FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10718348
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC7384216.889317271654176No Hit
AAACCGTTACCATTACTGAGT4434254.1370647790125865No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3569503.33027067230883No Hit
TCAGTGCACTACAGAACTTTGT1788371.6685127222963836No Hit
TCCTGTACTGAGCTGCCCCGAG1714501.5995935194490793No Hit
TAGCTTATCAGACTGATGTTGAC1579581.4737159121909458No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1404511.3103791741040691No Hit
TCCTGTACTGAGCTGCCCCGA1375891.2836772980313758No Hit
TTCAAGTAATCCAGGATAGGCT1306191.2186486201045161No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1013680.9457427581190683No Hit
TGAGGGGCAGAGAGCGAGACTTT936900.8741085846438277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC917770.8562606849488372No Hit
TGAGGTAGTAGGTTGTATAGTT872370.8139034112346417No Hit
TGAGGTAGTAGGTTGTATAGT858860.8012988568760784No Hit
TATTGCACTTGTCCCGGCCTGT745080.6951444383033655No Hit
TGAGGTAGTAGTTTGTGCTGTT704720.6574893817592039No Hit
TAGCTTATCAGACTGATGTTGA676710.6313566232408203No Hit
AAGCTGCCAGTTGAAGAACTGT676350.6310207505858179No Hit
TGAGGTAGTAGATTGTATAGTT661890.6175298656098869No Hit
TGAGGTAGTAGGTTGTGTGGTT606380.5657401681677064No Hit
TCCTGTACTGAGCTGCCCCGAGA588970.5494969933799501No Hit
AAACCGTTACCATTACTGAGC577690.5389729835232071No Hit
TGGCTCAGTTCAGCAGGAAC532400.4967183375647068No Hit
TGAGGTAGTAGGTTGTGTGGT521550.48659550893477244No Hit
TCGTACCGTGAGTAATAATGCG515920.4813428338023733No Hit
CGCGACCTCAGATCAGA498190.46480110554350357No Hit
TGAGGTAGTAGATTGTATAGT496690.46340163614766006No Hit
CATTGCACTTGTCTCGGTCTGA464920.4337608743436955No Hit
TCCTGTACTGAGCTGCCCCG457980.42728599593892636No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC455270.42475762123043587No Hit
TTCACAGTGGCTAAGTTCCG439100.40967134114324333No Hit
AAACCGTTACCATTACTGAG434210.40510907091279363No Hit
TGAGGGGCAGAGAGCGAGACT424700.3962364349431461No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT417810.38980820551823847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT405250.3780899817770425No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA394740.36828436621016597No Hit
AAAAGCTGGGTTGAGAGGGCGA372950.3479547407865466No Hit
TCCTGTACTGAGCTGCCCCGAGT365930.34140522401399914No Hit
TAGCTTATCAGACTGATGTTG361220.33701089011105073No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT347870.3245556124880439No Hit
TGAGGTAGTAGTTTGTACAGT345050.32192461002385814No Hit
TGGAGTGTGACAATGGTGTTT333440.31109271690002976No Hit
AAATGGATTTTTGGAGCAGGGAGA331360.30915211933779346No Hit
CCCATAAAGTAGAAAGCACT310260.28946624983626207No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC287150.26790509134430046No Hit
TGTAAACATCCCCGACTGGAAGCT284120.2650781631646967No Hit
CAACGGAATCCCAAAAGCAGCTG277830.2592097214981264No Hit
TGTAAACATCCCCGACTGGAAG274240.2558603247440744No Hit
TGAGGTAGTAGGTTGTGTGG273530.2551979092300418No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC271760.2535465353429465No Hit
CATTGCACTTGTCTCGGTCTG267190.24928281858361007No Hit
AAATGGATTTTTGGAAATAGGAGA266840.24895627572457993No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA262490.24489781447663392No Hit
TCAGTGCACTACAGAACTTTGC247860.23124832296917397No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT246890.2303433327598619No Hit
TGAGGTAGTAGGTTGTATAG241880.22566910497774473No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA235620.2198286526990913No Hit
CTAGACTGAAGCTCCTTGAGG228530.21321382735473787No Hit
TGAGAACTGAATTCCATGGGTT226630.21144116612000283No Hit
TACAGTACTGTGATAACTGAAG224290.20925799386248703No Hit
AAATGGATTTTTGGAGCAGGGAG218170.2035481587274457No Hit
CGCGACCTCAGATCAGACGT215300.20087050728339853No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG214420.20004948523783703No Hit
TTCAAGTAATCCAGGATAGGCC209120.19510469337252345No Hit
TGTAAACATCCTTGACTGGAAGCT196610.18343311861118897No Hit
TGAGGGGCAGAGAGCGAGACTT193140.18019567940880443No Hit
TGTAAACATCCCCGACTGGAAGC192910.17998109410144175No Hit
AAACCGTTACCATTACTG176350.16453095197132991No Hit
TGGCTCAGTTCAGCAGGAACAG172510.16094831031797063No Hit
TGAGGTAGTAGTTTGTACAGTT171160.15968878786171153No Hit
AGAAGCCGGCGGGAGC170470.15904503193962355No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC169500.15814004173031143No Hit
TGAGATGAAGCACTGTAGCTC167900.15664727437474507No Hit
CGCGACCTCAGATC166070.15493992171181603No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA160840.1500604384183085No Hit
AAATGGATTTTTGGAGCAGGGA160200.14946333147608193No Hit
GTTTCCGTAGTGTAGTGGTTATC158150.14755072330176255No Hit
GGCTGGTCCGATGGTAGTGGGTTATC157600.1470375845232866No Hit
AAATGGATTTTTGGAAATAGGAG156770.14626321145758656No Hit
AAACCGTTACCATTACTGAGTT155190.14478910369396478No Hit
AACATTCAACGCTGTCGGTGA147310.13743722446780043No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT147040.13718531997654862No Hit
AAATGGATTTTTGGAGCAGGG146810.13697073466918597No Hit
AGCTCGGTCTGAGGCCCCTCAGT145630.13586981874445578No Hit
TATTGCACTTGTCCCGGCCTG143140.13354669954735562No Hit
TAGCAGCACGTAAATATTGGCG142010.13249243260248686No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG138880.12957220646316017No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA133880.1249073084770153No Hit
CAACGGAATCCCAAAAGCAGCT132810.12390902030798029No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC129920.12121270927198854No Hit
TGAGGTAGTAGTTTGTGCTGT128150.11956133538489327No Hit
AAAAGCTGGGTTGAGAGGGCG128080.11949602681308724No Hit
AGCAGCATTGTACAGGGCTATG123600.11531627821750143No Hit
TGAGGTAGTAGATTGTATAG121350.11321707412373623No Hit
TCAGTGCATCACAGAACTTTGT118830.11086596553871922No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC118830.11086596553871922No Hit
TTCAAGTAATTCAGGATAGGT117770.1098770071656565No Hit
TGGAGAGAAAGGCAGTTCCTG115890.10812300552286602No Hit
TACAGTACTGTGATAACTGA113310.10571591816201527No Hit
TGAGATGAAGCACTGTAGCT112640.10509082183187185No Hit
TACAGTATAGATGATGTACT112520.10497886428020436No Hit
TGAGGTAGTAGGTTGTATAGC111890.10439108713395011No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC111440.1039712463151971No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC109850.10248780875560301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)