FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16211308
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11965647.381045378941662No Hit
TCAGTGCACTACAGAACTTTGT4349042.6827199878011077No Hit
TGAGGTAGTAGGTTGTATAGT2468571.5227457278586034No Hit
TTCAAGTAATCCAGGATAGGCT2417751.491397239507139No Hit
TGAGGTAGTAGGTTGTATAGTT2290051.412625063936852No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2274801.4032180500179257No Hit
AAACCGTTACCATTACTGAGC2200451.357355001829587No Hit
CGCGACCTCAGATCAGA2094381.291925364689882No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2062971.2725500002837526No Hit
TATTGCACTTGTCCCGGCCTGT1938081.195511182688035No Hit
TGAGGTAGTAGATTGTATAGTT1609780.9929982207481345No Hit
TCCTGTACTGAGCTGCCCCGA1507920.9301655363034247No Hit
TCCTGTACTGAGCTGCCCCGAG1428870.8814032772679417No Hit
TGAGGTAGTAGATTGTATAGT1413320.8718112073374955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1202080.7415071010926447No Hit
TGAGGTAGTAGGTTGTGTGGTT1124910.6939045263960194No Hit
AAGCTGCCAGTTGAAGAACTGT1098510.6776195973822716No Hit
TAGCTTATCAGACTGATGTTGAC1083290.6682310890644975No Hit
TGAGGTAGTAGGTTGTGTGGT1066880.658108525234361No Hit
TGAGGGGCAGAGAGCGAGACTTT1063550.6560544035064907No Hit
TGAGGTAGTAGTTTGTGCTGTT1047190.645962682344941No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT952120.5873184322942973No Hit
TCAGTGCACTACAGAACTTTGC852430.5258243196662478No Hit
CATTGCACTTGTCTCGGTCTGA833800.5143323413508645No Hit
TGAGGTAGTAGTTTGTACAGT730710.4507409272589232No Hit
TGAGGGGCAGAGAGCGAGACT705960.4354738063085347No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT702290.4332099544342751No Hit
CCCATAAAGTAGAAAGCACT653250.40295946508449537No Hit
TAGCTTATCAGACTGATGTTGA605290.37337517737618703No Hit
TCGTACCGTGAGTAATAATGCG588920.36327728768092No Hit
CGCGACCTCAGATCAGACGT580710.35821292149899314No Hit
TCCTGTACTGAGCTGCCCCGAGA557040.3436120021900762No Hit
TTCAAGTAATCCAGGATAGGCC538670.33228040575134343No Hit
TCCTGTACTGAGCTGCCCCGAGT538110.33193496786317306No Hit
TGGAGTGTGACAATGGTGTTT532920.3287334988638795No Hit
TGAGGTAGTAGTTTGTACAGTT505030.3115294583262498No Hit
TGGCTCAGTTCAGCAGGAAC467220.2882062323410301No Hit
TGAGGTAGTAGGTTGTATAGC465680.2872562781485615No Hit
TGAGGTAGTAGGTTGTATAGTC463180.28571414471922935No Hit
TATTGCACTTGTCCCGGCCTGC442650.2730501449975536No Hit
CGCGACCTCAGATC433720.26754164438797906No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC430220.26538265758691404No Hit
TCCTGTACTGAGCTGCCCCG423080.2609783245127414No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC409160.25239172557821987No Hit
TGAGGTAGTAGGTTGTATAG397000.24489078857794816No Hit
TTCACAGTGGCTAAGTTCCG396400.24452067655490847No Hit
AAAAGCTGGGTTGAGAGGGCGA389660.24036308482942892No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC382470.23592790908666963No Hit
AAACCGTTACCATTACTGAG363100.22397945927620397No Hit
TGTAAACATCCCCGACTGGAAGCT335000.20664587953051045No Hit
TGAGGTAGTAGGTTGTGTGG326960.20168637842177817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT324220.19999620018323014No Hit
TGAGGTAGTAGATTGTATAGTC323900.19979880710427558No Hit
TGAGATGAAGCACTGTAGCT318960.19675155144791523No Hit
TAGCTTATCAGACTGATGTTG303750.18736921166385834No Hit
TGAGGGGCAGAGAGCGAGACTT296140.18267495750497123No Hit
TGAGAACTGAATTCCATGGGTT294300.18153994730098275No Hit
TGAGGTAGTAGTTTGTGCTGT286420.17667914273172775No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT286120.17649408672020792No Hit
TAGCTTATCAGACTGATGTTGAT276340.17046126074466045No Hit
TGAGGTAGTAGGTTGTAT271690.16759289256610263No Hit
TGAGGTAGTAGATTGTATAGC265660.16387326673455344No Hit
CATTGCACTTGTCTCGGTCTG264870.16338595257088448No Hit
TTCAAGTAATTCAGGATAGGT263660.1626395599910877No Hit
AAACCGTTACCATTACTGAGA258050.15917901257566633No Hit
GGGTGCTGTAGGCTT254950.15726676712329443No Hit
TGAGGTAGTAGGTTGTGTGGTC242650.14967947065098017No Hit
CTAGACTGAAGCTCCTTGAGG242140.14936487543139643No Hit
AAACCGTTACCATTACTGAGTT235080.14500989062696237No Hit
TTCAAGTAATCCAGGATAGGCA234490.14464594713763998No Hit
AGGAAACTCTGGTG230200.14199964617290597No Hit
TGAGATGAAGCACTGTAGCTC229790.1417467362904955No Hit
TGTAAACATCCCCGACTGGAAG229260.1414198040034771No Hit
AGCTCGGTCTGAGGCCCCTCAGT228310.14083379330033086No Hit
AAACCGTTACCATTACTG228090.14069808555854962No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT226580.139766636967233No Hit
TGTAAACATCCTTGACTGGAAGCT224270.13834170567853007No Hit
AAGCTGCCAGTTGAAGAACTGC221890.13687359465380586No Hit
TGAGGTAGTAGGTTGTGTGGC218690.13489966386426067No Hit
AGAGGTAGTAGGTTGCATAGTT216210.13336986750236318No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT216050.1332711709628859No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT215670.13303676668162742No Hit
TGAGGTAGTAGTTTGTGCTGTC211960.1307482406724985No Hit
CAACGGAATCCCAAAAGCAGCT205860.126985435104928No Hit
AGAGGTAGTAGGTTGCATAGT204990.1264487726715204No Hit
TGGCTCAGTTCAGCAGGAACAG203710.12565920035570233No Hit
TCCTGTACTGAGCTGCCCCGAA203560.1255666723499424No Hit
TCAGTGCATCACAGAACTTTGT203100.12528291979894526No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG200050.12340151701516003No Hit
GCATTGGTGGTTCAGTGGTAGAATT199640.12314860713274955No Hit
TGAGGTAGTAGATTGTAT198330.12234052921577951No Hit
TGAGGTAGTAGATTGTATAG185260.11427825564723093No Hit
TGAGGGGCAGAGAGCGAGACTTC174840.10785064351377445No Hit
TGAAAAGAACTTTGAAGAGAG169150.10434074782861445No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT166880.10294049067478084No Hit
GGCTGGTCCGATGGTAGT165620.10216325542639743No Hit
GGAATACCGGGTGCTGTAGGCTT164340.10137368311057937No Hit
CAACGGAATCCCAAAAGCAGCTG163850.10107142495843024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)