FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12330211
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT161433013.092476681867002No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5034374.082955271406142No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4697983.8101375556346926No Hit
AAACCGTTACCATTACTGAGC3032762.459617276622436No Hit
TCCTGTACTGAGCTGCCCCGA2660652.157830064708544No Hit
TCCTGTACTGAGCTGCCCCGAG2505152.0317170565856495No Hit
TATTGCACTTGTCCCGGCCTGT1980791.6064526389694387No Hit
TCAGTGCACTACAGAACTTTGT1544691.2527685049347492No Hit
CGCGACCTCAGATCAGA1490371.2087141087853241No Hit
TTCAAGTAATCCAGGATAGGCT1045580.8479822445860821No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1005310.8153226250548349No Hit
AAGCTGCCAGTTGAAGAACTGT955130.7746258356811574No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT867070.7032077553255172No Hit
TGAGGGGCAGAGAGCGAGACTTT785070.6367044327140874No Hit
AAACCGTTACCATTACTGAG748970.6074267504424701No Hit
TCCTGTACTGAGCTGCCCCG698730.5666813001010282No Hit
TCCTGTACTGAGCTGCCCCGAGA697980.5660730380039726No Hit
TGAGGGGCAGAGAGCGAGACT688070.5580358681615425No Hit
TAGCTTATCAGACTGATGTTGAC666590.5406152417018655No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC637850.5173066381426887No Hit
CATTGCACTTGTCTCGGTCTGA590070.4785562874795897No Hit
TCCTGTACTGAGCTGCCCCGAGT532040.4314930214900621No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC524130.42507788390644735No Hit
TGAGGTAGTAGGTTGTGTGGTT511010.41443735228861855No Hit
TGAGGTAGTAGTTTGTGCTGTT510770.4142427084175607No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT506150.41049581389969725No Hit
TATTGCACTTGTCCCGGCCTGC494400.400966374379157No Hit
TGAGGTAGTAGGTTGTGTGGT488870.39648145518353256No Hit
TGGAGTGTGACAATGGTGTTT486690.39471344002142383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT482580.39138016372955825No Hit
TAGCTTATCAGACTGATGTTGA466440.37829036340091826No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG449060.3641949030718128No Hit
AAAAGCTGGGTTGAGAGGGCGA439500.35644158887467536No Hit
CGCGACCTCAGATC438860.35592253855185446No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT381700.30956485659491145No Hit
TGTAAACATCCCCGACTGGAAGCT379670.30791849385221387No Hit
CGCGACCTCAGATCAGACGT378530.3069939354646891No Hit
TGAGGTAGTAGTTTGTACAGT372720.3022819317528305No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT371070.3009437551393079No Hit
CCCATAAAGTAGAAAGCACT369260.29947581594507994No Hit
TGAGGTAGTAGGTTGTATAGTT368830.29912707900943464No Hit
AAACCGTTACCATTACTGAGTT338540.27456140044967603No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA334980.27167418302898466No Hit
TAGCTTATCAGACTGATGTTG333920.27081450593181255No Hit
TGAGGTAGTAGATTGTATAGTT329180.2669702894784201No Hit
TCAGTGCACTACAGAACTTTGC308370.2500930438254463No Hit
TGAGGTAGTAGTTTGTGCTGT297470.24125296801490256No Hit
TGAGGTAGTAGGTTGTATAGT281530.22832537091214417No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT280730.227676558008618No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT277160.22478123042663262No Hit
TGTAAACATCCTTGACTGGAAGCT272250.2207991412312409No Hit
AAACCGTTACCATTACTG267640.21706035687467148No Hit
TCCTGTACTGAGCTGCCCCGAA253180.20533306364343643No Hit
TTCAAGTAATCCAGGATAGGCC247960.20109955944792834No Hit
CATTGCACTTGTCTCGGTCTG235880.19130248460468355No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT232170.18829361476458106No Hit
TATTGCACTTGTCCCGGCCTG226640.1838086955689566No Hit
AAGCTGCCAGTTGAAGAACT226220.1834680687946054No Hit
TCGTACCGTGAGTAATAATGCG219860.17831000621157253No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA212660.17247069007983723No Hit
TGAGGTAGTAGATTGTATAGT211650.1716515637891355No Hit
TGAGAACTGAATTCCATGGGTT211350.1714082589503132No Hit
TGGCTCAGTTCAGCAGGAAC203790.16527697701199112No Hit
TGAGGTAGTAGGTTGTGTGG202990.16462816410846495No Hit
TGAGGGGCAGAGAGCGAGACTT202970.16461194378587682No Hit
TGAGGTAGTAGGTTGTATAG201430.163362978946589No Hit
TGTAAACATCCCCGACTGGAAG199840.16207346330083078No Hit
AAGCTGCCAGTTGAAGAACTGC198350.16086504926801334No Hit
AAACCGTTACCATTACTGAGA195080.15821302652485023No Hit
TCCTGTACTGAGCTGCCCCA180620.14648573329361517No Hit
TACAGTACTGTGATAACTGAAG178180.14450685393786045No Hit
CGCGACCTCAGATCAGAC178180.14450685393786045No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC174360.14140877232352309No Hit
TGAGGTAGTAGTTTGTACAGTT169900.13779164038636482No Hit
GCATTGGTGGTTCAGTGGTAGAATT161620.13107642683486925No Hit
ACCAATATTACTGTGCTGCTT154620.1253993139290155No Hit
TGAGGGGCAGAGAGCGAGACC145120.11769466069964252No Hit
AAAAGCTGGGTTGAGAGGGCG142900.1158942048923575No Hit
AAACCGTTACCATTACTGAGG142460.1155373577954181No Hit
AAAAGCTGGGTTGAGAGGGCGT142030.1151886208597728No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA137440.11146605682579155No Hit
CTAGACTGAAGCTCCTTGAGG135550.10993323634121102No Hit
AGCTCGGTCTGAGGCCCCTCAGT132930.10780837408216291No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC131050.10628366375887648No Hit
TTCAAGTAATTCAGGATAGGT130900.10616201133946532No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC126760.1028044045637175No Hit
TGTAAACATCCCCGACTGGAAGC125520.101798744563252No Hit
TGGAGAGAAAGGCAGTTCCTGA123840.1004362374658471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)