.11806849170464509No Hit
TGAGGGGCAGAGAGCGAGACC173810.11194351158184794No Hit
AAAACGTTACCATTACTGAGT173720.11188554647027571No Hit
TGAGGTAGTAGATTGTATAGTC171180.11024964221034882No Hit
TGAGGTAGTAGATTGTAT170170.10959914484714953No Hit
AAATGGATTTTTGGAGCAGGGAGA169690.10928999758543108No Hit
GAACCGTTACCATTACTGAGT169240.10900017202757001No Hit
AAAAGCTGGGTTGAGAGGGCGT167160.10766053389345664No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG163150.10507786614451695No Hit
TGAGATGAAGCACTGTAGCT162620.10473651604303612No Hit
AGCTCGGTCTGAGGCCCCTCAGT162410.10460126411603429No Hit
TACAGTATAGATGATGTACT161890.10426635458250597No Hit
TAGCAGCACGTAAATATTGGCG161820.1042212706068387No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT161440.10397652902464491No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG157210.1012521687807509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)