FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12116287
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7338726.056905056804943No Hit
CGCGACCTCAGATCAGA3952153.2618491126860896No Hit
CGCGACCTCAGATC2343561.9342229182917177No Hit
TCCTGTACTGAGCTGCCCCGAG2025361.671601209182318No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1922161.5864266008225127No Hit
TCAGTGCACTACAGAACTTTGT1792041.4790339647781536No Hit
TCCTGTACTGAGCTGCCCCGA1486201.2266134006234748No Hit
TATTGCACTTGTCCCGGCCTGT940730.7764177259914692No Hit
TTCAAGTAATCCAGGATAGGCT911650.7524169739458961No Hit
TGGAGTGTGACAATGGTGTTT889030.7337478882763341No Hit
CGCGACCTCAGATCAGACGT833390.6878262292730437No Hit
AAACCGTTACCATTACTGAGC797380.6581059032358676No Hit
TGAGGTAGTAGGTTGTATAGT765400.6317116786685558No Hit
AAACCGTTACCATTACTGAG713470.5888520138223863No Hit
TGAGGTAGTAGGTTGTATAGTT687070.5670631605210409No Hit
TAGCTTATCAGACTGATGTTGAC672770.555260864982812No Hit
CATTGCACTTGTCTCGGTCTGA661170.545686974895857No Hit
TCCTGTACTGAGCTGCCCCGAGA644260.5317305540880635No Hit
AAGCTGCCAGTTGAAGAACTGT634190.5234194270901638No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT600250.49540754523229763No Hit
TAGCTTATCAGACTGATGTTGA531620.43876478000232255No Hit
TGAGGTAGTAGATTGTATAGTT517690.4272678585444534No Hit
TGAGGTAGTAGATTGTATAGT508670.4198233336664937No Hit
TGAGGTAGTAGTTTGTGCTGTT483870.39935501692886605No Hit
TCCTGTACTGAGCTGCCCCG477190.39384177677534377No Hit
CGCGACCTCAGATCAGAC446970.36890014242812175No Hit
TGAGGGGCAGAGAGCGAGACTTT427020.35243470214926403No Hit
TGGCTCAGTTCAGCAGGAAC405820.33493759268000173No Hit
TGAGGTAGTAGGTTGTGTGGT394090.3252564089972448No Hit
TGAGGTAGTAGTTTGTACAGT379860.31351188693367865No Hit
TGAGGTAGTAGGTTGTGTGGTT378970.3127773384701105No Hit
AAAAGCTGGGTTGAGAGGGCGA369890.30528329347101135No Hit
CGCGACCTCAGATCAGACG361270.29816890273398117No Hit
TGAAAAGAACTTTGAAGAGAG354830.29285374306501655No Hit
TCGTACCGTGAGTAATAATGCG340350.2809028871633694No Hit
TAGCTTATCAGACTGATGTTG338340.27924396310519883No Hit
TCCTGTACTGAGCTGCCCCGAGT320860.26481710114658064No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC316430.26116086553578666No Hit
TGAGGTAGTAGGTTGTATAG304200.2510670141768679No Hit
CCCATAAAGTAGAAAGCACT279670.23082153798436766No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC277460.2289975468557323No Hit
AAACCGTTACCATTACTG273020.2253330578914151No Hit
TGAGGTAGTAGTTTGTACAGTT264480.21828469398257072No Hit
TGAGGGGCAGAGAGCGAGACT246650.20356896465063926No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT244890.20211637443054956No Hit
CATTGCACTTGTCTCGGTCTG226280.1867568835238056No Hit
AAACCGTTACCATTACTGAGTT224810.18554364055588976No Hit
TGTAAACATCCCCGACTGGAAG223040.18408279698227684No Hit
TTCACAGTGGCTAAGTTCCG212350.17525996206593653No Hit
TCAGTGCACTACAGAACTTTGC208810.17233827491871065No Hit
GTTTCCGTAGTGTAGTGGTTATC198240.1636144802446492No Hit
CAACGGAATCCCAAAAGCAGCTG186900.1542551773492985No Hit
GCATTGGTGGTTCAGTGGTAGAATT183220.15121794325274732No Hit
GGGTGCTGTAGGCTT174930.1443759131819839No Hit
TGTCTGAGCGTCGCT173630.1433029772239631No Hit
TGTAAACATCCCCGACTGGAAGCT169390.13980355533011063No Hit
TGAGGTAGTAGATTGTATAG168140.138771886139706No Hit
TGAGAACTGAATTCCATGGGTT167080.1378970306662429No Hit
GGCTGGTCCGATGGTAGTG161840.13357227342006672No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC160420.1324002972197671No Hit
AAATGGATTTTTGGAGCAGGGAGA159700.13180605576609403No Hit
TGAGATGAAGCACTGTAGCTC158220.13058455944465494No Hit
AACCCGTAGATCCGATCTTGT150640.12432851747404136No Hit
TGTAAACATCCCCGACTGGAAGC150630.12432026412051811No Hit
CAACGGAATCCCAAAAGCAGCT147650.12186076477059349No Hit
TGAGGTAGTAGGTTGTGTGG147020.12134080349862958No Hit
CTAGACTGAAGCTCCTTGAGG145960.12046594802516644No Hit
TGGCTCAGTTCAGCAGGAACAG145880.12039992119698055No Hit
GGCTGGTCCGATGGTAGT143540.11846863647254312No Hit
TATTGCACTTGTCCCGGCCTG143440.11838610293731075No Hit
AAATGGATTTTTGGAGCAGGGA140570.11601739047614175No Hit
CAGTCGGTCCTGAG139050.11476288074060972No Hit
GTTGAAAAGAACTTTGAAGAGAG137560.11353313106564741No Hit
TTCAAGTAATTCAGGATAGGT135290.11165961981587262No Hit
TGAGGTAGTAGTTTGTGCTGT135080.11148629939188466No Hit
GTCTGAGCGTCGCT131820.10879570614330941No Hit
AGCTCGGTCTGAGGCCCCTCAGT130210.10746691622606827No Hit
GGCTGGTCCGATGGTAGTGGGTTATC127380.10513121717899221No Hit
GGCTGGTCCGATGGTAGTGGGT125280.10339801293911244No Hit
TTCAAGTAATCCAGGATAGGCC124880.10306787879818297No Hit
TGTAAACATCCTTGACTGGAAGCT123560.10197843613311569No Hit
CCTGTCTGAGCGTCGCT122900.10143371480058207No Hit
GGCTGGTCCGATGGTAGTGGGTT122400.10102104712442021No Hit
AAATGGATTTTTGGAGCAGGG121600.10036077884256127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)