FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11171129
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6423635.750206626384853No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6404035.732661398861297No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT4941074.423071293868328No Hit
TCAGTGCACTACAGAACTTTGT2599662.327123784892288No Hit
CGCGACCTCAGATCAGA2194551.964483625603106No Hit
TTCAAGTAATCCAGGATAGGCT1579201.4136440461836937No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1482661.3272248489834824No Hit
TAGCTTATCAGACTGATGTTGAC1406211.2587895099949165No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1402541.2555042556575973No Hit
TCCTGTACTGAGCTGCCCCGA1327021.1879014198117308No Hit
TGAGGTAGTAGGTTGTATAGTT1136651.0174889216658407No Hit
TCCTGTACTGAGCTGCCCCGAG1022900.9156639405023431No Hit
TGAGGGGCAGAGAGCGAGACTTT887990.7948972749307612No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC826110.7395044851778186No Hit
TGAGGTAGTAGATTGTATAGTT824410.737982705239551No Hit
AAACCGTTACCATTACTGAGC787830.7052375816267094No Hit
TCGTACCGTGAGTAATAATGCG774280.6931081003540466No Hit
TATTGCACTTGTCCCGGCCTGT744810.666727597541842No Hit
TGGAGTGTGACAATGGTGTTT692590.619982098496938No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC675520.6047016375873916No Hit
AAGCTGCCAGTTGAAGAACTGT669210.5990531485224099No Hit
TGAGGTAGTAGGTTGTATAGT665670.5958842655921348No Hit
AAATGGATTTTTGGAGCAGGGAGA641490.5742391838819514No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT614390.5499802213366258No Hit
AAATGGATTTTTGGAAATAGGAGA605690.5421922887113737No Hit
TAGCTTATCAGACTGATGTTGA562530.5035569815727667No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT547150.4897893489547923No Hit
TGGCTCAGTTCAGCAGGAAC523880.4689588670939169No Hit
TGAGGTAGTAGGTTGTGTGGTT516280.4621556156051908No Hit
AAAAGCTGGGTTGAGAGGGCGA498230.44599789331946665No Hit
TGAGGGGCAGAGAGCGAGACT477770.4276828241800807No Hit
TTCACAGTGGCTAAGTTCCG476260.42633112552903113No Hit
TGAGGTAGTAGTTTGTGCTGTT449830.402671923312317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT440990.3947586676333252No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT407540.36481540943623514No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA395010.35359899612653295No Hit
TGAGGTAGTAGGTTGTGTGGT385260.34487114059823315No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC375700.33631336635715153No Hit
TGAGGTAGTAGATTGTATAGT373560.3343977139642735No Hit
TCAGTGCACTACAGAACTTTGC345080.3089034241749424No Hit
TAGCTTATCAGACTGATGTTG345040.3088676175881596No Hit
TCCTGTACTGAGCTGCCCCG340650.3049378446887508No Hit
CTAGACTGAAGCTCCTTGAGG321680.2879565709070229No Hit
AAATGGATTTTTGGAAATAGGAG320550.28694503483040973No Hit
TGAGGTAGTAGGTTGTGTGG315980.28285413229047845No Hit
TCCTGTACTGAGCTGCCCCGAGA305890.2738219207745251No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA299770.26834351299676157No Hit
AAATGGATTTTTGGAGCAGGGAG298580.2672782670399742No Hit
CGCGACCTCAGATCAGACGT293740.2629456700392592No Hit
CATTGCACTTGTCTCGGTCTGA287000.25691226016636276No Hit
CCCATAAAGTAGAAAGCACT283360.2536538607691308No Hit
AAACCGTTACCATTACTGAG262840.23528508174957072No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG260140.23286813714173382No Hit
TGTAAACATCCCCGACTGGAAGCT255370.22859820166788872No Hit
TTCAAGTAATCCAGGATAGGCC246050.2202552669475037No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC242830.21737283671149085No Hit
TGAGAACTGAATTCCATGGGTT236100.2113483784852901No Hit
TGGCTCAGTTCAGCAGGAACAG228310.20437504570934595No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT226920.20313076681864475No Hit
TGAGGTAGTAGGTTGTATAG220480.19736590634661902No Hit
TCCTGTACTGAGCTGCCCCGAGT217950.19510113973260895No Hit
TGAGGGGCAGAGAGCGAGACTT214060.19161894916798472No Hit
TGTAAACATCCCCGACTGGAAG212510.19023144393015246No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA211940.189721200068498No Hit
AAACCGTTACCATTACTG201470.1803488259781084No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC201410.18029511609793425No Hit
TGAGGTAGTAGTTTGTACAGT195090.17463767538625685No Hit
TGTAAACATCCCCGACTGGAAGC191540.17145984080928614No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT185420.1659814330315226No Hit
CGCGACCTCAGATC173790.1555706679244327No Hit
AAATGGATTTTTGGAGCAGGG164660.14739781449126585No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG158120.1415434375522832No Hit
CGCGACCTCAGATCAGAC156980.14052294982897431No Hit
AAATGGATTTTTGGAGCAGGGA156590.14017383560784233No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGT156050.13969044668627495No Hit
TGAGATGAAGCACTGTAGCTC150720.13491921899747109No Hit
CAACGGAATCCCAAAAGCAGCTG146640.1312669471456287No Hit
TGGAGTGTGACAATGGTGTTTG146510.1311505757385847No Hit
AAAAGCTGGGTTGAGAGGGCG145670.13039863741614657No Hit
TGAGGTAGTAGTTTGTACAGTT142010.12712233472552328No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC140750.12599442724186605No Hit
AGAAGCCGGCGGGAGC140280.12557369984716854No Hit
AGCTCGGTCTGAGGCCCCTCAGT138830.12427571107629318No Hit
TACAGTACTGTGATAACTGAAG137540.1231209486525489No Hit
AAATGGATTTTTGGAGCAGGGAGAT136230.12194828293541325No Hit
TCGTACCGTGAGTAATAATGC135230.12105311826584404No Hit
TGGAGAGAAAGGCAGTTCCTGA134250.1201758568896662No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA134030.11997892066236099No Hit
TGAGGTAGTAGTTTGTGCTGT130420.11674737620521614No Hit
GGCTGGTCCGATGGTAGTGGGTT130370.11670261797173768No Hit
TGTAAACATCCTTGACTGGAAGCT130270.11661310150478076No Hit
TGAGGTAGTAGGTTGTATAGTC128520.11504656333303466No Hit
CATTGCACTTGTCTCGGTCTG127540.11416930195685682No Hit
CTAGACTGAAGCTCCTTGAGGA126860.11356058998154975No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC126200.11296978129963409No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC125440.11228945615076148No Hit
GGCTGGTCCGATGGTAGTGGGTTATC124770.1116896958221501No Hit
TGAGGTAGTAGGTTGTGTGGTTT121430.10869984582578895No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC121110.10841339313152681No Hit
AAAAGCTGGGTTGAGAGGGCGAA117240.10494910586029399No Hit
TATTGCACTTGTCCCGGCCTG116590.104367248825074No Hit
TAGCTTATCAGACTGATGTTGACT116580.10435829717837829No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT116160.10398232801715923No Hit
GGCTGGTCCGATGGTAGTG116160.10398232801715923No Hit
AACATTCAACGCTGTCGGTGA114600.10258587113263126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)