FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13170089
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT161640712.273318730040472No Hit
AAACCGTTACCATTACTGAGC3255912.4722004536188025No Hit
CGCGACCTCAGATCAGA2273161.7260020034792476No Hit
TTCAAGTAATCCAGGATAGGCT2161751.6414088014135668No Hit
TATTGCACTTGTCCCGGCCTGT1926121.4624958115317217No Hit
TCCTGTACTGAGCTGCCCCGAG1911131.4511139598221394No Hit
TGGAGTGTGACAATGGTGTTT1863991.4153207316974092No Hit
TCCTGTACTGAGCTGCCCCGA1629091.2369620281229687No Hit
TGAGGTAGTAGGTTGTATAGT1527891.1601212413978372No Hit
AAACCGTTACCATTACTGAGG1468031.1146697641906596No Hit
TCAGTGCACTACAGAACTTTGT1456971.1062719469853242No Hit
TAGCTTATCAGACTGATGTTGAC1323421.004867924582742No Hit
TGAGGTAGTAGGTTGTATAGTT1295060.9833342811882289No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1290240.9796744729667354No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1151070.8740032052934493No Hit
TGAGGTAGTAGATTGTATAGTT989310.7511794339430812No Hit
TGAGGTAGTAGATTGTATAGT946020.7183094966176766No Hit
AAACCGTTACCATTACTGAG924430.7019162892521076No Hit
TGAGGGGCAGAGAGCGAGACTTT783410.5948403234025221No Hit
AAGCTGCCAGTTGAAGAACTGT774770.5882800032710485No Hit
TGAGGTAGTAGTTTGTGCTGTT771650.5859109987791274No Hit
TGAGGTAGTAGGTTGTGTGGT764680.5806187034878807No Hit
TGAGGTAGTAGGTTGTGTGGTT690590.524362439767871No Hit
TCCTGTACTGAGCTGCCCCG674950.5124870454558051No Hit
TCGTACCGTGAGTAATAATGCG576980.4380987858168612No Hit
TGAGGTAGTAGTTTGTACAGT562030.42674730595973953No Hit
TGAGGGGCAGAGAGCGAGACT559570.42487943703341713No Hit
TCCTGTACTGAGCTGCCCCGAGA546900.41525915276654546No Hit
TAGCTTATCAGACTGATGTTGA540260.4102174252580981No Hit
TTCAAGTAATCCAGGATAGGCC525220.39879760873294023No Hit
TATTGCACTTGTCCCGGCCTGC494730.37564666419490406No Hit
TGTAAACATCCCCGACTGGAAGCT485730.36881299739128565No Hit
TGAGGTAGTAGGTTGTATAG474360.3601797983293811No Hit
AAACCGTTACCATTACTG461320.3502785744272495No Hit
CCCATAAAGTAGAAAGCACT455070.34553297248029224No Hit
TCCTGTACTGAGCTGCCCCGAGT452180.3433386061400192No Hit
TAGCTTATCAGACTGATGTTG402800.30584455427749957No Hit
TGGAGTGTGACAATGGTGTTC359530.2729898028783253No Hit
AAACCGTTACCATTACTGAGA357880.27173696396432856No Hit
CGCGACCTCAGATC349180.2651310860541641No Hit
TATTGCACTTGTCCCGGCCTG342700.2602108459555589No Hit
AAAAGCTGGGTTGAGAGGGCGA325880.24743948199590754No Hit
TCAGTGCACTACAGAACTTTGC313620.23813050921675624No Hit
TGAGGTAGTAGGTTGTATAGC310920.2360804091756707No Hit
TTCAAGTAATCCAGGATAGGCG306070.2323978220648319No Hit
TTCAAGTAATTCAGGATAGGT301550.22896580273679243No Hit
TGAGAACTGAATTCCATGGGTT300750.2283583656875819No Hit
TGAGGTAGTAGGTTGTGTGG296750.22532118044152927No Hit
TGAGGTAGTAGGTTGTATAGTC288940.21939107624861154No Hit
CGCGACCTCAGATCAGACGT287370.2181989810395359No Hit
TAGCTTATCAGACTGATGTTGAT272830.20715881267013456No Hit
CATTGCACTTGTCTCGGTCTGA267410.20304342666173325No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC259450.19699942802208853No Hit
TGGCTCAGTTCAGCAGGAAC254050.19289922793991748No Hit
TGAGGTAGTAGATTGTATAG253060.19214752459151946No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT246700.1873184000502958No Hit
TGAGGTAGTAGTTTGTACAGTT244280.18548090297643394No Hit
TTCAAGTAATCCAGGATAGGCA235110.1785181557998583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC228980.17386366941028264No Hit
TTCACAGTGGCTAAGTTCCG227110.17244378530775303No Hit
CTAGACTGAAGCTCCTTGAGG225530.17124409713556227No Hit
CAACGGAATCCCAAAAGCAGCT221490.1681765400370491No Hit
TGAGGTAGTAGGTTGTAT219340.1665440529672958No Hit
TGAGGTAGTAGATTGTATAGTC214970.16322592808598332No Hit
CGCGACCTCAGATCAGAC211240.16039375284403926No Hit
TCCTGTACTGAGCTGCCCCGAA210210.15961167764318068No Hit
TATTGCACTTGTCCCGGCCTGG209760.1592699943029998No Hit
AGCTCGGTCTGAGGCCCCTCAGT205660.15615687942579584No Hit
TGAGGGGCAGAGAGCGAGACTT205300.15588353275365108No Hit
TGTAAACATCCTTGACTGGAAGCT200560.15228446823707872No Hit
CGCGACCTCAGATCAGG197620.15005213708123005No Hit
AAACCGTTACCATTACTGAGTT196640.14930802669594717No Hit
TGTAAACATCCCCGACTGGAAGC195080.14812352444998664No Hit
TGTAAACATCCCCGACTGGAAG192750.14635436404416097No Hit
TACAGTACTGTGATAACTGAAG191490.1453976506916544No Hit
TGAGGTAGTAGATTGTATAGC188750.14331717879810835No Hit
TGAGGTAGTAGATTGTAT187740.14255028952348006No Hit
AAGCTGCCAGTTGAAGAACT184280.13992312428564455No Hit
CATTGCACTTGTCTCGGTCTG183230.13912586315855574No Hit
TGAGGTAGTAGTTTGTGCTGT183060.1389967827855985No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG181680.13794895387571035No Hit
TGAGGTAGTAGGTTGTGTGGC175510.13326409563367414No Hit
ACCAATATTACTGTGCTGCTT173180.1314949352278485No Hit
AAGCTGCCAGTTGAAGAACTGC172610.131062136330286No Hit
AACCCGTAGATCCGATCTTGT172110.13068248817452943No Hit
TGAGGTAGTAGTTTGTGCTGTC168990.12831348368260836No Hit
TGAGGTAGTAGGTTGTGTGGTC160050.12152537465768075No Hit
TACAGTATAGATGATGTACT157470.1195663901739768No Hit
TGGCTCAGTTCAGCAGGAACAG156750.11901969682968734No Hit
TCCTGTACTGAGCTGCCCCA154820.11755425494846694No Hit
AGAGGTAGTAGGTTGCATAGTT153630.11665069233776629No Hit
CAACGGAATCCCAAAAGCAGCTG153090.11624067232954918No Hit
TCCTGTACTGAGCTGCCCCGG150840.11453225562864458No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT143700.10911087996444063No Hit
AGAGGTAGTAGGTTGCATAGT140640.10678743325121037No Hit
TATTGCACTTGTCCCGGCCTGTA140580.10674187547251958No Hit
GGCTGGTCCGATGGTAGTGGGTT139380.10583071989870378No Hit
TGGAGTGTGACAATGGTGTTTG136830.10389451430434524No Hit
TCAGTGCACTACAGAACTTTGG136620.10373506207892748No Hit
TGAGGGGCAGAGAGCGAGACTTC135990.10325670540267419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)